Standardisation de la classification morphologique des spermatozoïdes humains selon la méthode de David modifiée

Auger, Jacques; Eustache, Florence; David, G

doi:10.1007/bf03034491

Cited by 76 publications

(81 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By reason of modifications in this classification, the evolution of 'normal sperm morphology' and of 'bent tail' is presented until the end of 2002. For the neck, midpiece and tail, the abnormalities described by Auger and Eustache, 32 'asymetrical insertion', 'thin midpiece' and 'irregular tail' did not appear in the David classification. The other abnormalities were studied over the 20 years of the study.…”

Section: Semen Analysismentioning

confidence: 76%

“…Since the opening of the laboratory, the strictest criteria have been applied. All semen samples wereanalysed according to standard methods, [29][30][31][32] and efforts were made to keep the technique of semen analysis unchanged. Only the parameters which did not change over time were taken into account.…”

Section: Discussionmentioning

confidence: 99%

“…Normal sperm morphology and bent tails were studied until the end of 2002. Indeed, Auger and Eustache classification, 32 which was applied in our laboratory since 2003, introduced modifications of these two parameters. Decline of semen quality among males consulting for couple infertility C Geoffroy-Siraudin et al 587…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

Geoffroy-Siraudin¹,

Loundou²,

Romain³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

Semen from 10 932 male partners of infertile couples was analysed and sperm parameter trends were evaluated at the Reproduction Biology Laboratory of the University Hospital of Marseille (France) between 1988 and 2007. After 3-6 days of abstinence, semen samples were collected. Measurements of seminal fluid volume, pH, sperm concentration, total sperm count, motility and detailed morphology of spermatozoa were performed. Sperm parameters were analysed on the entire population and in men with normal total numeration (o40 million per ejaculate). The whole population demonstrated declining trends in sperm concentration (1.5% per year), total sperm count (1.6% per year), total motility (0.4% per year), rapid motility (5.5% per year) and normal morphology (2.2% per year). In the group of selected samples with total normal sperm count, the same trends of sperm quality deterioration with time were observed. Our results clearly indicate that the quality of semen decreased in this population over the study period. Keywords: environment; male infertility; regional differences; semen quality; sperm parameters INTRODUCTION Numerous studies suggest a decline in semen quality in some parts of the world. 1-8 By contrast, other studies have shown no evidence of deteriorating semen quality. 9-16 Some studies concern infertile couples, while others analyse normal sperm parameters. Thus, a worldwide decreasing trend in semen parameter values can be neither confirmed nor rejected. 17 It was recently reported that fecundity is affected by this decrease. 18,19 It was speculated that decreasing sperm parameters are in response to adverse environmental factors. Nevertheless, the observed discrepancies between studies suggest regional differences in semen quality. [20][21][22] Variations in environmental factors, industrial pollution and/or lifestyle could explain these discrepancies. A large number of environmental factors can pollute air, drinking water and food. This can directly alter the male gonad by blood-testis barrier disruption and cellular toxicity, or disrupt androgen biosynthesis and action. Among these environmental factors, oestrogenic or anti-androgenic endocrine disrupting compounds inhibit critical cellular process controlling steroidogenesis in Leydig cells and androgen binding to the androgen receptor. 23 It has been hypothesized that endocrine disrupting compounds play a major role in the aetiology of increased incidence of testicular dysgenesis syndrome which gathers decreased quality of semen, cryptorchidism, hypospadias and testicular cancer. 24 Chemicals with hormone-like activity can also

show abstract

Section: Semen Analysismentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

Geoffroy-Siraudin¹,

Loundou²,

Romain³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…Another potential weakness of our study is that sperm morphology was assessed using the David classification rather than strict (Tygerberg) criteria. However, we believe that the application of the 4 % thresholds (the 5th percentile from the new WHO reference range) is appropriate given that the new WHO semen analysis morphology reference values were derived in large part from studies using the David morphology classification [23,[25][26][27].…”

Section: Discussionmentioning

confidence: 99%

“…After liquefaction of semen, the semen analysis was performed according to the World Health Organization (WHO) guidelines and included assessment of semen volume, sperm concentration, % progressive motility. The % normal morphology was assessed according to the modified David classification after Harris-Schorr staining [23][24][25]. The David classification of morphological anomalies of human spermatozoa was developed in the 1970s and modified in the early 1990s to account for all of the defects known to interfere with normal sperm functions.…”

Section: Semen Analysismentioning

confidence: 99%

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Belloc¹,

Benkhalifa²,

Cohen-Bacrie³

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Background Sperm DNA damage is common in infertile men and is associated with poor semen parameters but the impact of an isolated sperm abnormality on sperm DNA damage has not been studied. Objective To evaluate sperm DNA damage in a large cohort of infertile men with isolated sperm defects. Design, setting and participants Retrospective study of 1084 consecutive, non-azoospermic infertile men with an isolated sperm defect: isolated oligozoospermia (iOligo), isolated asthenozoospermia (iAstheno) or isolated teratozoospermia (iTerato). Outcome measurements and statistical analysis We examined and compared clinical parameters, conventional semen parameters and %sperm DNA fragmentation (%SDF, assessed by flow cytometry-based Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling assay) in the three groups of men. Results and limitations The mean (±SD) %SDF was significantly higher in the iAstheno compared to the iOligo and iTerato groups (25.0±14.0 vs. 19.2±11.6 and 20.7±12.1 %, respectively, P<0.0001). Similarly, the proportion of men with high %SDF (>30 %) was significantly higher in the iAstheno compared to the iOligo and iTerato groups (31 % vs. 18 % and 19 %, respectively, P<0.0001). In the group of 713 men with iAstheno, %SDF was positively correlated with paternal age (r=0.20, P<0.0001) and inversely correlated with %progressive motility (r=−0.18, P<0.0001). In the subset of 218 men with iTerato, %SDF was also positively correlated with paternal age (r=0.15, P=0.018) and inversely correlated with %progressive motility (r=−0.26, P=0.0001). Conclusions In this large cohort of infertile men with isolated sperm abnormalities, we have found that the sperm DNA fragmentation level is highest in the men with sperm motility defects and that 31 % of these men have high levels of sperm DNA fragmentation. The data indicate that poor motility is the sperm parameter abnormality most closely related to sperm DNA damage.

show abstract

Effets in vitro des dermaseptines sur les caractéristiques du sperme

Ajina¹,

Zaïri

Mougou³

et al. 2005

Androl.

View full text Add to dashboard Cite

show abstract

Standardisation de la classification morphologique des spermatozoïdes humains selon la méthode de David modifiée

Cited by 76 publications

References 15 publications

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Effets in vitro des dermaseptines sur les caractéristiques du sperme

Contact Info

Product

Resources

About