Stanniocalcin: A molecular guard of neurons during cerebral ischemia

Zhang, Ke-zhou; Lindsberg, Perttu J.; Tatlısumak, Turgut; Kaste, Markku; Olsen, Henrik S.; Andersson, Leif C.

doi:10.1073/pnas.97.7.3637

Cited by 142 publications

(67 citation statements)

References 32 publications

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“…Intriguingly, Zhang et al (2000) found that STC1 expression in the human adult neuronal tumor cell line, Paju, conferred resistance to hypoxia, and consequently proposed that STC1 acts as a 'molecular guard', protecting cells, rather than promoting cell death as we have found in our study. It is possible that the conflicting observations relating to STC1's impact upon cell survival arise from cell-type-specific effects: terminally differentiated cells, such as adult neurons, need to survive for the life-time of the organism and hence rarely undergo apoptosis (Benn and Woolf, 2004).…”

Section: Regulation Of Oxidative Stress Response By Stc1supporting

confidence: 71%

“…To determine whether the upregulation of STC1 by other stimuli reduces cell survival, we induced hypoxia with cobalt chloride because hypoxia had previously been found to induce STC1 expression in cancer cells (Zhang et al, 2000;Yeung et al, 2005). As in the cancer cells, STC1 mRNA expression was induced in STC1 þ / þ MEFs by hypoxia (Supplementary Figure S3A), and we found that STC1À/À MEFs exhibited greater survival than STC1 þ / þ MEFs (Supplementary Figure S3B).…”

Section: Regulation Of Oxidative Stress Response By Stc1 a Nguyen Et Almentioning

confidence: 67%

“…For example, STC1 expression is upregulated in response to hypoxia (Zhang et al, 2000;Lal et al, 2001), and osmotic stress (Sheikh-Hamad et al, 2000). In an in vitro model of wound healing, STC1 expression was found to be rapidly upregulated following provision of serum to serum-starved human fibroblasts (Iyer et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Stanniocalcin-1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress

2009

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Mammalian Stanniocalcin-1 (STC1) is a glycoprotein that has been implicated in various biological processes including angiogenesis. Aberrant STC1 expression has been reported in breast, ovarian and prostate cancers, but the significance of this is not well understood. Here, we report that oxidative stress caused a 40-fold increase in STC1 levels in mouse embryo fibroblasts (MEFs). STC1À/À MEFs were resistant to growth inhibition and cell death induced by H 2 O 2 or by 20% O 2 (which is hyperoxic for most mammalian cells); this is the first phenotype reported for STC1-null cells. STC1À/À cells had higher levels of activated MEK and ERK1/2 than their wild-type (WT) counterparts, and these levels were all reduced by stable expression of exogenous STC1 in STC1À/À cells. Furthermore, pharmacological inhibition by PD98059 or UO126 of MEK and therefore of ERK1/2 activation restored sensitivity of STC1À/À cells to oxidative stress. We also found that H 2 O 2 -induced STC1 expression in WT cells was abolished by inhibition of ERK1/2 activation. Thus, the ERK1/2 signaling pathway upregulates STC1 expression, which in turn downregulates the level of activated MEK and consequently ERK1/2 in a novel negative feedback loop. Therefore, STC1 expression downregulates prosurvival ERK1/2 signaling and reduces survival under conditions of oxidative stress.

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Section: Regulation Of Oxidative Stress Response By Stc1supporting

confidence: 71%

Section: Regulation Of Oxidative Stress Response By Stc1 a Nguyen Et Almentioning

confidence: 67%

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Stanniocalcin-1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress

2009

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“…53 Overexpression of the stanniocalcin 1 (STC1) gene has previously been described in breast cancer where its expression level significantly correlated with the number of involved lymph nodes. 54 STC1 was also shown to be induced by hypoxia and hypertonicity, increasing resistance to ischemic situations 55 probably by mediating inflammation or angiogenesis. 56 The third gene confirmed by RT-PCR, LIMK2, has 2 LIM domains.…”

Section: Discussionmentioning

confidence: 99%

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Missiaglia

Blaveri

Terris

et al. 2004

Intl Journal of Cancer

139

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Pancreatic cancer is a highly aggressive type of malignancy and the prognosis for disease presenting typically at a late stage is extremely poor. A comprehensive understanding of its molecular genetics is required in order to develop new approaches to clinical management. To date, serial analysis of gene expression and more recently oligo/cDNA microarray technologies have been employed in order to identify genes involved in pancreatic neoplasia that can be developed as diagnostic markers and drug targets for this dismal disease. This study describes the expression profile obtained from 20 pancreatic cell lines using cDNA microarrays containing 9,932 human gene elements. Numerous genes were identified as being differentially expressed, some of which have been previously implicated in pancreatic adenocarcinoma (S100P, S100A4, prostate stem cell antigen, lipocalin 2, claudins 3 and 4, trefoil factors 1 and 2) as well as several novel genes. The differentially expressed genes identified are involved in a variety of cellular functions, including control of transcription, regulation of the cell cycle, proteolysis, cell adhesion and signaling. Validation of our array results was performed by exploring the SAGEmap database and by immunohistochemistry for a selection of 4 genes that have not previously been studied in pancreatic cancer: anterior gradient 2 homologue (Xenopus laevis), insulin-like growth factor binding protein 3 and 4 and Forkhead box J1. Immunostaining was performed using pancreas-specific tissue microarrays containing core biopsies from 305 clinical specimens. In addition, using statistical group comparison and hierarchical clustering, a selection of genes was identified that may be linked to the site of metastasis from which these cell lines were isolated.

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“…STC1 가지며 체내 인산의 재흡수를 촉진하고 칼슘의 과잉 흡수를 , 억제한다 (Madsen et al, 1998;Olsen et al, 1996; 현재까지 보고된 포유류 에 대한 보고에 의하 al., 1997). STC1 면 은 항과잉칼슘 작용을 할 뿐만 아니라 대뇌신경세포 , STC1 , , 조골세포 및 지방세포의 분화 후기에 특이적으로 높은 발현 수준을 보이며 난소의 과립막 세포에서 곁분비되어 마우스 , 난소의 성 스테로이드 분비를 조절한다 (Filvaroff et al, 2002;Luo et al, 2004;Zhang et al, 2000). 어류의 단백질은 연어과 어류에서 최초로 분리되었 STC1 으며 (Lafeber et al, 1988;Sundell et al, 1992;Wagner et al, 염기서열은 연어류 고대어종 1986;Yamashita et al, 1995), , 유럽넙치 (holostean), (Platichthys flesus 넙치 ), (Paralichthys olivaceus 터봇 ), (Scophthalmus maximus 등의 다양한 어종에서 ) 확인되었다 (Amemiya et al, 2002;Amemiya and Youson, 2004; 어류의 Hang and Balment, 2005;Shin et al, 2006;2008 Ishibashi and Masachi, 2002;Shin et al, 2006;2008).…”

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Effect of Recombinant Olive Flounder Stanniocalcin on Serum Calcium Levels

Shin¹,

Jung²,

Han³

et al. 2010

Korean Journal of Fisheries and Aquatic Sciences

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Stanniocalcin 1 (STC1) is a glycoprotein hormone that is important in the maintenance of calcium and phosphate homeostasis in both fish and mammals. STC1 and its paralog STC2 are expressed in multiple tissues in fishes, although the physiological roles of piscine STCs are still unclear compared with those of mammals. In this study, we cloned olive flounder STC1 (ofSTC1) and ofSTC2 cDNAs into pET28a vector and used E. coli Rosetta (DE3) as the host strain for protein expression. Expression experiments were carried out using isopropyl--D-thiogalactoside (IPTG) and nickel affinity chromatography. We could β identify the recombinant proteins as single 29.5 kDa (ofSTC1) and 33.2 kDa (ofSTC2) bands in the insoluble fraction on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These results indicate that ofSTC1 and ofSTC2 were expressed as insoluble proteins in E. coli. Furthermore, the injection of ofSTC1 protein into juvenile tilapia resulted in a decrease of the serum calcium level. These results suggest that the purified fish STC1 and STC2 proteins may be used to elucidate the physiological role of STCs in fishes.

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Stanniocalcin: A molecular guard of neurons during cerebral ischemia

Cited by 142 publications

References 32 publications

Stanniocalcin-1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress

Stanniocalcin-1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Effect of Recombinant Olive Flounder Stanniocalcin on Serum Calcium Levels

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