Standard Method Performance Requirements (SMPRs®) 2014.003: Revised: Determinional of GOS in Infant Formula and Adult/Pediatric Nutritional Formula

Determination of β-Galactooligosaccharides (GOS) in Infant Formula and Adult Nutritionals: Single-Laboratory Validation, First Action 2021.01

“…The LOD and LQQ are difficult to estimate since they depend on the profile of the GOS ingredient being used; therefore spike–recovery experiments were performed at 0.2 g/100 g, the required LOQ assigned in the SMPR ( 25 ). Spike–recovery results and the method precision at the addition level of 0.2 g/100 g were in line with the requirements of the SMPR, and thus it appears the method meets the LOQ requirements of the SMPR.…”

“…The SMPR ( 25 ) requires that the RSD r should be less than or equal to 6%. The achieved RSD r was less than 6%, for all matrices except one (No.…”

“…Table 1 summarizes the main requirements described in Standard Method Performance Requirements (SMPR ® ) 2014.003 ( 25 ) for the Determination of GOS in Infant Formula and Adult Nutritionals. The SLV was designed to test the method against those requirements.…”

“… a From SMPR 2014.003 ( 25 ). b Concentrations apply to the product as consumed (i.e., on reconstituted powders or concentrates or “as is” on ready to feed (RTF) products).…”

“…LOD and LOQBecause the method requires the analysis of a complete profile of oligosaccharides, the detection and quantification limits depend on the GOS profile as well as the concentration of GOS in the product, making it extremely difficult to assess (except on an individual oligosaccharide basis). To demonstrate method applicability at the LOQ defined in the SMPR ( 25 ), spike–recovery experiments have been performed at the desired LOQ (0.2 g/100 g).…”