Standardization of activated sludge for biodegradation tests

Vázquez‐Rodríguez, Gabriela A.; Beltrán-Hernández, Rosa Icela; Coronel-Olivares, C; Rols, Jean‐Luc

doi:10.1007/s00216-011-5212-z

Cited by 16 publications

(18 citation statements)

References 36 publications

(64 reference statements)

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“…Activated sludge is regarded as a suitable and diverse source of microorganisms, and thus is the most common inoculum source for biodegradation tests [23]. Wastewater sludge microbial communities are employed to convert a wide variety of organic substrates into biomass, bioremediation of various pollutants, and to ameliorate perturbations in the environment [33].…”

Section: Discussionmentioning

confidence: 99%

“…Wastewater sludge microbial communities are employed to convert a wide variety of organic substrates into biomass, bioremediation of various pollutants, and to ameliorate perturbations in the environment [33]. It is widely recognized that mixed cultures have a higher biodegradation potential than axenic ones, as a microbial community is able to use a wider variety of substrates because of the collective action of individual species [23]. Now, the use of activated wastewater sludge in biodegradation is thought to depend on the functional richness/diversity of such microbial communities [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…Activated sludge is a specialized, aerobically developed microbial assemblage containing flocculated and non-flocculated organisms [23]. The heterotrophic bacteria aggregated in the flocs and freely dispersed in the liquor constitute the most abundant microbial group.…”

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

“…In activated sewage sludge, the most common viable isolates are Gram-negative, non-fermentative, and oxidase-positive bacteria. Usually detected genera are Zoogloea, Pseudomonas, Achromobacter, Alcaligenes, Comamonas, and Flavobacterium [23]. Culture-dependent techniques have also revealed that bacterial diversity in activated sludge is quite different from that present in the raw influent sewage, where E. coli, Streptococcus faecalis, Salmonella typhi, and Pseudomonas aeruginosa are more abundant [23].…”

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

“…Usually detected genera are Zoogloea, Pseudomonas, Achromobacter, Alcaligenes, Comamonas, and Flavobacterium [23]. Culture-dependent techniques have also revealed that bacterial diversity in activated sludge is quite different from that present in the raw influent sewage, where E. coli, Streptococcus faecalis, Salmonella typhi, and Pseudomonas aeruginosa are more abundant [23]. As per the information provided by manufacturer, proprietary microbial inoculum used in this study consisted of a mixture of naturally occurring bacteria, fungi and actinomycetes along with enzymes.…”

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

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Evaluation of an organic soil amendment generated from municipal solid waste seeded with activated sewage sludge

Deepesh

Verma

Suma

et al. 2014

J Mater Cycles Waste Manag

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This study investigated the feasibility of using fresh activated sewage sludge as inoculum for the microbial valorization of segregated municipal solid waste and evaluated the quality of organic soil amendment generated. Organic fraction of municipal solid waste, which consisted of vegetative (vegetable, fruit and flower) wastes was seeded with activated sewage sludge and processed by rapid aerobic microbial treatment. Efficacy of microbial valorization process and quality of final product were assessed by physico-chemical analysis. Suitability of final product was assessed with regard to heavy metal content, pesticide residues, microbiological quality and phytotoxicity. Quality of the soil amendment generated was compared with the control product generated with a commercial microbial inoculum. Phytotoxicity experiments indicated the stimulatory effect of sewage sludge seeded soil amendment on plant growth but inhibition was observed in closed growth test due to the evolution of gaseous phytotoxic agents. The study suggests that segregated municipal solid waste can be effectively valorized with activated non-dewatered sewage sludge as inoculum and the quality of soil amendment generated was comparable to compost intended for unrestricted applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

Section: Microbiological Characteristics Of Sludge Inoculummentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of an organic soil amendment generated from municipal solid waste seeded with activated sewage sludge

Deepesh

Verma

Suma

et al. 2014

J Mater Cycles Waste Manag

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Synthesis, characterization and biodegradation studies of chain-coupled polyesters based on tartaric acid

et al. 2013

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A series of chain-coupled polyesters based on tartaric acid was synthesized and characterized following a two-step procedure. In the first step, tartaric acid based hydroxyl terminated polyesters with various alkane diols were prepared and then, in a second step, a chain-coupling approach using hexamethylene diisocyanate was employed on the synthesized polyesters to prepare a series of chain-coupled polyesters. The number-average molecular weights (M n ) of the polyesters were found to vary in the range (4.8 − 28.1) × 10 3 g mol −1 . Thermomechanical studies demonstrate that the storage modulus of the chain-coupled polyesters decreases with increasing polymethylene chain length which is attributable to enhanced flexibility. The isolation of bacteria on medium containing polymer as the sole source of carbon indicates the ability of the synthesized polyesters to be taken up by microorganisms for growth.

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Toward the future of OECD/ISO biodegradability testing-new approaches and developments

Strotmann

Thouand

Pagga³

et al. 2023

Appl Microbiol Biotechnol

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In the past decades, industrial and scientific communities have developed a complex standardized system (e.g., OECD, ISO, CEN) to evaluate the biodegradability of chemical substances. This system includes for OECD three levels of testing (ready and inherent biodegradability tests, simulation tests). It was adopted by many countries and is completely integrated into European legislation (registration, evaluation, authorization, and restriction of chemicals, REACH). Nevertheless, the different tests have certain deficiencies, and the question arises of how accurately these tests display the situation in the real environment and how the results can be used for predictions. This review will focus on the technical advantages and weaknesses of current tests concerning the technical setup, the inoculum characterization, and its biodegradation potential as well as the use of adequate reference compounds. A special focus of the article will be on combined test systems offering enhanced possibilities to predict biodegradation. The properties of microbial inocula are critically discussed, and a new concept concerning the biodegradation adaptation potential (BAP) of inocula is proposed. Furthermore, a probability model and different in silico QSAR (quantitative structure-activity relationships) models to predict biodegradation from chemical structures are reviewed. Another focus lies on the biodegradation of difficult single compounds and mixtures of chemicals like UVCBs (unknown or variable composition, complex reaction products, or biological materials) which will be an important challenge for the forthcoming decades. Key points • There are many technical points to be improved in OECD/ISO biodegradation tests • The proper characterization of inocula is a crucial point in biodegradation tests • Combined biodegradation test systems offer extended possibilities for biodegradation tests

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Standardization of activated sludge for biodegradation tests

Cited by 16 publications

References 36 publications

Evaluation of an organic soil amendment generated from municipal solid waste seeded with activated sewage sludge

Evaluation of an organic soil amendment generated from municipal solid waste seeded with activated sewage sludge

Synthesis, characterization and biodegradation studies of chain-coupled polyesters based on tartaric acid

Toward the future of OECD/ISO biodegradability testing-new approaches and developments

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