Staphylococcal Clumping With Soluble Fibrin Monomer Complexes

Lipiński, Bogusław; Hawiger, Jacek; Jeljaszewicz, J.

doi:10.1084/jem.126.5.979

Cited by 57 publications

(21 citation statements)

References 15 publications

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“…This way, the NADH oxidase enzyme system on the bacterial cytoplasmic membrane, which they propose is the normal target of cationic proteins, is by-passed. The development of resistance outside coagulase or clumping factor-fibrinogen interactions in this work could be by a similar mechanism or it could be due to purely chemical interactions between organisms and the various by-products of metabolism which accumulate during incubation (Lipinski et al 1967).…”

Section: Discussionmentioning

confidence: 85%

“…Zak-plasma at 10 h was, however, not agglutinated by antifibrinogen antiserum, suggesting that its resistance, slight surface coating and clumping were not due to fibrin formation on the surface. It is not known whether the strain Zak used here produces CCFAS-like S. aureus strains, but it is known to produce a protamine-like substance which is capable of paracoagulation with soluble fibrin monomers (Lipinski, Hawiger & Jeljaszewicz, 1967;) and which could be responsible for the observed resistance. Also, the observed resistance of organisms after prolonged incubation in BSA (22 h) or broth (24 h), both of which are not known to interact with coagulase or clumping factor, could only result from different, time-dependent mechanisms since organisms taken out at earlier times were susceptible.…”

Section: Discussionmentioning

confidence: 99%

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Mechanisms of resistance of staphylococci grown in plasma to polymorph bactericidins

Kolawole

1984

J. Hyg.

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SUMMARYThe mechanisms whereby staphylococcal strains grown in plasma assume increased resistance to polymorph bactericidins were investigated. Observations reported here showed that cultural conditions could determine the path of conversion to resistance. Staphylococcal strains and mutants lacking either free coagulase or clumping factor or both all showed enhanced resistance after 10 h incubation in plasma proteins, thus giving no clear indication that these factors were involved in the interactions. In fact, prolonged incubation in bovine serum albumin (22 h) and ordinary broth medium (24 h) also resulted in increased resistance. A distinction between staphylococcal factors interacting specifically with plasma proteins and such non-specific conversions was obtained in two different ways. Stripping of a hypothetical surface protein by treatment with trypsin or 2 M potassium bromide rendered plasma-but not 24 h-broth organisms susceptible, indicating protein coating of plasma-grown organisms. Also free coagulase-positive strains and mutants incubated in plasma for 30 min were converted while those lacking both or possessing clumping factor alone were not. It therefore appears that one of the mechanisms of acquiring resistance involves a rapid interaction between staphylococcal-free coagulase and fibrinogen, resulting in the deposition of fibrin or fibrin derivatives on the bacterial surface.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Mechanisms of resistance of staphylococci grown in plasma to polymorph bactericidins

Kolawole

1984

J. Hyg.

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“…in the posttreatment specimens. The staphylococcal clumping test is insensitive to the lower molecular weight fragments D and E (Allington, 1967;Lipinski et al, 1967;Hawiger et al, 1970;Thomas et al, 1970;Leavelle et al, 1971;Marder et al, 1971). The high concentrations of D and E before treatment are thought to be due to lysis and subsequent excretion of deposits of intraglomerular fibrin.…”

Section: Discussionmentioning

confidence: 99%

Modification by Drugs of Urinary Fibrin Fibrinogen Degradation Products in Glomerulonephritis

Clarkson¹,

MacDonald²,

Cash³

et al. 1972

BMJ

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“…The strain used in this study was S. aureus Newman (35). S. aureus RN4220 (36) and Escherichia coli DH5␣ (37) were used for cloning purposes.…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of mreB Expression in Staphylococcus aureus via a Collection of New Integrative Plasmids

Yepes

Koch

Waldvogel

et al. 2014

Appl Environ Microbiol

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Protein localization has been traditionally explored in unicellular organisms, whose ease of genetic manipulation facilitates molecular characterization. The two rod-shaped bacterial models Escherichia coli and Bacillus subtilis have been prominently used for this purpose and have displaced other bacteria whose challenges for genetic manipulation have complicated any study of cell biology. Among these bacteria is the spherical pathogenic bacterium Staphylococcus aureus. In this report, we present a new molecular toolbox that facilitates gene deletion in staphylococci in a 1-step recombination process and additional vectors that facilitate the insertion of diverse reporter fusions into newly identified neutral loci of the S. aureus chromosome. Insertion of the reporters does not add any antibiotic resistance genes to the chromosomes of the resultant strains, thereby making them amenable for further genetic manipulations. We used this toolbox to reconstitute the expression of mreB in S. aureus, a gene that encodes an actin-like cytoskeletal protein which is absent in coccal cells and is presumably lost during the course of speciation. We observed that in S. aureus, MreB is organized in discrete structures in association with the membrane, leading to an unusual redistribution of the cell wall material. The production of MreB also caused cell enlargement, but it did not revert staphylococcal shape. We present interactions of MreB with key staphylococcal cell wall-related proteins. This work facilitates the use S. aureus as a model system in exploring diverse aspects of cellular microbiology. A significant number of studies of protein localization has been traditionally performed by using bacterial models, which are relatively simple and tractable haploid organisms (1-3). However, only a few bacterial species, such as the model organisms Escherichia coli and Bacillus subtilis, are generally considered for these studies, mostly because of the availability of a large number of molecular tools. In an attempt to have a broader and more general idea of the fundamental processes within the microbial world, there is growing interest in exploring other bacterial species that differ in shape, developmental properties, or infective potential from conventional bacterial models (4-6). Among these species is the spherical bacterium Staphylococcus aureus. It is an opportunistic pathogen that causes hard-to-treat acute and chronic infections in humans (7). It is indeed the study of its pathogenic attributes that makes this bacterium very attractive. Besides the interest in S. aureus as a major human pathogen, it is also an appealing model to explore questions related to cell shape, cell division, and cell proliferation, because it propagates very efficiently under laboratory conditions and is morphologically different from the conventional models E. coli and B. subtilis.In order to genetically manipulate S. aureus in the laboratory, several batteries of plasmids have been constructed over the past years (8-12), yet performance of p...

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Staphylococcal Clumping With Soluble Fibrin Monomer Complexes

Cited by 57 publications

References 15 publications

Mechanisms of resistance of staphylococci grown in plasma to polymorph bactericidins

Mechanisms of resistance of staphylococci grown in plasma to polymorph bactericidins

Modification by Drugs of Urinary Fibrin Fibrinogen Degradation Products in Glomerulonephritis

Reconstruction of mreB Expression in Staphylococcus aureus via a Collection of New Integrative Plasmids

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