Standard methods for instrumental insemination of <i>Apis mellifera</i> queens

Cobey, Susan; Tarpy, David R.; Woyke, J.

doi:10.3896/ibra.1.52.4.09

Cited by 120 publications

(122 citation statements)

References 28 publications

Supporting

Mentioning

120

Contrasting

Unclassified

Order By: Relevance

“…or instrumental insemination (see the BEEBOOK paper on instrumental insemination, Cobey et al, 2013). The aim of this kind of design is to rapidly achieve improvement while limiting loss of genetic variability (which would lead to inbreeding depression).…”

Section: Closed-population Breedingmentioning

confidence: 99%

“…In addition to the welldeveloped instrumental insemination technique (see the BEEBOOK paper on instrumental insemination (Cobey et al, 2013)) isolated mating stations can serve as an efficient technique for control of honey bee mating for commercial and scientific purposes.…”

Section: Mating Controlmentioning

confidence: 99%

“…For this aim, instrumental insemination (see the BEEBOOK paper on instrumental insemination (Cobey et al, 2013)) of one or more queens with semen from a single drone can be used (spermatozoa of a single drone are genetically identical). According to the number of individuals needed for the experiment, the scientist may decide whether to inseminate up to 3 queens with semen from a single drone.…”

Section: Single Drone Matingmentioning

confidence: 99%

See 2 more Smart Citations

Standard methods for rearing and selection ofApis melliferaqueens

Büchler

Andonov

Bienefeld

et al. 2013

Journal of Apicultural Research

194

200

View full text Add to dashboard Cite

SummaryHere we cover a wide range of methods currently in use and recommended in modern queen rearing, selection and breeding. The recommendations are meant to equally serve as standards for both scientific and practical beekeeping purposes. The basic conditions and different management techniques for queen rearing are described, including recommendations for suitable technical equipment. As the success of breeding programmes strongly depends on the selective mating of queens, a subchapter is dedicated to the management and quality control of mating stations. Recommendations for the handling and quality control of queens complete the queen rearing section. The improvement of colony traits usually depends on a comparative testing of colonies. Standardized recommendations for the organization of performance tests and the measurement of the most common selection characters are presented. Statistical methods and data preconditions for the estimation of breeding values which integrate pedigree and performance data from as many colonies as possible are described as the most efficient selection method for large populations. Alternative breeding programmes for small populations or certain scientific questions are briefly mentioned, including also an overview of the young and fast developing field of molecular selection tools. Because the subject of queen rearing and selection is too large to be covered within this paper, plenty of references are given to facilitate comprehensive studies. Métodos estándar para la cría y selección de reinas de Apis mellifera ResumenSe describe una amplia gama de métodos actualmente en uso y recomendables sobre la cría actual de reinas, su selección y cruzamiento. Las recomendaciones tienen el propósito de servir de igual forma como estándares para fines apícolas tanto científicos como prácticos. Se describen las condiciones básicas y las diferentes técnicas de manejo para la cría de reinas, incluyendo recomendaciones para el equipo técnico adecuado. Dado que el éxito de los programas de mejora depende en gran medida el apareamiento selectivo de reinas, se dedica un subcapítulo a la gestión y control de calidad de las estaciones de apareamiento. Las recomendaciones para el manejo y control de calidad de las reinas completan la sección de cría de reinas. La mejora de las características de colonias por lo general, depende de ensayos comparativos entre colonias. Se presentan recomendaciones normalizadas para la organización de pruebas de rendimiento y la medición de los caracteres de selección más comunes. Aquellos métodos estadísticos y condiciones previas de datos para la estimación de valores de cruzamiento que integren los datos genealógicos y de rendimiento de tantas colonias como sea posible, se describen como los métodos de

show abstract

Section: Closed-population Breedingmentioning

confidence: 99%

Section: Mating Controlmentioning

confidence: 99%

Section: Single Drone Matingmentioning

confidence: 99%

See 1 more Smart Citation

Standard methods for rearing and selection ofApis melliferaqueens

Büchler

Andonov

Bienefeld

et al. 2013

Journal of Apicultural Research

194

200

View full text Add to dashboard Cite

show abstract

“…Drone endophali and/or semen can be collected by squeezing out the endophallus as described in the BEEBOOK article on artificial insemination (Cobey et al, 2013). The endophallus or semen can be transported in tubes or on collection cards, on ice.…”

Section: Drone Endophallus and Semenmentioning

confidence: 99%

Standard methods for virus research inApis mellifera

Miranda

Bailey

Ball

et al. 2013

Journal of Apicultural Research

264

254

View full text Add to dashboard Cite

SummaryHoney bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual and colony-level symptoms, transmission and epidemiology. The research methods used in virology are therefore equally diverse.This article covers those methods that are very particular to virological research in bees, with numerous cross-referrals to other BEEBOOK papers on more general methods, used in virology as well as other research. At the root of these methods is the realization that viruses at their most primary level inhabit a molecular, subcellular world, which they manipulate and interact with, to produce all higher order phenomena associated with virus infection and disease. Secondly, that viruses operate in an exponential world, while the host operates in a linear world and that much of the understanding and management of viruses hinges on reconciling these fundamental mathematical differences between virus and host. The article concentrates heavily on virus propagation and methods for detection, with minor excursions into surveying, sampling management and background information on the many viruses found in bees. Métodos estándar para la investigación de virus en Apis mellifera ResumenLa investigación de los virus de la abeja de la miel es un área sumamente amplia, que abarca desde la biología molecular subcelular hasta la fisiología y el comportamiento, desde síntomas al nivel de individuo hasta al nivel de la colmena, transmisión y epidemiología. Los métodos de investigación en virología son, por tanto, diversos. Este artículo incluye aquellos métodos específicos de la investigación virológica en las abejas, con numerosas referencias cruzadas con otros artículos del BEEBOOK y otros más generales, usados tanto en virología como en otras disciplinas. La base de estos métodos es la comprensión de los virus en su nivel primario de hábitat molecular, ambiente subcelular, que manipulan y con el que interactúan, para producir otros fenómenos de orden superior asociados a la infección del virus y la enfermedad. En segundo lugar, estos virus actúan en un mundo exponencial, mientras que los hospedadores actúan en un mundo lineal y gran parte del entendimiento y manejo de los virus depende de los fundamentos matemáticos de las diferencias entre el virus y el hospedador. El artículo se centra principalmente en la propagación de virus y en los métodos para su detección, con inclusiones menores en su estudio, el manejo del muestreo y la información general sobre los numerosos virus que se encuentran en las abejas.

show abstract

“…Male semen was collected by provoking the organ to evert by slightly bending the thorax and pressing it with the fingers, following the method described by Cobey et al (2013). Each male provided approximately 1 μL of ejaculate.…”

Section: Honeybee Males and Semen Collectionmentioning

confidence: 99%

Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

et al. 2015

View full text Add to dashboard Cite

-Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species and antioxidant defences. We measured total antioxidant capacity (TAC) in honeybee haemolymph and seminal plasma and analysed TAC of haemolymph in relation to age and exposure to pesticide. TAC of haemolymph increased with age of bees (1.18 vs 1.97 mM of (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) for 1-and 30-day-old bees, respectively, P ≤0.05). Exposure to imidacloprid (IMD) affected TAC of haemolymph of 1-day-old but not 30-day-old honeybees. TAC in haemolymph of 1-day-old bees was lower in treatments with the addition of 5 and 200 ppb IMD (1.57-1.46 mM of Trolox in treated bees compared with 2.37 mM of Trolox in controls; P ≤0.05). In conclusion, antioxidant protection of honeybees seems to be related to age and may be disturbed by exposure to IMD. Older bees with higher antioxidant protection seem to be less susceptible to IMD toxicity. The toxic effect of pesticide seems to be particularly dangerous in early life stages of honeybees.Apis mellifera / total antioxidant capacity / haemolymph / ageing / pesticide

show abstract

Standard methods for instrumental insemination of Apis mellifera queens

Cited by 120 publications

References 28 publications

Standard methods for rearing and selection ofApis melliferaqueens

Standard methods for rearing and selection ofApis melliferaqueens

Standard methods for virus research inApis mellifera

Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Contact Info

Product

Resources

About