Standardization of a new method for assessing the development of cataract in cultured bovine lenses

Heruye, Segewkal; Maffofou, N N Leonce; Singh, Neetu; Munt, Dan; Njie‐Mbye, Ya Fatou; Ohia, Sunny E.; Opere, Catherine A.

doi:10.1016/j.vascn.2019.106592

Cited by 6 publications

(7 citation statements)

References 39 publications

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“…Indeed, at the highest concentrations tested, both H 2 Sreleasing compounds did not protect lenses from time-dependent loss in transparency. Interestingly, lower concentrations (10 -6 M and 10 -5 M) of L-cysteine, the substrate for endogenous biosynthesis of H 2 S were more potent at preventing cataract formation in cultured bovine lenses than that exhibited by higher concentrations (10 -3 M) of the amino acid [24]. The increased ability of low concentrations of DATS and GYY4137 to prevent time-dependent loss of lens opacity may be due to several factors such as the rate of H 2 S release from these compounds, physical/chemical characteristics of the agents (e.g hydrophobicity and /or hydrophilicity), and the presence of transporters.…”

Section: T I M E ( H O U R S ) T R a S M It T A N C E ( 4 2 0 N M )mentioning

confidence: 93%

“…Transmittance was measured at 420 nm using a plate reader (Synergy H1 hybrid reader; Bio Tek Instruments, Inc) at zero, three, and six hours after culture on the first day followed by 24 hours measurements up to 120 hours. There is evidence that maximum decrease in transmittance is achieved at the 420 nm wavelength, rendering it as an acceptable indicator for the development of opacification in cultured bovine lenses [24]. No transmittance change from initial measurement (0 hour) or an increase in transmittance compared to untreated lenses at 120 hours was taken as prevention of time-dependent loss of lens transparency.…”

Section: Assessment Of Opacitymentioning

confidence: 99%

“…Since oxidative stress has been reported to play a major role in the pathogenesis of age-related cataract, several studies have been aimed at mitigating cataract formation by using antioxidants to target various antioxidant systems [44][45][46][47][48][49][50]. Mechanisms reported to account for the antioxidant activity of H 2 S include free radical scavenging reaction [51], increase in GSH content [24,52] and SOD activity [24]. Therefore, in this study, we further examined the role of SOD and GSH on H 2 S-mediated protection from time-dependent lens opacification in bovine lenses, ex vivo.…”

Section: T I M E ( H O U R S ) T R a S M It T A N C E ( 4 2 0 N M )mentioning

confidence: 99%

“…Furthermore, inhibition, proteolytic degradation, and downregulation of CSE resulted in lenticular GSH depletion and increased risk of cataract formation, suggesting a significant physiological role of H 2 S in the eye [22][23]. In cultured bovine lenses, the substrate for endogenous production of H 2 S, L-cysteine attenuated time-dependent cataract formation with a corresponding decline in GSH content and SOD enzyme activity [24]. Accordingly, we designed experiments in the present study using lenses that were cultured ex vivo to observe the time-dependent loss of lens transparency.…”

Section: Introductionmentioning

confidence: 98%

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Regulation of time-dependent lens opacification by hydrogen sulfide-releasing compounds

Heruye¹,

Mbye²,

Ohia³

et al. 2021

New Front Ophthalmol

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Purpose: Although cataract is the leading cause of blindness worldwide, there are no pharmacological agents to prevent or reverse the disease. Since oxidative stress is implicated in the pathogenesis of cataracts, and hydrogen sulfide (H 2 S) exhibits antioxidant and neuroprotective properties, the primary objective of this study was to investigate the ability of H 2 S-releasing compounds to prevent time-dependent loss of transparency in the cultured bovine lens. Methods:Freshly isolated bovine lenses were cultured in DMEM containing H 2 S-producing compounds, diallyl trisulfide (DATS), GYY4137 or ascorbic acid (AA; used as positive control). Lens opacity was determined by measuring transmittance (using Synergy H1 hybrid reader) and by visual inspection of cultured lenses up to 120 h. Total glutathione (GSH) content and superoxide dismutase (SOD) activity were determined in lens homogenates using Cayman Chemical Assay kits.Results: After 120 hours of incubation, DATS (10 -7 and 10 -6 M) significantly (p<0.001; n=6) attenuated the time-dependent decline in transmittance by 16.91 ± 0.01% and 28.54 ± 0.15%, while GYY4137 (10 -7 and 10 -6 M) also reduced transmittance by 22.05 ± 0.02% and 2.45 ± 0.32%, respectively. Similarly, DATS (10 -7 M and 10 -6 M) and GYY4137 (10 -7 M and 10 -6 M)-treated lenses exhibited relatively clear grids after 120 hours, compared to control and AA (10 mM)-treated lenses. DATS (10 -6 M) and GYY4137 (10 -7 M) significantly (p<0.001; n=5) reversed time-dependent decline in total GSH content by 69.69 ± 0.01% and 80.52 ± 0.17% and SOD activity by 3.23 ± 2.05% and 19.31 ± 0.89%, respectively. Conclusion:H 2 S-releasing compounds prevented time-dependent cultured lens opacification by an action that depends, at least in part, on the integrity of the antioxidant defense pathways, ex vivo.

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Section: T I M E ( H O U R S ) T R a S M It T A N C E ( 4 2 0 N M )mentioning

confidence: 93%

Section: Assessment Of Opacitymentioning

confidence: 99%

Section: T I M E ( H O U R S ) T R a S M It T A N C E ( 4 2 0 N M )mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Regulation of time-dependent lens opacification by hydrogen sulfide-releasing compounds

Heruye¹,

Mbye²,

Ohia³

et al. 2021

New Front Ophthalmol

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show abstract

“…Moreover, novel studies in microRNA´s played a fundamental role in the pathogenesis not only in cataracts but in the Ophthalmology discipline, etiologist involved like macular degeneration, retinoblastoma, glaucoma and pterygium for mentioning some, the non coding molecules have specific participation down-regulating translational processes, like apoptosis cell proliferation and differentiation; one of the most relevant is MIR-182-5p, mainly due interaction in LEC, delaying apoptosis with NOX4 (nicotinamide adenine dinucleotide phosphate oxidase subunit 4) directly responsible in ROS source formation, p38 protein and MAPK (mitogen-activated protein kinase) pathway [53][54][55][56][57].…”

Section: Fig 2 Schematic Representation Of Trimetazidine (Tmz) Preven...mentioning

confidence: 99%

Reactive Oxygen Species and Eye Aging in Cataracts through Biomolecular Mechanisms

Pacheco

2022

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The cataractogenesis process and the novel mechanisms involved in its progression make us question that the only solution today presented for its resolution is through a surgical procedure. The unveiling of such interesting and intricate machinery in the lens opacification occupies not only the ophthalmologists field, but also biology, molecular, and biochemistry areas. The animal models, experimental and theoretically, have done their best try to present what appears to be an irreparable and unstoppable process nowadays. The crystalline lens opacification is chronic and progressively damaged due to the loss of balance between the production and elimination of free reactive oxygen species (ROS), the attempts to stop or slow down this overproduction or lack of elimination lie in study material from disciplines as complex as cancer pathways. The execution of this review article was possible through metasearch engines like Wiley Online Library, EBSCO, Cochrane, Clinical key and MEDLINE PubMed (National Library of Medicine, National Institutes of Health), to retrieve studies from January 2021 to January 2022, following PRISMA guidelines, with a special affinity to systematic reviews and meta-analysis. MeSH Terms associated with "Cataract AND Reactive Oxygen Species". This work constitutes an integral cataractogenesis view in the development of future therapeutic scopes. The world population especially in Europe is aging at a rapid rate, in this sense is necessary to consider a root potential solution and not palliative strategies in ophthalmology for the next generations. The compendium of procedures presented already supposed to open a new panorama to a clearer world, a world without senile cataracts. Time is vision.

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Small molecule inhibitor of TAK1 ameliorates rat cartilaginous endplate degeneration induced by oxidative stress in vitro and in vivo

Tang¹,

Chen

Gao

et al. 2020

Free Radical Biology and Medicine

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Standardization of a new method for assessing the development of cataract in cultured bovine lenses

Cited by 6 publications

References 39 publications

Regulation of time-dependent lens opacification by hydrogen sulfide-releasing compounds

Regulation of time-dependent lens opacification by hydrogen sulfide-releasing compounds

Reactive Oxygen Species and Eye Aging in Cataracts through Biomolecular Mechanisms

Small molecule inhibitor of TAK1 ameliorates rat cartilaginous endplate degeneration induced by oxidative stress in vitro and in vivo

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