Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Wilkinson, J. H.; Baron, D N; Moss, D. W.; Walker, P. G.

doi:10.1136/jcp.25.11.940

Cited by 118 publications

(42 citation statements)

References 15 publications

(9 reference statements)

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“…2.6.1.2) [10], alkaline phosphatase (ALP E.C. 3.1.3.1) [11], and Cu-Zn superoxide dismutase (SOD E.C.…”

Section: Methodsmentioning

confidence: 99%

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Toxic effects of alcohol intake on prostate of rats

et al. 1997

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“…2.6.1.2) [10], alkaline phosphatase (ALP E.C. 3.1.3.1) [11], and Cu-Zn superoxide dismutase (SOD E.C.…”

Section: Methodsmentioning

confidence: 99%

“…The homogenate was centrifuged at 1,200g for 15 min. The supernatant was used for total protein [13], alanine transaminase [10], acid phosphatase (ACP-E.C.3.1.3.2.) [14], and lipoperoxide [15] determinations.…”

Section: Methodsmentioning

confidence: 99%

Toxic effects of alcohol intake on prostate of rats

et al. 1997

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“…Liver enzymes such as aspartate aminotransferase (AST) alanine aminotransferase (ALT) and alkaline phosphatase (ALP), and urea and creatinine levels of the patients were estimated as per standard techniques. The ALT and AST enzymes were estimated as per the method described by Wilkinson et al (1972) whereas the ALP was estimated as described earlier by Burtis and Asood (1999). The kidney function markers such as creatinine and urea were measured according to the procedure detailed by Thomas (1998) and Kalpan (1965), respectively.…”

Section: Methodsmentioning

confidence: 99%

Clinico-pathological studies of Plasmodium falciparum and Plasmodium vivax — malaria in India and Saudi Arabia

Khan

Zakai

Umm-e-Asma

2014

Acta Parasitologica

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Malaria is one of the most devastating diseases of tropical countries with clinical manifestations such as anaemia, splenomegaly, thrombocytopenia, hepatomegaly and acute renal failures. In this study, cases of thrombocytopenia and haemoglobinemia were more prominent in subjects infected with Plasmodium falciparum (Welch, 1897) than those with Plasmodium vivax (Grassi et Feletti, 1890). However, anaemia, jaundice, convulsions and acute renal failure were significantly high (3-4 times) in subjects infected with P. falciparum than those infected with P. vivax. The incidence of splenomegaly and neurological sequelae were 2 and 6 times higher in P. falciparum infections compared to the infections of P. vivax. Both in P. vivax and P. falciparum malaria, the cases of splenomegaly, jaundice and neurological sequelae were almost double in children (<10 years) compared to older patients. The liver enzymes were generally in normal range in cases of low and mild infections. However, the AST, ALT, ALP activities and serum bilirubin, creatinine, and the urea content were increased in P. falciparum and P. vivax malaria patients having high parasitaemia, confirming liver dysfunction and renal failures in few cases of severe malaria both in India and Saudi Arabia.

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“…The sera separated by centrifugation at 3000 rpm for 15 min were assayed for estimation of liver marker enzymes including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and total bilirubin in auto-analyser (Humalyzer-3500) by using reagent kits for ALT ( [15] were used to estimate ALT and AST in serum by measuring the decrease in absorbance of NADH at 340 nm, which is oxidized to NAD in the reaction involving conversion of L-alanine and L-aspartate to pyruvate and oxaloacetate. The ALP was estimated by measuring the concentration of p-nitro phenol formed from the hydrolysis of p-nitro phenyl phosphate by enzyme in the presence of oxidizing agent (Mg+²) and measured by absorbance at 405 nm proportional to ALP activity [16] .…”

Section: Biochemical Parametersmentioning

confidence: 99%

Comparative Hepatoprotective Activity of Fumaria farviflora Lam. Leaf Extract and Silymarin on Isoniazid and Rifampicin-induced Hepatotoxic Rats

Khan¹

2017

ijps

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Khan and Iqbal: Hepatoprotective Activity of Fumaria farviflora Lam. Leaves ExtractIsoniazid and rifampicin are first line drugs used for the treatment of tuberculosis but their use is associated with potentially serious toxic manifestation in the liver leading to necrosis, cirrhosis and hepatitis causing poor patient compliance. The present study was conducted to evaluate hepatoprotective activity of Fumaria parviflora leaf extract in isoniazid and rifampicin-induced hepatotoxic rats. Acute toxicity was conducted with single oral doses of F. parviflora leaf extracts at 300, 2000 and 5000 mg/kg and rats were observed for changes in body weight, haematological parameters, behavioural changes and signs of toxicity. Hepatoprotective activity of F. parviflora leaf extract (100, 200 and 300 mg/kg, p.o.) was evaluated on isoniazid and rifampicin (50 mg/kg, i.p.) induced hepatotoxic rats using silymarin as a reference drug (100 mg/kg, p.o.). F. parviflora leaf extract was found to be safe up to 5 g/kg. From biochemical and histopathological parameters it was found that pretreatment of rats with F. parviflora leaf extract an hour prior to start isoniazid and rifampicin resulted in a significant decline in the serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total bilirubin as well as normal histology of liver biopsy specimens. It was concluded that F. parviflora leaf extract has significant hepatoprotective activity at the dose of 200 mg/kg comparable with that of silymarin. The plant extract appeared to have the potential to be used as a dietary supplement to antiTB therapy to protect against the hepatotoxicity of isoniazid and rifampicin.

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Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Cited by 118 publications

References 15 publications

Toxic effects of alcohol intake on prostate of rats

Toxic effects of alcohol intake on prostate of rats

Clinico-pathological studies of Plasmodium falciparum and Plasmodium vivax — malaria in India and Saudi Arabia

Comparative Hepatoprotective Activity of Fumaria farviflora Lam. Leaf Extract and Silymarin on Isoniazid and Rifampicin-induced Hepatotoxic Rats

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