Standardized full-field electroretinography in rabbits

Gjorloff, K.W.; Andréasson, Sten; Ehinger, Berndt

doi:10.1007/s10633-004-3924-5

Cited by 47 publications

(40 citation statements)

References 18 publications

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“…A standardized full-field electroretinography (ERG) was recorded 7 days before surgery and 28 days postoperatively on the right eye using a Nicolet Viking analysis system (Nicolet Biomedical Instruments, Madison Wisconsin) as previously described (Gjörloff et al 2004). …”

Section: Full-field Ergmentioning

confidence: 99%

Experimental vitreous tamponade using polyalkylimide hydrogel

Crafoord

Andréasson

Ghosh

2011

Graefes Arch Clin Exp Ophthalmol

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Purpose: To evaluate polyalkyl-imide as a possible vitreous tamponading agent. Methods:A 20 gauge pars plana vitrectomy and posterior vitreous detachment were performed in the right eye of six pigmented rabbits. Approximately 1 ml of viscoelastic gel, polyalkyl-imide (Bio-Alcamid ® ) was thereafter injected into the vitreous space. Full-field ERG and intraocular pressure (IOP, Tonopen) was measured pre-and postoperatively at regular intervals up to 28 days. At day 6 or 28, the rabbits were sacrificed and the eyes were examined macroscopically, photographed, and prepared for histological examination with routine microscopy. Results:The viscoelastic hydrogel was successfully injected and remained translucent with preserved gel properties throughout the postoperative period. The postoperative IOP was unchanged compared to preoperative values. Five of six eyes displayed retinal edema or pigmentary changes centrally while the periphery appeared intact. ERG recordings showed a radical decrease in rod and cone derived B-wave amplitudes. Histological examination confirmed varying degrees of edema combined with neuronal cell death within the retinal layers in the central part of the fundus while the peripheral part appeared intact. Conclusion:Polyalkyl-imide displays favourable physical properties when used as a vitreous tamponade. However, the hydrogel causes functional and morphological retinal damage when in direct contact with the inner retina. Possible pathological mechanisms include osmotic imbalance and direct toxic effects, and modification of biochemical properties is warranted before clinical use will be possible.

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Section: Full-field Ergmentioning

confidence: 99%

Experimental vitreous tamponade using polyalkylimide hydrogel

Crafoord

Andréasson

Ghosh

2011

Graefes Arch Clin Exp Ophthalmol

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“…Standardized full-field electroretinography (ERG) (slightly modified for rabbit ERG 53 ) was assessed on three occasions prior to treatment and on three occasions during treatment (2 months, analysis system (Nicolet Biomedical Instruments, Madison Wisconsin) as described previously. [52][53] , The examinations were conducted according to the International Society for Clinical Electrophysiology of Vision (ISCEV) standards.…”

Section: Electrophysiologymentioning

confidence: 99%

“…[52][53] , The examinations were conducted according to the International Society for Clinical Electrophysiology of Vision (ISCEV) standards. 54 The right eye was tested after maximal pupil dilation with topical 1% cyclopentolate hydrochloride, and after at least 30 minutes of dark adaptation.…”

Section: Electrophysiologymentioning

confidence: 99%

“…Responses were obtained with a wide band filter (-3dB at 1Hz and 500 Hz), stimulating with single full field flashes ( until two successive identical curves were obtained. 53 Oscillatory potentials were recorded from the dark adapted eye, with white light (integrated luminance 0.81cd-s/m 2 ) and by applying an overall band pass filter from 100 Hz (low frequency filter -LFF) to 300 Hz (high frequency filter -HFF). The referred luminance of the different light stimuli has been measured from a photometer on the light reflected from the Ganzfeld sphere.…”

Section: Electrophysiologymentioning

confidence: 99%

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Retinal function and histopathology in rabbits treated with Topiramate

et al. 2006

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Methods: Six rabbits were treated with a daily oral dose of topiramate orally during a period of eight months. Six rabbits receiving water served as controls. Blood samples were analyzed for determination of topiramate serum levels in order to ensure successful drug exposition.Standardized full-field electroretinograms (ERGs) were performed before treatment and then at 2, 3 and 8 months during the treatment period. After terminating treatment the rabbits were sacrificed and the morphology of the sectioned retina was studied. The retinal dysfunction and the morphological changes indicate that topiramat may damage the retina, similarly to vigabatrin (another anti-epileptic drug).

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“…We also wanted to explore cellular mechanisms behind any change in PKC alpha expression, and if possible relate such change to GFAP expression. To this end, we have used standardized protocols for full-field ERG and mfERG before and after treatment, combined with PKC alpha and GFAP immunohistochemistry as well as PKC alpha real-time PCR [10,11].…”

Section: Introductionmentioning

confidence: 99%