Standardized fraction of Xylocarpus moluccensis fruits improve vascular relaxation and plaque stability in dyslipidemic models of atherosclerosis

Kanshana, Jitendra S.; Rebello, Sanjay C.; Pathak, Priya; Kanuri, Babunageswararao; Aggarwal, Hobby; Srivastava, Vasundhara; Khanna, Vivek; Singh, Vishal; Jagavelu, Kumaravelu; Barthwal, Manoj Kumar; Dikshit, Madhu

doi:10.1016/j.jep.2017.11.004

Cited by 12 publications

(9 citation statements)

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“…Now this ethyl acetate fraction was coded as CDR‐267‐F018 or F018. This fraction majorly comprises xyloccensin A, xyloccensin X & Y, and xyloccensin E. The detailed characterization of the extract has been reported earlier by the laboratory (patent no: US 7,959,954 B2, Date: 14/6/11) …”

Section: Methodssupporting

confidence: 80%

“…This fraction majorly comprises xyloccensin A, xyloccensin X & Y, and xyloccensin E. The detailed characterization of the extract has been reported earlier by the laboratory (patent no: US 7,959,954 B2, Date: 14/6/11). [11,12] Induction of CIA and drug treatment…”

Section: Animals and Cell Culturementioning

confidence: 99%

“…Previous studies from the laboratory have demonstrated anti-atherosclerotic effect of X. moluccensis by modulating dyslipidaemia and inflammation [11] and have identified two limonoids: xyloccensin X and xyloccensin Y in the fruit of X. moluccensis. [12] Total content of these two identified molecules was 0.8-1% as indicated earlier also (patent no: US 7,959,954 B2, Date: 14/6/11).…”

Section: Introductionmentioning

confidence: 98%

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Standardized Xylocarpus moluccensis fruit fraction mitigates collagen-induced arthritis in mice by regulating immune response

Gupta

Kumar

Pal

et al. 2020

Journal of Pharmacy and Pharmacology

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Objective This study was undertaken to evaluate the effect of Xylocarpus moluccensis fruit fraction (F018) on the pathogenesis of collagen‐induced arthritis in mice. Methods Arthritis was induced by intradermal injection of collagen (2 mg/ml) with complete Freund’s adjuvant in DBA/1J mice. F018 was administered orally at 1, 3 and 10 mg/kg for 20 days. Disease progression and mechanism were assessed by micro‐CT analysis, RT‐PCR, flow cytometry assay, myeloperoxidase (MPO) and MTT assay. Results F018 at 3 and 10 mg/kg significantly reduced paw thickness, clinical score, mononuclear cell infiltration and collagen layer depletion in the knee section of collagen‐induced arthritis (CIA) mice when compared with collagen‐induced arthritis mice alone. Furthermore, F018 treatment in collagen‐induced arthritis mice significantly recovered bone volume and trabecular number and decreased the trabecular space by modulating RANKL and OPG mRNA expression in the synovial tissue. F018 treatment in collagen‐induced arthritis mice significantly attenuated spleen index, lymphocyte proliferation and paw myeloperoxidase (MPO) activity, pro‐inflammatory cytokine TNFα, IL1β, and IL6 mRNA expression and enhanced IL10 mRNA expression in paw tissue. Furthermore, F018 treatment in collagen‐induced arthritis mice significantly reduced splenic dendritic cell maturation and Th17 cells. In culture, F018 significantly decreased collagen‐induced arthritis‐FLS proliferation and promoted apoptosis. Conclusion F018 may serve as a potential curative agent for arthritis.

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Section: Methodssupporting

confidence: 80%

Section: Animals and Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Standardized Xylocarpus moluccensis fruit fraction mitigates collagen-induced arthritis in mice by regulating immune response

Gupta

Kumar

Pal

et al. 2020

Journal of Pharmacy and Pharmacology

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“…Liver tissue (50 mg) was processed as described previously for the estimation of hepatic total cholesterol [ 47 ], triglycerides [ 48 ], and free fatty acids [ 49 ] using Randox (Crumlin, Co. Antrim, UK) kit.…”

Section: Methodsmentioning

confidence: 99%

“…Formalin fixed, tissue freezing medium (Leica Biosystems, Ernst-Leitz-Strasse, Wetzlar, Germany) embedded frozen liver tissues were sectioned in 10 μm thin slices, stained with Oil Red O and counterstained with hematoxylin to visualize the lipid accumulation using Leica QWin version 3.5.1 software [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Systemic Insulin Resistance and Metabolic Perturbations in Chow Fed Inducible Nitric Oxide Synthase Knockout Male Mice: Partial Reversal by Nitrite Supplementation

et al. 2020

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iNOS, an important mediator of inflammation, has emerged as an important metabolic regulator. There are conflicting observations on the incidence of insulin resistance (IR) due to hyperglycemia/dyslipidemia in iNOS−/− mice. There are reports that high fat diet (HFD) fed mice exhibited no change, protection, or enhanced susceptibility to IR. Similar observations were also reported for low fat diet (LFD) fed KO mice. In the present study chow fed iNOS−/− mice were examined for the incidence of IR, and metabolic perturbations, and also for the effect of sodium nitrite supplementation (50 mg/L). In IR-iNOS−/− mice, we observed significantly higher body weight, BMI, adiposity, blood glucose, HOMA-IR, serum/tissue lipids, glucose intolerance, enhanced gluconeogenesis, and disrupted insulin signaling. Expression of genes involved in hepatic and adipose tissue lipid uptake, synthesis, oxidation, and gluconeogenesis was upregulated with concomitant downregulation of genes for hepatic lipid excretion. Nitrite supplementation restored NO levels, significantly improved systemic IR, glucose tolerance, and also reduced lipid accumulation by rescuing hepatic insulin sensitivity, glucose, and lipid homeostasis. Obesity, gluconeogenesis, and adipose tissue insulin signaling were only partially reversed in nitrite supplemented iNOS−/− mice. Our results thus demonstrate that nitrite supplementation to iNOS−/− mice improves insulin sensitivity and metabolic homeostasis, thus further highlighting the metabolic role of iNOS.

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Indian Academia Tryst with Cardio-metabolic Drug Discovery and Development

Barthwal

2021

Drug Discovery and Drug Development

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Standardized fraction of Xylocarpus moluccensis fruits improve vascular relaxation and plaque stability in dyslipidemic models of atherosclerosis

Cited by 12 publications

References 50 publications

Standardized Xylocarpus moluccensis fruit fraction mitigates collagen-induced arthritis in mice by regulating immune response

Standardized Xylocarpus moluccensis fruit fraction mitigates collagen-induced arthritis in mice by regulating immune response

Systemic Insulin Resistance and Metabolic Perturbations in Chow Fed Inducible Nitric Oxide Synthase Knockout Male Mice: Partial Reversal by Nitrite Supplementation

Indian Academia Tryst with Cardio-metabolic Drug Discovery and Development

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