Standardized Polyalthia longifolia leaf extract (PLME) inhibits cell proliferation and promotes apoptosis: The anti-cancer study with various microscopy methods

Vijayarathna, Soundararajan; Chen, Yeng; Kanwar, Jagat R.; Sasidharan, Sreenivasan

doi:10.1016/j.biopha.2017.04.112

Cited by 24 publications

(21 citation statements)

References 45 publications

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“…Therefore, membrane blebbing should be the phenomenon of late apoptosis, rather than the cause of increased surface roughness in early apoptosis. Vijayarathna et al reported that membrane blebbing was observed by SEM at about 12 h after apoptosis induction [Vijayarathna et al, 2017]. This finding is basically consistent with the results that the caspases were obviously activated at approximately 16 h after the HeLa cells were treated by CB [Kulms et al, 2002; Hwang et al, 2013].…”

Section: Discussionsupporting

confidence: 83%

“…The ultrastructural morphology observed (Fig. 6) was also different from that of the membrane blebbing observed by Vijayarathna et al [Vijayarathna et al, 2017]. In fact, fluorescence staining and its intensity analysis indicated that the F-actin cytoskeleton disrupted rapidly, and the actin fragments filled the cells after the BMSCs were treated by CB (Fig.…”

Section: Discussionmentioning

confidence: 66%

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Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Wang

Bao

et al. 2019

Preprint

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 66%

Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Wang

Bao

et al. 2019

Preprint

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“…The ultrastructural morphology observed (Fig. 7) was also different from that of the membrane blebbing observed by Vijayarathna et al [Vijayarathna et al, 2017]. In fact, fluorescence staining and its intensity analysis indicated that the F-actin cytoskeleton disrupted rapidly, and the F-actin fragments filled the cells after the BMSCs were treated by CB (Fig.…”

Section: Biology Open • Accepted Manuscriptmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Zhou

Bao

et al. 2020

Biology Open

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statementCellular mechanical damage is connected with the apoptosis of BMSCs, and the alterations in mechanics and nanomorphology may be a sensitive index to detect alterations in cell viability during apoptosis. AbstractStem cell apoptosis exists widely in embryonic development, tissue regeneration, repair, aging and pathophysiology of disease. The molecular mechanism of stem cell apoptosis has been extensively investigated. However, alterations in biomechanics and nanomorphology have rarely been studied.Therefore, an apoptosis model was established for bone marrow mesenchymal stem cells (BMSCs) and the reconstruction of the mechanical properties and nanomorphology of the cells were investigated in detail. Atomic force microscopy (AFM), scanning electron microscopy (SEM), laser scanning confocal microscopy (LSCM), flow cytometry and Cell Counting Kit-8 analysis were applied to assess the cellular elasticity modulus, geometry, nanomorphology, cell surface ultrastructure, biological viability and early apoptotic signal (phosphatidylserine, PS). The results indicated that the cellular elastic modulus and volume significantly decreased, whereas the cell surface roughness obviously increased during the first 3 h of cytochalasin B (CB) treatment.Moreover, these alterations preceded the exposure of biological apoptotic signal PS. These findings suggested that cellular mechanical damage is connected with the apoptosis of BMSCs, and the alterations in mechanics and nanomorphology may be a sensitive index to detect alterations in cell viability during apoptosis. The results contribute to a further understanding of the apoptosis from the perspective of cell mechanics. Biology Open • Accepted manuscript

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“…The drugs with apoptosis inducing property and inhibiting the tumor progression in cancer, are considered as novel anticancer chemotherapeutic drugs (Singh et al, 2016). There are several cytoplasmic vacuoles induced after treatment with BLEA in breast cancer (MCF-7) cells which indicating the induction of apoptosis like cell death (Mallick et al, 2015; Vijayarathna et al, 2017). Double staing with AO-EtBr, followed by fluorescence microscopy for detection mode of cell death and differentiation of untreated cells, early apoptotic cells, late apoptotic cells and necrotic cells are indicating BLEA induced apoptosis in MCF-7 cells.…”

Section: Discussionmentioning

confidence: 99%

Semecarpus anacardiumLinn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

Singh¹,

Ranjan²,

Tripathi³

et al. 2020

Preprint

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Background: Semecarpus anacardium Linn. is a commonly used Ayurvedic medicinal plant. Their different parts including nuts, have various medicinal properties to treat clinical ailments such as vitiligo, inflamation, microbial infection, geriatric problem, baldness and neuro-related problems.Aim of the study: The main objective of this study was an evaluation of the anticancer activity of the leaves of Semecarpus anacardium Linn and phytochemical screening. Materials and methods: The phytochemical screening was carried out through GC-MS analysis. Cytotoxicity was examined using MTT assay whereas mode of cell death evaluated by fluorescence microscopy. Finally, antitumor activity was determined in EAC cell-induced tumorbearing mice. Results: The ethyl acetate extract induced cytotoxicity in cancer cells in a dose dependent manner (IC 50 : 0.57 µg/ml in MCF-7 cells) in different cancer cell lines. However, normal cells were relatively insensitive to the extract. Fluorescence microscopy results, confirmed that the extract induced apoptosis in cancer cells effectively. The extract induce cell cycle arrest at the G1 phase and suppressed cancer cell migration. An oral administration of the extract suppressed the tumor growth in the mice model bearing Ehrlich ascites carcinoma cells. Whereas, the ethyl acetate extract was also found to prolong the survival of tumor-bearing mice. Conclusion: Overall, these observations suggest the anticancer activities of the ethyl acetate extract of S. anacardium leaves. The study opens a new window to examine the phytochemical constituents from the leaves as a source for the anticancer compounds.

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Standardized Polyalthia longifolia leaf extract (PLME) inhibits cell proliferation and promotes apoptosis: The anti-cancer study with various microscopy methods

Cited by 24 publications

References 45 publications

Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy

Semecarpus anacardiumLinn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

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