Standardized static and dynamic evaluation of myocardial tissue properties

Ramadan, Sherif; Paul, Narinder; Naguib, Hani E.

doi:10.1088/1748-605x/aa57a5

Cited by 44 publications

(31 citation statements)

References 39 publications

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“…Finally, since the experiments were performed in an in vitro condition, it is reasonable to assume that the cardiac tissue got stiffer during preparation. We thus used in our simulations the Young's modulus of the cardiac tissue at systole, which is within the range of measurements in an in vitro experimental setup found in Ramadan …”

Section: Calibration Of the Modelmentioning

confidence: 90%

A computational model of open‐irrigated radiofrequency catheter ablation accounting for mechanical properties of the cardiac tissue

Petras

Leoni

Guerra

et al. 2019

Numer Methods Biomed Eng

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Radiofrequency catheter ablation (RFCA) is an effective treatment for cardiac arrhythmias. Although generally safe, it is not completely exempt from the risk of complications. The great flexibility of computational models can be a major asset in optimizing interventional strategies, if they can produce sufficiently precise estimations of the generated lesion for a given ablation protocol. This requires an accurate description of the catheter tip and the cardiac tissue. In particular, the deformation of the tissue under the catheter pressure during the ablation is an important aspect that is overlooked in the existing literature, that resorts to a sharp insertion of the catheter into an undeformed geometry. As the lesion size depends on the power dissipated in the tissue, and the latter depends on the percentage of the electrode surface in contact with the tissue itself, the sharp insertion geometry has the tendency to overestimate the lesion obtained, especially when a larger force is applied to the catheter. In this paper we introduce a full 3D computational model that takes into account the tissue elasticity, and is able to capture the tissue deformation and realistic power dissipation in the tissue. Numerical results in FEniCS-HPC are provided to validate the model against experimental data, and to compare the lesions obtained with the new model and with the classical ones featuring a sharp electrode insertion in the tissue.

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Section: Calibration Of the Modelmentioning

confidence: 90%

A computational model of open‐irrigated radiofrequency catheter ablation accounting for mechanical properties of the cardiac tissue

Petras

Leoni

Guerra

et al. 2019

Numer Methods Biomed Eng

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“…Our data show that the resulting multiscale scaffolds have suitable mechanical properties as natural myocardium to support cell transplantation until mature cardiovascular tissue formation due to the secondary alginate hydrogel and compartmentalized structure dividing the cell culture region (Fig. 2 ) 26 . From the perspective of mimicking actual cardiovascular tissue, this system was designed to achieve the formation of myocardial tissues and peripheral blood vessels without randomly culturing three kinds of cells inside the scaffolds.…”

Section: Discussionmentioning

confidence: 77%

Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers

Kook

Hwang

Kim

et al. 2020

Sci Rep

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We report a technique to reconstruct cardiovascular tissue using multiscale scaffolds incorporating polycaprolactone fibers with double-layered hydrogels comprising fibrin hydrogel surrounded by secondary alginate hydrogel. The scaffolds compartmentalized cells into the core region of cardiac tissue and the peripheral region of blood vessels to construct cardiovascular tissue, which was accomplished by a triple culture system of adipose-derived mesenchymal stem cells (ADSCs) with C2C12 myoblasts on polycaprolactone (PCL) fibers along with human umbilical vein endothelial cells (HUVECs) in fibrin hydrogel. The secondary alginate hydrogel prevented encapsulated cells from migrating outside scaffold and maintained the scaffold structure without distortion after subcutaneous implantation. According to in vitro studies, resultant scaffolds promoted new blood vessel formation as well as cardiomyogenic phenotype expression of ADSCs. Cardiac muscle-specific genes were expressed from stem cells and peripheral blood vessels from HUVECs were also successfully developed in subcutaneously implanted cell-laden multiscale scaffolds. Furthermore, the encapsulated stem cells modulated the immune response of scaffolds by secreting anti-inflammatory cytokines for successful tissue construction. Our study reveals that multiscale scaffolds can be promising for the remodeling and transplantation of cardiovascular tissue.

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“…A standardized handling of the samples was further assured by the highly standardized 3D-printed testing equipment and clamping procedures, which do not require time-consuming manual closure. Moreover, the preconditioning assured to align the extracellular matrix perpendicular to the direction of load application, which is known to increase the reproducibility of the obtained mechanical parameters by alleviating post mortem-, storage-, freezing-, thawing- or fixation-related influences [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

What Is Considered a Variation of Biomechanical Parameters in Tensile Tests of Collagen-Rich Human Soft Tissues?—Critical Considerations Using the Human Cranial Dura Mater as a Representative Morpho-Mechanic Model

et al. 2020

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Background and Objectives: Profound knowledge on the load-dependent behavior of human soft tissues is required for the development of suitable replacements as well as for realistic computer simulations. Regarding the former, e.g., the anisotropy of a particular biological tissue has to be represented with site- and direction-dependent particular mechanical values. Contrary to this concept of consistent mechanical properties of a defined soft tissue, mechanical parameters of soft tissues scatter considerably when being determined in tensile tests. In spite of numerous measures taken to standardize the mechanical testing of soft tissues, several setup- and tissue-related factors remain to influence the mechanical parameters of human soft tissues to a yet unknown extent. It is to date unclear if measurement extremes should be considered a variation or whether these data have to be deemed incorrect measurement outliers. This given study aimed to determine mechanical parameters of the human cranial dura mater as a model for human soft tissues using a highly standardized protocol and based on this, critically evaluate the definition for the term mechanical “variation” of human soft tissue. Materials and Methods: A total of 124 human dura mater samples with an age range of 3 weeks to 94 years were uniformly retrieved, osmotically adapted and mechanically tested using customized 3D-printed equipment in a quasi-static tensile testing setup. Scanning electron microscopy of 14 samples was conducted to relate the mechanical parameters to morphological features of the dura mater. Results: The here obtained mechanical parameters were scattered (elastic modulus = 46.06 MPa, interquartile range = 33.78 MPa; ultimate tensile strength = 5.56 MPa, interquartile range = 4.09 MPa; strain at maximum force = 16.58%, interquartile range = 4.81%). Scanning electron microscopy revealed a multi-layered nature of the dura mater with varying fiber directions between its outer and inner surface. Conclusions: It is concluded that mechanical parameters of soft tissues such as human dura mater are highly variable even if a highly standardized testing setup is involved. The tissue structure and composition appeared to be the main contributor to the scatter of the mechanical parameters. In consequence, mechanical variation of soft tissues can be defined as the extremes of a biomechanical parameter due to an uncontrollable change in tissue structure and/or the respective testing setup.

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Standardized static and dynamic evaluation of myocardial tissue properties

Abstract: Static analysis of the mechanical properties of myocardial tissue confirms that; preconditioning is necessary for reproducibility, and DMA provides a platform for reproducible testing of soft biological tissues.

Cited by 44 publications

References 39 publications

A computational model of open‐irrigated radiofrequency catheter ablation accounting for mechanical properties of the cardiac tissue

A computational model of open‐irrigated radiofrequency catheter ablation accounting for mechanical properties of the cardiac tissue

Cardiovascular tissue regeneration system based on multiscale scaffolds comprising double-layered hydrogels and fibers

What Is Considered a Variation of Biomechanical Parameters in Tensile Tests of Collagen-Rich Human Soft Tissues?—Critical Considerations Using the Human Cranial Dura Mater as a Representative Morpho-Mechanic Model

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