Staphylococcal enterotoxin A regulates bone marrow granulocyte trafficking during pulmonary inflammatory disease in mice

Takeshita, W.M.; Gushiken, V.O.; Ferreira-Duarte, A.P.; Pinheiro-Torres, A.S.; Roncalho-Buck, I.A.; Squebola-Cola, Dalize M.; Mello, Gláucia C.; Anhê, Gabriel Forato; Antunes, Edson; DeSouza, Ivani A.

doi:10.1016/j.taap.2015.06.013

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Myeloid proliferation or/and prevention of discharge from the bone marrow into the circulation are hypothesized to cause an increase in myeloid line cells. Data on the impact of hormone administration on the bone marrow is supported by several investigations [25]. Our conclusions were reinforced by 2015 research by Ayodeji on the age-dependency of blood parameters and biochemical markers during healing chronic toxicity.…”

Section: A -Anthraquinones B -Flavonoidssupporting

confidence: 59%

Effect of the complex extract from Rumex plants on quantitative parameters of blood cells and bone marrow in vivo

Shokan,

Yergozova,

T.N. Kobylina

et al. 2024

IJBCh

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Assessment of the composition of peripheral blood cells and red bone marrow in preclinical studies is a key component of program for analysis of biologically active compounds obtained from the plant materials. Current study concerns effect of the complex extract from Rumex plants (R. confertus, R. tianschanicus, R. thyrsiflorus) on parameters of blood cells and bone marrow of laboratory animals. During the study, data were obtained on the effect of the extract on hematopoiesis when administered at 100 mg/kg of animal weight. The results obtained show that application of the extract is accompanied by a statistically significant increase in the number of neutrophil precursors, such as myeloblasts and band neutrophils (p≤0.05). According to our results the extract we obtained from Rumex plants does not cause inhibition of early bone marrow precursors and is safe and non-toxic.

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Section: A -Anthraquinones B -Flavonoidssupporting

confidence: 59%

Effect of the complex extract from Rumex plants on quantitative parameters of blood cells and bone marrow in vivo

Shokan,

Yergozova,

T.N. Kobylina

et al. 2024

IJBCh

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“…Next, BM cells were incubated with SEA (1–30 ng/ml), IFN-γ (10 ng/ml) or sterile IMDM ( control group) at 37°C, 5% CO 2 for 30 min to 4 h. Adhesion and chemotaxis assays for neutrophils were performed as described below. For the adhesion assays, 50 μL of BM cells, treated or not with SEA, were added to 96-well plates pre-coated with recombinant mouse VCAM-1 (2.5 μg/ml) or ICAM-1 (2.5 μg/ml) (Takeshita et al, 2015 ) . BM neutrophils were then stimulated with IL-8 (300 ng/ml) for 30 min, and adhesion was calculated by measuring the myeloperoxidase (MPO) activity of adherent cells.…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular Ca 2+ levels were monitored in non-adherent BM cells (1 × 10 6 cells/ml) loaded with a fluorogenic calcium-binding dye (FluoForte), according to a previous study (Takeshita et al, 2015 ). BM cells were incubated with SEA (10 ng/ml), and suspended in a medium containing Fluoforte (3 μM) for 45 min at room temperature protected from light.…”

Section: Methodsmentioning

confidence: 99%

MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

Ferreira-Duarte

Pinheiro-Torres

Anhê

et al. 2017

Front. Cell. Infect. Microbiol.

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Staphylococcal enterotoxins are classified as superantigens that act by linking T-cell receptor with MHC class II molecules, which are expressed on classical antigen-presenting cells (APC). Evidence shows that MHC class II is also expressed in neutrophils and eosinophils. This study aimed to investigate the role of MHC class II and IFN-γ on chemotactic and adhesion properties of neutrophils and eosinophils after incubation with SEA. Bone marrow (BM) cells obtained from BALB/c mice were resuspended in culture medium, and incubated with SEA (3–30 ng/ml; 1–4 h), after which chemotaxis and adhesion were evaluated. Incubation with SEA significantly reduced the chemotactic and adhesive responses in BM neutrophils activated with IL-8 (200 ng/ml). Likewise, SEA significantly reduced the chemotactic and adhesive responses of BM eosinophils activated with eotaxin (300 ng/ml). The inhibitory effects of SEA on cell chemotaxis and adhesion were fully prevented by prior incubation with an anti-MHC class II blocking antibody (2 μg/ml). SEA also significantly reduced the intracellular Ca2+ levels in IL-8- and eotaxin-activated BM cells. No alterations of MAC-1, VLA4, and LFA-1α expressions were observed after SEA incubation. In addition, SEA elevated by 3.5-fold (P < 0.05) the INF-γ levels in BM cells. Incubation of BM leukocytes with IFN-γ (10 ng/ml, 2 h) reduced both neutrophil and eosinophil chemotaxis and adhesion, which were prevented by prior incubation with anti-MHC class II antibody (2 μg/ml). In conclusion, SEA inhibits neutrophil and eosinophil by MHC class II-dependent mechanism, which may be modulated by concomitant release of IFN-γ.

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“…Previous studies showed that administration of S. aureus enterotoxin in animal models resulted in acute pulmonary inflammation (17,58,62,63), and this response appeared to be mediated by T cells (27,34,54). In particular, inhalation of S. aureus enterotoxin first induced a systemic inflammatory response characterized by rapid T-cell activation, cytokine and chemokine release, and a T cell-orchestrated recruitment of innate immune cells into the circulation, lymphoid tissues, and lung (34,63,76,77). This early response occurring within several hours of S. aureus enterotoxin exposure was followed by development of considerable lung pathology at 48 h after inhalation, which was marked by a massive T-cell expansion in lymphoid tissues and lung (54,63).…”

mentioning

confidence: 99%

Therapeutic blockade of CD54 attenuates pulmonary barrier damage in T cell-induced acute lung injury

Svedova

Ménoret

Mittal

et al. 2017

American Journal of Physiology-Lung Cellular and Molecular Phys

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Acute respiratory distress syndrome (ARDS) is a serious, often fatal condition without available pharmacotherapy. Although the role of innate cells in ARDS has been studied extensively, emerging evidence suggests that T cells may be involved in disease etiology. enterotoxins are potent T-cell mitogens capable of triggering life-threatening shock. We demonstrate that 2 days after inhalation of enterotoxin A, mice developed T cell-mediated increases in vascular permeability, as well as expression of injury markers and caspases in the lung. Pulmonary endothelial cells underwent sequential phenotypic changes marked by rapid activation coinciding with inflammatory events secondary to T-cell priming, followed by reductions in endothelial cell number juxtaposing simultaneous T-cell expansion and cytotoxic differentiation. Although initial T-cell activation influenced the extent of lung injury, CD54 (ICAM-1) blocking antibody administered well after enterotoxin exposure substantially attenuated pulmonary barrier damage. Thus CD54-targeted therapy may be a promising candidate for further exploration into its potential utility in treating ARDS patients.

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Staphylococcal enterotoxin A regulates bone marrow granulocyte trafficking during pulmonary inflammatory disease in mice

Cited by 6 publications

References 42 publications

Effect of the complex extract from Rumex plants on quantitative parameters of blood cells and bone marrow in vivo

Effect of the complex extract from Rumex plants on quantitative parameters of blood cells and bone marrow in vivo

MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

Therapeutic blockade of CD54 attenuates pulmonary barrier damage in T cell-induced acute lung injury

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