Standardization of a Patella spp. (Mollusca, Gastropoda) embryo–larval bioassay and advantages of its use in marine ecotoxicology

Pérez, Sara; Fernández, Narciso de Gabriel; Ribeiro, Pedro A.

doi:10.1016/j.ecoenv.2016.01.025

Cited by 17 publications

(17 citation statements)

References 27 publications

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“…These limpets, which are ecologically important in coastal regions, separated from other gastropods early in molluscan evolution 5 . They can be used in marine ecotoxicology research because of their wide distribution, and their gametes are available throughout the year 6 . This group is characterized by intraspecific polyphenism, the existence of cryptic species, and the intraspecific variation of characteristics such as shell morphology and colour.…”

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confidence: 99%

Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda

Jiao

Guo

Yan

et al. 2020

Sci Rep

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To improve the systematics and taxonomy of Patellogastropoda within the evolution of gastropods, we determined the complete mitochondrial genome sequences of Lottia goshimai and Nipponacmea fuscoviridis in the family Lottiidae, which presented sizes of 18,192 bp and 18,720 bp, respectively. In addition to 37 common genes among metazoa, we observed duplication of the trnM gene in L. goshimai and the trnM and trnW genes in N. fuscoviridis. The highest A + T contents of the two species were found within protein-coding genes (59.95% and 54.55%), followed by rRNAs (56.50% and 52.44%) and tRNAs (56.42% and 52.41%). trnS1 and trnS2 could not form the canonical cloverleaf secondary structure due to the lack of a dihydrouracil arm in both species. The gene arrangements in all Patellogastropoda compared with those of ancestral gastropods showed different levels of gene rearrangement, including the shuffling, translocation and inversion of single genes or gene fragments. This kind of irregular rearrangement is particularly obvious in the Lottiidae family. The results of phylogenetic and gene rearrangement analyses showed that L. goshimai and Lottia digitalis clustered into one group, which in turn clustered with N. fuscoviridis in Patellogastropoda. This study demonstrates the significance of complete mitogenomes for phylogenetic analysis and enhances our understanding of the evolution of Patellogastropoda.

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confidence: 99%

Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda

Jiao

Guo

Yan

et al. 2020

Sci Rep

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“…Recently, Pérez et al . (2016) standardized a Patella spp. embryo-larval bioassay for use in ecotoxicological studies, which included data on P. ulyssiponensis , providing reliable protocols for larval rearing.…”

Section: Discussionmentioning

confidence: 99%

“…However, the endpoint established in Pérez et al . (2016) was the percentage of normal trochophore larvae and therefore it would be important to apply the same approach to the latter stages of larval development. In contrast with Hodgson et al .…”

Section: Discussionmentioning

confidence: 99%

“…(2007), it is possible to compare our results to those of Pérez et al . (2016) for rearing P. ulyssiponensis up to the trochophore stage because they assessed survival in the same way (percentage of normal trochophores relative to the initial number of fertilized eggs) and used the same incubation temperature (18°C). Our fertilization success was low (24%), although within the range of 21.6–46.3% obtained by Pérez et al .…”

Section: Discussionmentioning

confidence: 99%

“…Rearing of larval patellids to settlement and metamorphosis is also very rare (but see Smith, 1935; Dodd, 1957; Wanninger et al ., 1999; Ferranti et al ., 2018). Laboratory assays with Patella embryos and larvae are often referred to as methodologically difficult (Dodd, 1957; Smaldon & Duffus, 1984; Espinosa et al ., 2010; Pérez et al ., 2016), which has probably impaired the progress of laboratory settlement studies.…”

Section: Introductionmentioning

confidence: 99%

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Recruitment of the limpetPatella ulyssiponensisand its relationship with crustose coralline algae: patterns of juvenile distribution and larval settlement

et al. 2019

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Recruitment of the limpet Patella ulyssiponensis was investigated in relation to the presence of living crustose coralline algae (CCA) in rocky-shore habitats. Juvenile limpets (≤10 mm maximum shell length) were counted in CCA-present and CCA-absent habitats, on three shores in SW Portugal during summer 2007 and winter 2009. Furthermore, the settling response of laboratory-reared larvae of P. ulyssiponensis to CCA-covered substratum, and bare-rock, was examined. Across the intertidal zone, we found a clear association between the distribution and abundance of juveniles and the presence of CCA. Although the presence of CCA was not an absolute requisite for juvenile occurrence, null juvenile densities were mostly recorded in CCA-absent areas. The highest juvenile densities (maximum of 64 individuals in 15 × 15 cm) were consistently found in CCA-dominated habitats, namely steep wave-exposed areas at low-shore and rock-pools. The hypothesis of CCA-enhanced settlement was not supported, as settlement intensities of laboratory-reared larvae were similar between chips of rock encrusted by CCA and chips of bare-rock. From the overall number of settlers onto CCA-encrusted rock chips, 51% were found in tiny pits lacking CCA. This was the first study of the settlement patterns of larvae of the genus Patella using naturally occurring rocky substrata. These results are preliminary and should be confirmed with choice-experiments and improved monitoring of the position of settlers. We suggest that CCA plays a role in the recruitment of P. ulyssiponensis, potentially promoting survivorship of early benthic stages, but possibly not enhancing settlement.

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Hatchery practices for Patella aspera and P. ordinaria: evaluating culture vessels requirements

Castejón,

Sousa,

Andrade

2024

Aquacult Int

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Innovation in limpet production requires knowledge of the optimal qualities and limitations of the culture vessels. Two limpet species from Madeira fishery, Patella aspera and P. ordinaria, were used to evaluate the influence of culture vessel shape (microtubes, test tubes, centrifuge tubes, specimen cups, disposable cups, vials, flasks and beakers), composition (polypropylene and glass), volume (1.5 ml to 1.5 L) and stocking density on oocyte incubation and larval culture through nine assays. Moreover, three assays evaluated the use of microtubes and the isolation of trochophores, instead of pediveligers, for eventual settlement induction. The results showed effective hatching in most culture vessels regardless of composition or volume, except when the shape of the bottom caused oocyte clustering. Larval culture was not influenced by any variable under scrutiny (shape, composition, volume or rearing density). Microtubes were useful for settlement, while the placement of trochophores in the settling vessels, instead of pediveligers, proved inefficient and even led to failed cultures. The best settlement was obtained when the pediveligers were isolated from the water column of the beakers and transferred to clean vessels with renewed water and coralline algae as substrate. The current findings could be extrapolated to other marine invertebrates that share similar life stage requirements.

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Standardization of a Patella spp. (Mollusca, Gastropoda) embryo–larval bioassay and advantages of its use in marine ecotoxicology

Cited by 17 publications

References 27 publications

Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda

Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda

Recruitment of the limpetPatella ulyssiponensisand its relationship with crustose coralline algae: patterns of juvenile distribution and larval settlement

Hatchery practices for Patella aspera and P. ordinaria: evaluating culture vessels requirements

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