Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns

Cliquet, Florence; Müller, Thomas; Mutinelli, Franco; Geronutti, Sabrina; Brochier, Bernard; Selhorst, Thomas; Schereffer, J.L.; Krafft, Nicolas; Burow, J; Schameitat, Astrid; Schlüter, Hartmut; Aubert, Michel

doi:10.1016/s0264-410x(03)00102-6

Cited by 32 publications

(19 citation statements)

References 10 publications

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“…7). These findings correlated well with the observation as performed earlier with ELISA using Glycoprotein antigen taking positive and negative control serum for standardization and establishment of a rabies test for assessing the efficacy of oral fox vaccination campaigns [5]. Capture ELISA based on monoclonal antibodies to rabies nucleoprotein (N) and glycoprotein (G) was developed to detect immune complexes to rabies N and G proteins [17].…”

Section: Discussionsupporting

confidence: 84%

Development of Monoclonal Antibodies Suitable for Rabies Virus Antibody and Antigen Detection

2012

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The control of an infectious viral disease as rabies is made easier by rapid and accurate diagnosis. Successful rabies prophylaxis is dependent upon the active immunization with vaccine along with passive administration of rabies virus neutralizing antibodies which together clear the virus before widespread infection of central nervous system occurs. The present study aimed at the development of monoclonal antibodies (MAbs) suitable for rabies virus antibody and antigen detection. For the production of rabies specific MAbs, immunization of Swiss albino mice with a commercially available vaccine was done and Polyethylene glycol mediated fusion of spleenocytes with myeloma cells was performed. The positive clones were selected on the basis of distinct reactivity by cell Enzyme linked immunosorbent assay and fluorescence in Indirect Fluorescent antibody test. The positive clones obtained were subjected to single cell cloning by limiting dilution method. The reactive clones were further titrated and employed for virus titration and virus neutralization. The neutralizing activity was evaluated using Fluorescence Activated Cell Sorter technique. Three MAb clones showed a distinct percent inhibition in the presence of positive serum. One of the MAb clone No. 5C3 was relatively more specific in detecting rabies antibodies and also found suitable for competitive ELISA to assess the antibody level in vaccinated subjects.

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Section: Discussionsupporting

confidence: 84%

Development of Monoclonal Antibodies Suitable for Rabies Virus Antibody and Antigen Detection

2012

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“…In recent years, multiple ELISA techniques have been described for quantification of rabies antibodies (Cliquet et al 2003). Even though they are quick and simple, their disadvantage is that they detect almost all IgG antibodies that bind to the antigen-coated plates, and not only the actual anti-rabies neutralizing antibodies.…”

Section: Discussionmentioning

confidence: 99%

Detection of rabies antibodies in dog sera

Ondrejková

Süli

Ondrejka

et al. 2015

Polish Journal of Veterinary Sciences

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In the presented work, we compared the results of determination of rabies antibodies using three in vitro methods: rapid fluorescent focus inhibition test (RFFIT), fluorescent antibody virus neutralisation test (FAVNT) and the immunoenzymatic assay (ELISA). 196 dog sera samples were examined with FAVNT, RFFIT methods and the ELISA test. Sera with low and sufficiently high titre of antibodies had a similar result in determining by all methods. A critical level of rabies antibodies close to the required protection level (0.5 IU/cm 3 ) was seen in sera of 18 dogs (9.18%); these were the sera obtained after primary vaccination of dogs. At this level, even small differences can cause a change in the assessment of the patient's serum seronegativity or seropositivity. Therefore, it is important to choose the appropriate method that has sufficiently strict criteria while having a good reproducibility.

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“…The following controls were used -NC R3 (non-reactive TRIS-EDTA), PC R4a (0.5 EU/ml canine serum with anti-rabies IgG), PC R4b (4 EU/ml canine serum with antirabies IgG). Samples with a titre > 0.5 EU/ml were considered positive/seroconverted (Cliquet et al 2003).…”

Section: Methodsmentioning

confidence: 99%

“…Traditionally, recommended serological methods include the rapid fluorescent foci inhibition test (RFFIT) (Smith et al 1996) and the fluorescent antibody virus neutralisation test (FAVN) (Cliquet et al 1998). In addition, several enzyme-linked immunosorbent assays (ELISA) have also been developed to detect rabies-specific antibodies in animals, and most of them have been validated for their applicability with wildlife samples (Cliquet et al 2003;Servat et al 2008;De Benedictis et al 2012). In 2010, eight out of the 11 EU countries implementing ORV programs adopted an ELISA method to monitor vaccine efficacy, with two using RFFIT and one using FAVN as gold-standards for comparison (De Benedictis et al 2012).…”

mentioning

confidence: 99%

The field efficiency of oral rabies vaccination in the Lithuanian red fox population from 2006 to 2013

Zienius¹,

Pridotkas²,

Jacevičienė³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:The objective of the present study was to assess the efficacy of an oral rabies vaccination (ORV) program in the period from 2006 to 2013 based on epidemiological data (Incidence of confirmed rabies in red foxes), biological marker evaluation (presence of tetracycline [TTC] indicating bait uptake), and the assessment of seroconversion (based on ELISA for rabies antibodies) in red foxes in Lithuania. Results were compared between juvenile and adult red foxes, and between the spring (March to May) and autumn (October to December) campaigns. On average, 20 baits/km 2 were distributed over the entire territory of Lithuania. During the entire period

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Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns

Cited by 32 publications

References 10 publications

Development of Monoclonal Antibodies Suitable for Rabies Virus Antibody and Antigen Detection

Development of Monoclonal Antibodies Suitable for Rabies Virus Antibody and Antigen Detection

Detection of rabies antibodies in dog sera

The field efficiency of oral rabies vaccination in the Lithuanian red fox population from 2006 to 2013

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