Standard operating procedure reduces interoperator variation and improves accuracy when measuring packed cell volume

Breheny, Craig; Salgado, Jorge Perez-Accino; Bommer, Nick; Handel, Ian; Gow, Adam

doi:10.1136/vr.104774

Cited by 7 publications

(10 citation statements)

References 5 publications

(12 reference statements)

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“…This data is not available. The value used for PCV was taken from a study using canine samples, 11 whereas the EPOC value was taken from a study on horses, 10 and the CV for the Abaxis was calculated from internal quality control measurements. Since horses are the closest domestic relatives to rhinoceroses, 14 values from equine hematology studies were preferred.…”

Section: Discussionmentioning

confidence: 99%

“…The coefficient of variation (CV, analytical imprecision) was calculated for the Abaxis (CV A ) from results of internal quality control and was 5%. A CV of 4.3% was used for the EPOC (CV E ) 10 and 4.0% for the manual PCV method (CV MM ) 11 . Limits of agreement derived from these CVs were calculated in MS Excel using the following formulae 8,12 :

CIP upper and lower limits for EPOC ()(%) goodbreak= 0 goodbreak\pm 1.96 goodbreak\times \sqrt{{CV}_{E}^{2} + {CV}_{MM}^{2}}

CIP upper and lower limits for Abaxis ()(%) goodbreak= 0 goodbreak\pm 1.96 goodbreak\times \sqrt{{CV}_{A}^{2} + {CV}_{MM}^{2}}

The mean difference (absolute and percentage) for each paired result was calculated (manual PCV vs EPOC; manual PCV vs Abaxis), and upper and lower limits of acceptance were generated for each mean difference using the CIP percentage limits.…”

Section: Methodsmentioning

confidence: 99%

“…A CV of 4.3% was used for the EPOC (CV E ) 10 and 4.0% for the manual PCV method (CV MM ). 11 Limits of agreement derived from these CVs were calculated in MS Excel using the following formulae 8,12 :…”

Section: Statistical Analysis Was Performed Using Medcalc Statisticalmentioning

confidence: 99%

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Comparison of three hematocrit measurement methods in the southern white rhinoceros (Ceratotherium simum simum)

Steyrer

Pohlin

Meyer

et al. 2022

Veterinary Clinical Pathol

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Background: Hematocrit (HCT) determination is an integral part of health and disease assessments in captive and wild white rhinoceroses. Several affordable automated hematology analyzers have been developed for in-clinic and field use and have the advantage of being able to measure a large number of additional measurands. However, the accuracy of these analyzers for rhinoceros HCT measurements has not yet been investigated. Objectives:We aimed to compare the HCT results generated by the EPOC portable analyzer system and the Abaxis VetScan HM5 with the gold standard of a manual packed cell volume (PCV) measured using the microhematocrit method.Methods: Hematocrits were measured with the EPOC and the Abaxis VetScan HM5 (bovine setting) and compared with the PCVs of 69 white rhinoceros whole blood samples. Results were compared using Bland-Altman difference plots and Passing-Bablok regression analysis. A total allowable analytical error of 10% was set as the performance goal.Results: A significant positive bias, with a mean of 7.7% for the EPOC and 17.9% for the Abaxis, was found compared with the manual PCV method. Conclusions:The allowable error goal of 10% was not exceeded with the EPOC analyzer. Although not analytically equivalent to the gold standard, the EPOC results could therefore be used as approximations in critical situations where manual measurements cannot be performed. The Abaxis exceeded this allowable error and overestimated HCTs in rhinoceroses. Therefore, method-specific reference intervals should be used.

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Section: Discussionmentioning

confidence: 99%

CIP upper and lower limits for EPOC ()(%) goodbreak= 0 goodbreak\pm 1.96 goodbreak\times \sqrt{{CV}_{E}^{2} + {CV}_{MM}^{2}}

CIP upper and lower limits for Abaxis ()(%) goodbreak= 0 goodbreak\pm 1.96 goodbreak\times \sqrt{{CV}_{A}^{2} + {CV}_{MM}^{2}}

Section: Methodsmentioning

confidence: 99%

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Comparison of three hematocrit measurement methods in the southern white rhinoceros (Ceratotherium simum simum)

Steyrer

Pohlin

Meyer

et al. 2022

Veterinary Clinical Pathol

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“…A manual PCV and total plasma protein were performed on each EDTA systemic blood sample using a standard, previously described technique 13 . Blood typing was performed by use of the relevant species‐specific QuickTest a,b blood typing standard ICC as per manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the utility and accuracy of body fluids containing red blood cells to determine canine and feline blood types

García‐Arce

Breheny

Boag

et al. 2022

J Vet Emergen Crit Care

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Objective To examine the accuracy of using body fluids macroscopically suspected to contain erythrocytes to determine the blood type in dogs and cats by use of an immunochromatographic cartridge (ICC), compared to systemic blood as the reference standard. Design Prospective study. Setting University teaching hospital. Animals Thirty client‐owned dogs and 8 cats. Interventions Dogs and cats with a sanguineous or serosanguineous body fluid (SBF) that also required a blood sample were eligible for inclusion. PCV and blood type were determined in all blood and fluid samples. For body fluids with a low PCV and discordant blood type results compared to systemic blood, sample concentration and repeat blood typing from the fluid was performed when enough sample was available. Measurement and Main Results Body fluid samples consisted of 16 pleural (11 dogs; 5 cats), 12 peritoneal (10 dogs; 2 cats), and 4 canine pericardial effusions, 3 urine samples, and 1 each of feces and epistaxis from dogs and a seroma sample from a cat. Median (range) manual PCV of blood and fluid samples was 34% (14%–66%) and 6% (0.5%–70%) for dogs and 28% (14%–48%) and 14% (0.5%–19%) for cats, respectively. Dogs were correctly classified as being DEA 1 negative, DEA 1 positive, and DEA 1 weak positive when using body fluid for blood typing 13 of 14, 4 of 9, and 5 of 7, respectively. All reference blood type to fluid blood type (FBT) discordant results had a body fluid PCV equal to or below 2%. Subsequently concentrated body fluid samples had a PCV above 8% and repeat FBT matched reference blood type (RBT). All cats were classified as type A by all RBTs and FBTs. Conclusions Body fluids containing erythrocytes may be utilized to blood type dogs if sufficiently concentrated and type A cats.

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“…The system composed of Arduino board, red light (600nm), photocell, and LCD. However, the system has limited samples to analysis [14].…”

Section: Introductionmentioning

confidence: 99%

MATLAB modeling to evaluate the ratio of packed cell volume

Alwan

Yaseen²,

Jameel³

2021

IJEECS

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<p>There are many direct and indirect methods of measuring the volumetric ratioof red blood cells relative to sample size and total blood, but the manual carcinogenic method remains the most stable and least expensive method despite the possibility of human error.The ratio percent of the packed red blood cell volume to the total volume of the blood sample is known as the packed cell volume (PCV). Our efforts were directed at finding a precise and economic method for PCV determination. There by, we designed a simple device consisting of a digital camera that is connected to a computer which uses a specific MATLAB program to process the blood images. As a result,the image can be read and the PCV can be calculated using a special code system. This new measuring system provides the capabilities and advantagesto frequently comment on measured PCV with normal or abnormal, in comparison with normal values of PCV, which are stored in the computer. Our measurement method provides accurate results compared to the ruler results to alleviate the effort of clearing the hepatitis C virus from the blood (PCV). This system enables a swift and simple sample analysis and PCV identification.</p>

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Standard operating procedure reduces interoperator variation and improves accuracy when measuring packed cell volume

Cited by 7 publications

References 5 publications

Comparison of three hematocrit measurement methods in the southern white rhinoceros (Ceratotherium simum simum)

Comparison of three hematocrit measurement methods in the southern white rhinoceros (Ceratotherium simum simum)

Evaluation of the utility and accuracy of body fluids containing red blood cells to determine canine and feline blood types

MATLAB modeling to evaluate the ratio of packed cell volume

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