Standardization of Assays for Human Chorionic Gonadotropin

Stenman, Ulf‐Håkan

doi:10.1373/clinchem.2003.031013

Cited by 22 publications

(11 citation statements)

References 21 publications

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“…Standardisation and the establishment of a reference system [147] for the BTMs is the route to achieve this. IFCC has an extensive experience with this process [148][149][150][151][152].…”

Section: Standardisationmentioning

confidence: 99%

Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards

et al. 2010

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“…Standardisation and the establishment of a reference system [147] for the BTMs is the route to achieve this. IFCC has an extensive experience with this process [148][149][150][151][152].…”

Section: Standardisationmentioning

confidence: 99%

Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards

et al. 2010

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“…Other structural hCG isoforms, such as nicked or hyperglycosylated hCG, secreted by some carcinoma cells, have also been shown to interfere in these automated hCG assays (10,16,17). The higher hCG concentrations obtained with the AxSYM assay than those found for the Bayer automated assays (ADVIA-Centaur, ACS:180) may be attributed to differences in standardization, as previously reported (11). Moreover, our findings demonstrate that care should be taken when interpreting hCG measurements before transplantation to exclude pregnancy.…”

mentioning

confidence: 61%

“…However, recently demonstrated between-method variation in hCG results has been attributed to differences in assay specificity and to a lack of standardization (10,11). However, recently demonstrated between-method variation in hCG results has been attributed to differences in assay specificity and to a lack of standardization (10,11).…”

mentioning

confidence: 99%

Importance of the detection method for intact dimeric human chorionic gonadotropin without interference with the free human chorionic gonadotropin β subunit for pregnancy exclusion before liver transplantation in a woman with cholangiocarcinoma

Massart¹,

Lucas²,

Rioux‐Leclercq³

et al. 2005

Clinical Chemistry and Laboratory Medicine (CCLM)

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Assay of human chorionic gonadotropin (hCG) is mainly used for the detection and monitoring of pregnancy, and for the follow-up of trophoblastic tumors. The serum free beta-hCG subunit (hCGbeta) is also a tumor marker in many non-trophoblastic tumors, including gastrointestinal cancers. In this work, we compared the performance of several immunoassays for pregnancy exclusion before liver transplantation and in the follow-up of a woman with cholangiocarcinoma. Serum hCG was detected with the Abbott Testpack plus hCG-Combo and measured with four automated sandwich immunoassays: ADVIA-Centaur, ACS:180, AxSYM and Dimension. hCGbeta was determined by an automated fluorescence sandwich immunoassay (Kryptor-Free beta hCG) and with a specific immunoradiometric assay (ELSA-F beta hCG, Schering). The expression of hCG was also evaluated by immunohistochemistry on sections of intrahepatic cholangiocarcinoma cells and on peritoneal metastases. Before transplantation, discordant results were observed for pregnancy exclusion. Qualitative Testpack and Dimension tests detected no hCG-like immunoreactivity, whereas the ADVIA-Centaur, ACS:180 and AxSYM tests revealed positive levels. The same discrepancy was obtained in follow-up of the patient after liver transplantation. hCGbeta assay and immunohistochemical staining revealed tumor cell secretion of hCGbeta. In conclusion, a specific serum immunoassay for intact dimeric hCG without cross-reaction with hCGbeta should be adopted as routine policy for pregnancy exclusion before liver transplantation.

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“…The implementation of such reference systems in immunoassay, which is an important tenet of current thinking on standardization within the IFCC (3,17 ), requires both well-characterized antibodies and highly purified standards such as those described here (18 ). These developments should lead to substantial improvement in standardization and clinical utility of the next generation of assays for hCG-related molecules.…”

Section: Resultsmentioning

confidence: 99%

Establishment, Value Assignment, and Characterization of New WHO Reference Reagents for Six Molecular Forms of Human Chorionic Gonadotropin

et al. 2004

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Standardization of Assays for Human Chorionic Gonadotropin

Cited by 22 publications

References 21 publications

Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards

Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards

Importance of the detection method for intact dimeric human chorionic gonadotropin without interference with the free human chorionic gonadotropin β subunit for pregnancy exclusion before liver transplantation in a woman with cholangiocarcinoma

Establishment, Value Assignment, and Characterization of New WHO Reference Reagents for Six Molecular Forms of Human Chorionic Gonadotropin

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