Standardized molecular typing of <i>Candida albicans</i> strains

Lischewski, A.; Harmsen, Dag; Hacker, Jörg; Morschhäuser, Joachim

doi:10.1111/j.1439-0507.1997.tb00252.x

Cited by 7 publications

(10 citation statements)

References 9 publications

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“…The same arguments can be made for correlating drug resistance and genotype. Data continue to mount that drugresistant strains and species are emerging (236,259,285,300,312), especially in the setting of continued drug therapy, as in cases of recurrent oral-esophageal thrush in AIDS patients (21,190,226,269,419). At least two species, C. krusei and C. glabrata, have increased in frequency in immunodeficient patients, and at least one reason for their recent success may be their natural or acquired resistance to fluconazole (30,115,212,238,284,429,430).…”

Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

“…At least two species, C. krusei and C. glabrata, have increased in frequency in immunodeficient patients, and at least one reason for their recent success may be their natural or acquired resistance to fluconazole (30,115,212,238,284,429,430). However, there have also been studies demonstrating both strain replacement (21,190) and strain evolution (226,269). It is clear from these latter studies that resistant substrains can evolve from susceptible strains and that a resistant strain or species can replace a susceptible strain.…”

Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

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The Ins and Outs of DNA Fingerprinting the Infectious Fungi

2000

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SUMMARY DNA fingerprinting methods have evolved as major tools in fungal epidemiology. However, no single method has emerged as the method of choice, and some methods perform better than others at different levels of resolution. In this review, requirements for an effective DNA fingerprinting method are proposed and procedures are described for testing the efficacy of a method. In light of the proposed requirements, the most common methods now being used to DNA fingerprint the infectious fungi are described and assessed. These methods include restriction fragment length polymorphisms (RFLP), RFLP with hybridization probes, randomly amplified polymorphic DNA and other PCR-based methods, electrophoretic karyotyping, and sequencing-based methods. Procedures for computing similarity coefficients, generating phylogenetic trees, and testing the stability of clusters are then described. To facilitate the analysis of DNA fingerprinting data, computer-assisted methods are described. Finally, the problems inherent in the collection of test and control isolates are considered, and DNA fingerprinting studies of strain maintenance during persistent or recurrent infections, microevolution in infecting strains, and the origin of nosocomial infections are assessed in light of the preceding discussion of the ins and outs of DNA fingerprinting. The intent of this review is to generate an awareness of the need to verify the efficacy of each DNA fingerprinting method for the level of genetic relatedness necessary to answer the epidemiological question posed, to use quantitative methods to analyze DNA fingerprint data, to use computer-assisted DNA fingerprint analysis systems to analyze data, and to file data in a form that can be used in the future for retrospective and comparative studies.

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Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

2000

View full text Add to dashboard Cite

show abstract

Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

“…Data continue to mount that drugresistant strains and species are emerging (236,259,285,300,312), especially in the setting of continued drug therapy, as in cases of recurrent oral-esophageal thrush in AIDS patients (21,190,226,269,419). At least two species, C. krusei and C. glabrata, have increased in frequency in immunodeficient patients, and at least one reason for their recent success may be their natural or acquired resistance to fluconazole (30,115,212,238,284,429,430).…”

Section: Other Epidemiological Questions Amenable To Dna Fingerprintingmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Soll

2000

Clinical Microbiology Reviews

282

317

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“…Hybridization of chromosomal DNA with the CARE ‐2 probe was carried out as described previously [6].…”

Section: Methodsmentioning

confidence: 99%

Identification of CARE‐2‐negative Candida albicans isolates as Candida dubliniensis

Morschhäuser

Ruhnke

Michel

et al. 1999

Mycoses

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Among 302 clinical yeast isolates originally identified as Candida albicans, we found 16 isolates that did not hybridize with the C. albicans-specific repetitive DNA element CARE-2. These 16 isolates produced abundant chlamydospores, did not grow at 43 degrees C, and exhibited a distinct randomly amplified polymorphic DNA profile. Sequence analysis of part of the 28S rDNA demonstrated that the CARE-2-negative isolates are not an atypical subgroup of C. albicans but belong to the recently described new species C. dubliniensis.

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Standardized molecular typing of Candida albicans strains

Cited by 7 publications

References 9 publications

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Identification of CARE‐2‐negative Candida albicans isolates as Candida dubliniensis

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