Standardization of liquid formulation of Pseudomonas fluorescens Pf1 for its efficacy against Fusarium wilt of tomato

Manikandan, R.; Saravanakumar, D.; Rajendran, L.; Raguchander, T.; Samiyappan, R.

doi:10.1016/j.biocontrol.2010.04.004

Cited by 123 publications

(53 citation statements)

References 24 publications

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“…This last finding matches the work of Singleton et al (2002), who developed a liquid formula for Rhizobium by incorporating additives to the media for improved growth performances. Glycerol as an amendment is used because it holds a considerable amount of water and protects cells from desiccation by slowing the drying rate (Manikandan et al 2010). Yet, most additives are polymeric in nature, with high molecular weight.…”

Section: Liquid Inoculantsmentioning

confidence: 99%

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

Bashan¹,

de‐Bashan²,

et al. 2013

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Background Inoculation of plants to enhance yield of crops and performance of other plants is a century old, proven technology for rhizobia and a newer venue for plant growth-promoting bacteria and other plant symbionts. The two main aspects dominating the success of inoculation are the effectiveness of the bacterial isolate and the proper application technology. Scope An assessment of practical aspects of bacterial inoculants for contemporary agriculture and environmental restoration is critically evaluated from the point of view of their current technological status, current applications, and future use. This is done because there are windows of opportunity for new developments in applied research using renewable, non-contaminated natural resources and new venues for research. Special emphasis is given to formulations and polymeric carriers. This review concentrates on practical aspect of inoculation technology dating from 1998 to 2013. Earlier publications are mentioned only for clarification of a specific point. Conclusions This review discusses characteristics of a carrier for inoculants, formulations of inoculants including liquid, organic, inorganic, polymeric, and encapsulated formulations. Technical aspects include inoculation techniques (soil and seed application), mass culture production, bulk sterilization, seed coating, shelf-life, and effect of moisture. Future research venues needed are noted.

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Section: Liquid Inoculantsmentioning

confidence: 99%

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

Bashan¹,

de‐Bashan²,

et al. 2013

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“…The use of liquid media is another commonly employed method of producing biocontrol agents for commercial use. Liquid formulations have been extensively evaluated in the use of bacterial biocontrol agents such as Pseudomonas fluorescens (74) and Bacillus spp. (75) but not so extensively in the use of yeast-based biocontrol products.…”

Section: Exposure To Stress During Productionmentioning

confidence: 99%

Responses of Yeast Biocontrol Agents to Environmental Stress

Yuan

Wisniewski

Droby

et al. 2015

Appl Environ Microbiol

118

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c Biological control of postharvest diseases, utilizing wild species and strains of antagonistic yeast species, is a research topic that has received considerable attention in the literature over the past 30 years. In principle, it represents a promising alternative to chemical fungicides for the management of postharvest decay of fruits, vegetables, and grains. A yeast-based biocontrol system is composed of a tritrophic interaction between a host (commodity), a pathogen, and a yeast species, all of which are affected by environmental factors such as temperature, pH, and UV light as well as osmotic and oxidative stresses. Additionally, during the production process, biocontrol agents encounter various severe abiotic stresses that also impact their viability. Therefore, understanding the ecological fitness of the potential yeast biocontrol agents and developing strategies to enhance their stress tolerance are essential to their efficacy and commercial application. The current review provides an overview of the responses of antagonistic yeast species to various environmental stresses, the methods that can be used to improve stress tolerance and efficacy, and the related mechanisms associated with improved stress tolerance.

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“…The addition of polymers had a strong effect in the rate of cell death in all temperatures, and according to the Kumaresan and Reetha (2011) and Manikandan et al (2010), where they found that the polymers like glycerol, trehalose, and gum arabica doubled the shelf life of liquid inoculants of A. brasilense, and Pseudomonas fluorescens. Leo Daniel et al (2013) also found that the addition of polymers as additives and adjuvants maintain cell concentration in liquid inoculants of A. brasilsense, Bacillus megaterium and Azotobacter chroococcum, reducing just two or three log units in 480 days at room temperature.…”

Section: Cell Viability Prediction Using the Arrhenius Thermodynamic mentioning

confidence: 98%

Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model

and¹,

Rebeca²

2015

Afr. J. Biotechnol.

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Plant growth promoting bacteria (PGPB) enhances the growth of their hosts and can protect them from biotic and abiotic stresses. Bacterial inoculants contain one or more of these beneficial strains in a carrier material, which must be able to maintain the viability of the cells during the time of storage, and also guarantee the biological activity of the strains once applied in the soil. These inoculants can be solid, liquid, gel or oil-based, depending on the characteristics of the strains and the shelf life expected by the producers. In this study, we used a method of accelerated degradation to select a polymer and a concentration to maintain cell stability of a liquid inoculant based on the strain C16 Azospirillum brasilense. A screening at 45°C was made to compare the protectant effect of five polymers on the viability of the strain (p/v): carrageenan (1.5%), sodium alginate (1%), trehalose (10 mM), polyvinylpyrrolidone (2%), glycerol (10 mM) and phosphate saline buffer as control. Carrageenan and sodium alginate showed significant differences in cell viability over the use of other polymers (P < 0.05). We evaluated cell viability with these two polymers at three concentrations and three different temperatures (4, 28 and 45°C) for 60 days and determined the bacterial degradation rates. Based on the Arrhenius thermodynamic model, we calculated the time required to reduce cell concentration in three log units, and observed that the protectant activity of each polymer and each concentration depends on the temperature of storage. Cell viability was best preserved in all treatments at 4°C. In general, alginate prolonged cell viability at 28°C, and carrageenan at 45°C. Alginate at 1% and carrageenan at 0.75% showed a stable behavior (superior to the control) in the three evaluated temperatures, so we conclude that they can be used for a formulation of a liquid inoculant based on the strain C16 of A. brasilense.

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Standardization of liquid formulation of Pseudomonas fluorescens Pf1 for its efficacy against Fusarium wilt of tomato

Cited by 123 publications

References 24 publications

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

Responses of Yeast Biocontrol Agents to Environmental Stress

Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model

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