Standardized Ethanol Extract of Cassia mimosoides var. nomame Makino Ameliorates Obesity via Regulation of Adipogenesis and Lipogenesis in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice
Abstract:Obesity is a major cause of conditions such as type 2 diabetes and non-alcoholic fatty liver disease, posing a threat to public health worldwide. Here, we analyzed the anti-obesity effects of a standardized ethanol extract of Cassia mimosoides var. nomame Makino (EECM) in vitro and in vivo. Treatment of 3T3-L1 adipocytes with EECM suppressed adipogenesis and lipogenesis via the AMP-activated protein kinase pathway by downregulating the expression levels of CCAAT/enhancer-binding protein-alpha, peroxisome proli… Show more
“…Although previous studies have demonstrated the significant roles of gut microbiota in regulating animal lipogenesis and fat deposition ( Zhao et al, 2020 ; Chen et al, 2021 ; Heo et al, 2023 ), there is a paucity of studies examining the correlations between gut microbiota and chicken lipogenesis. The HFD has been reported to contribute to the development of obesity by modulating gut microbiota composition.…”
“…Although previous studies have demonstrated the significant roles of gut microbiota in regulating animal lipogenesis and fat deposition ( Zhao et al, 2020 ; Chen et al, 2021 ; Heo et al, 2023 ), there is a paucity of studies examining the correlations between gut microbiota and chicken lipogenesis. The HFD has been reported to contribute to the development of obesity by modulating gut microbiota composition.…”
“…Proteins were extracted from differentiated 3T3-L1 cells, subcutaneous fat tissue, liver tissue, and brown adipose tissue as previously described. 37 The extracted proteins were quantified using the Bradford assay. The whole proteins (30 μg) were separated by SDS-PAGE using 8-15% acrylamide gel and then transferred to a PVDF membrane.…”
Obesity requires treatment to mitigate the potential development of further metabolic disorders, including diabetes, hyperlipidemia, tumor growth, and nonalcoholic fatty liver disease. We investigated the anti-obesity effect of a 30%...
“…The study authored by S. Heo et al delves into the potential of Cassia mimosoides var. nomame Makino extract (EECM) as a means to combat obesity [ 1 ]. EECM demonstrates its efficacy by inhibiting adipogenesis and lipogenesis, primarily through the AMP-activated protein kinase pathway.…”
Section: Obesity and Metabolic Disordersmentioning
Chronic diseases, encompassing conditions such as heart disease, cancer, and diabetes, represent a significant global health challenge and are the leading causes of mortality worldwide [...]
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