Standardization of Long Term DNA Storage and DNA Banking Methods for Conservation of Saudi Date Palm (Phoenix dactylifera L.) Genetic Diversity

Alturki, Saleh M.

doi:10.3923/ajps.2017.94.100

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…The DNA was resuspended in water containing 100 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −20°C. The quality and quantity of the isolated DNA were assessed by agarose gel electrophoresis and spectrophotometry [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand

et al. 2021

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Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2–3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV—which encodes the capsid protein—showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.

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Section: Methodsmentioning

confidence: 99%

Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand

et al. 2021

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“…The DNA was resuspended in water containing 100 μg/ml RNase (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −20C. The quality and quantity of the isolated DNA were assessed by agarose gel electrophoresis and spectrophotometry [21].…”

Section: Dna Extraction and Slcmv Detectionmentioning

confidence: 99%

Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand

Siriwan

Saokham

Hemniam

et al. 2021

Preprint

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Cassava plantations in an area of 458 ha spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease severity was generally scored as 2–3. The highest average disease severity was in Sakaeo province (3.7), followed by Buriram (3.6), Prachinburi (2.88), Surin (2.5), and Sisaket (2.4) provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Interestingly, cassava cultivars CMR-89 and Rayong 11 were susceptible to CMD. In approximately 95% of cases, the infection was transmitted by whitefly ( Bemisia tabaci ), which had a high population density in Prachinburi but was sparse in Surin, with the largest populations observed in May and June. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 ( mtCO1 ) gene of whitefly ( Bemisia tabaci ) in Thailand revealed a similarity to the Asia II 1 whitefly gene. Furthermore, the AV1 gene—which encodes the capsid protein—showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.

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Standardization of Long Term DNA Storage and DNA Banking Methods for Conservation of Saudi Date Palm (Phoenix dactylifera L.) Genetic Diversity

Cited by 2 publications

References 6 publications

Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand

Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand

Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand

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