Standardisation of methods for assessing mould germination: A workshop report

Abstract

The first workshop on predictive mycology was held in Marseille, France, 2--4 February 2005 under the auspices of the French Microbiological society. The purpose of the workshop was to list the different techniques and definitions used by scientists for assessing mould germination and to evaluate the influence of the different techniques on the experimental results. Recommendations were made when a large consensus was obtained. In order to facilitate the study of germination, alternative methods to microscopic examination were examined.

Evaluation of chlorine dioxide as an antimicrobial against Botrytis cinerea in California strawberries

“…Therefore, to improve the quality of fruits and vegetables, and to help prolong their shelf life, it is necessary to both understand the behavior of fungal growth and to identify methods that effectively reduce the population of microorganisms. Researchers have proposed models to describe microorganism population growth as a function of time, and to simulate and predict the behavior of different types of fungi growth at different environmental conditions and at different growth stages (germination and hyphae extension) such as logistic, the Gompertz and the Baranyi models (Dantigny et al, 2006(Dantigny et al, , 2007Declerck et al, 2001;López et al, 2004).…”

Actividad antifúngica in vitro del aceite esencial de ajo (Allium sativum L.) contra Alternaria tenuissima

“…Las cajas de Petri se incubaron a 25 °C con fotoperiodo de 12 h luz-oscuridad hasta un tiempo máximo de 24 h. Se tomaron muestras aleatorias cada 4 h y se contó el número de esporas germinadas y no germinadas de un total de 200 esporas bajo un microscopio óptico. Se consideró como una espora germinada cuando la longitud del tubo germinativo fue de al menos el 50% de la longitud de la espora sin germinar (Dantigny et al, 2006). conditions as described above.…”

“…The Petri dishes were incubated at 25 ºC with a photoperiod of 12 h light-darkness for up to 24 h. Random samples of each were taken every 4 h and the number of germinated and not germinated spores were counted, out of a total 200 spores, under a light microscope. The spore was considered germinated when the length of the germinative tube was at least 50% as long as the not germinated spore (Dantigny et al, 2006). Experimental Design and Statistical Analysis.…”

Modeling red cabbage seed extract effect on Penicillium corylophilum: Relationship between germination time, individual and population lag time

“…A spore suspension was then prepared by washing the culture with sterile distilled water containing 0.01% (vol/vol) Tween® 80 (Merck KGaA, Darmstadt, Germany) (Dantigny et al, 2006). After enumeration using a Malassez counting chamber, the suspension was adjusted to 10 6 spores/ml and was used for inoculation as quickly as possible (less than a few minutes).…”