Standardized Peptidome Profiling of Human Serum for the Detection of Pancreatic Cancer

Abstract

Standardized serum peptidome profiling could be a useful tool to improve biochemical diagnosis of PC in combination with the classic tumor marker, CA 19-9.

Usefulness of MALDI-TOF/MS Identification of Low-MW Fragments in Sera for the Differential Diagnosis of Pancreatic Cancer

“…Of the 160 peaks, 16 (10%) features were significantly correlated with the presence of diseases of the pancreas, namely PaCa and ChrPa and only 2 were closely correlated with tumor presence. Interestingly, the finding of an increased frequency of the 2305 m/z feature in PaCa is in line with previous findings made by Zapico-Muñiz et al, 22 who demonstrated an increased peak area for this feature in PaCa, by analyzing samples with MALDI-TOF/MS in positive linear mode. Likewise, 2 (m/z 1865 and 1450) among our first 10 selected most significant features (see Table 1) were also found by the same authors to be significantly correlated with PaCa.…”

Detection of pancreatic cancer using serum protein profiling

“…Clinical proteomics has emerged as a promising strategy to develop novel tools for biomarker strategies. [37][38][39][40][41][42] In this study, high-resolution MS profiling of human serum was used, with the aim of detecting specific patterns present in patients with PC. Using this method, a set of seven peptides was found that differentiated PC from healthy volunteers with a sensitivity of 78% and a specificity of 89%.…”

Peptidomics to study age-related diseases: spotlight on cancer and neurodegeneration

“…In the authors' view, the additional application of this peptide strongly improves the diagnostic power of commonly used serum tumour marker panel comprising of CA 19-9 and CEA, which might have additional implication in differential diagnosis of pancreatic cancer and pancreatitis [52]. Similarly, Zapico-Muñiz et al [53] developed standardised protocol to analyse peptidome profiles of human serum for detection of pancreatic cancer. Serum peptides were extracted using immobilized copper ion chromatography followed by MALDI mass spectrometry analysis.…”

“…The authors reported that the diagnostic sensitivity was 89.9%, and the diagnostic specificity was 92.7%, using two serum features and CA 19-9 serum concentration. By using this approach, the authors were able to distinguish between healthy controls and patients with pancreatic cancer and chronic pancreatitis, with the use of three features of the peptidome (diagnostic sensitivity, 98.2%; diagnostic specificity, 97.1%) [53].…”