Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population

Rajendran, Priya; Baskaran, Mani; Balaji, Sarath; Shanmugam, Sivakumar; Palanisamy, Sivaraman; Ramamoorthy, Thirumalani; Hasini, Sindhu; Devaleenal, Bella; Watson, Basilea

doi:10.4103/ijmy.ijmy_126_22

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…Strong involvement of not only the different health institutions, but also the affected families is needed to promote TB prevention and protect exposed children. 16 Our results also seek to foster governmental commitment to TB control [17][18][19][20] as a public health priority and feasible strategies to achieve it.…”

Section: Discussionmentioning

confidence: 96%

Overview, Trends and Mapping of The Scientific Production on Childhood Tuberculosis: A Scientometric Study

Espinoza-Carhuancho,

Medina,

Mauricio-Vilchez

et al. 2024

Natl J Community Med

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Background: Tuberculosis in children is highly prevalent and is considered community transmission by an infected adult. This study analyzed childhood tuberculosis literature from 2018 to 2023 on Scopus. Methodology: The study was an observational, retrospective design with a bibliometric approach focusing on the scientific production on childhood tuberculosis. A trend analysis and mapping of the literature published in Scopus was performed. The following selection criteria were applied: All types of papers published in Scopus during the study period were included. Papers published between 2018 and 2023 were included. Papers had to be focused on childhood tuberculosis. Results: Antoni Diez Noguera-Julián from SJD Barcelona Children’s Hospital had a field-weighted citation impact of 10.89. Anneke Catharina Hesseling and James Alexander Seddon had the highest output. The University of Melbourne and the University of Barcelona had high field-weighted citation impacts. Despite the United States’ high production, Spain and Australia showed significant influence. The “International Journal of Tuberculosis and Lung Disease” led in production. Conclusions: A bibliometric study on childhood tuberculosis literature from 2018-2023 highlighted Antoni Diez Noguera-Julián’s significant citation impact and the high output of Anneke Catharina Hesseling and James Alexander Seddon. Despite the U.S.'s high production, Spain and Australia showed significant influence.

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Section: Discussionmentioning

confidence: 96%

Overview, Trends and Mapping of The Scientific Production on Childhood Tuberculosis: A Scientometric Study

Espinoza-Carhuancho,

Medina,

Mauricio-Vilchez

et al. 2024

Natl J Community Med

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“…For the other TST-positive children, although they did not turn out to be positive by any MTB diagnostic test and had normal chest x-rays (except one), the symptoms listed out and the presence of other bacterial pathogens implicate the need for further research. The symptoms could be either due to bacterial pathogens identified mimicking TB or MTB itself that could not be detected owing to their paucibacillary status in children ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Paediatric pulmonary disease—are we diagnosing it right?

Rajendran,

Thomas,

Balaji

et al. 2024

Front. Pediatr.

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BackgroundIt has been reported that differential diagnosis of bacterial or viral pneumonia and tuberculosis (TB) in infants and young children is complex. This could be due to the difficulty in microbiological confirmation in this age group. In this study, we aimed to assess the utility of a real-time multiplex PCR for diagnosis of respiratory pathogens in children with pulmonary TB.MethodsA total of 185 respiratory samples [bronchoalveolar lavage (15), gastric aspirates (98), induced sputum (21), and sputum (51)] from children aged 3–12 years, attending tertiary care hospitals, Chennai, India, were included in the study. The samples were processed by N acetyl L cysteine (NALC) NAOH treatment and subjected to microbiological investigations for Mycobacterium tuberculosis (MTB) diagnosis that involved smear microscopy, Xpert® MTB/RIF testing, and liquid culture. In addition, DNA extraction from the processed sputum was carried out and was subjected to a multiplex real-time PCR comprising a panel of bacterial and fungal pathogens.ResultsOut of the 185 samples tested, a total of 20 samples were positive for MTB by either one or more identification methods (smear, culture, and GeneXpert). Out of these 20 MTB-positive samples, 15 were positive for one or more bacterial or fungal pathogens, with different cycle threshold values. Among patients with negative MTB test results (n = 165), 145 (87%) tested positive for one or more than one bacterial or fungal pathogens.ConclusionThe results suggest that tuberculosis could coexist with other respiratory pathogens causing pneumonia. However, a large-scale prospective study from different geographical settings that uses such simultaneous detection methods for diagnosis of childhood tuberculosis and pneumonia will help in assessing the utility of these tests in rapid diagnosis of respiratory infections.

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“…In recent years, frequently encountered NTM species in postsurgical wound infections are M. chelonae and M. fortuitum. 10 NTM are transmitted through aerosol, soil, dust, or contaminated tap water. 11 In our study, all the postoperative wounds were healed initially within 7-10 days of surgery.…”

Section: Discussionmentioning

confidence: 99%

Postoperative wound infection by nontuberculous mycobacteria; case series in Dhaka Medical College Hospital of Bangladesh

Halder,

Tanni,

Kabir

et al. 2023

Clinical Case Reports

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Key Clinical MessageThe incidence of nontuberculous mycobacterial (NTM) infections after operations is increasing in Bangladesh but data regarding clinical presentation, diagnosis, treatment, and prognosis after treatment are lacking. In this case series, three patients having persistent serous discharge from incision wound after operation were studied. Discharge from wounds were collected, wet film microscopy was performed for pus cells and fungus, Gram stain, Ziehl‐Neelsen (ZN) stain, culture in routine culture media and Lowenstein‐Jensen (LJ) media, Xene‐Xpert for mycobacterium tuberculosis (MTB), polymerase chain reaction (PCR) for NTM were done. NTM‐positive patients were treated initially for 6 weeks with four drugs regimen (clarithromycin 500 mg 12 hourly, ciprofloxacin 500 mg 12 hourly, linezolid 400 mg 12 hourly, and amikacin 500 mg 12 hourly), followed by 5 months with three drugs regimen (clarithromycin 500 mg 12 hourly, ciprofloxacin 500 mg 12 hourly, and linezolid 400 mg 12 hourly) as a maintenance dose. Cessation of discharge occurred within 3–4 weeks after starting treatment, and the wounds were healed.

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Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population

Cited by 5 publications

References 25 publications

Overview, Trends and Mapping of The Scientific Production on Childhood Tuberculosis: A Scientometric Study

Overview, Trends and Mapping of The Scientific Production on Childhood Tuberculosis: A Scientometric Study

Paediatric pulmonary disease—are we diagnosing it right?

Postoperative wound infection by nontuberculous mycobacteria; case series in Dhaka Medical College Hospital of Bangladesh

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