Abstract:Grafting by vegetables is a practice with many benefits, but also with some unknown influences on the chemical composition of the fruits. Our goal was to assess the effects of grafting and storage on the extracted juice of four orange-fleshed Cantaloupe type (Celestial, Donatello, Centro, Jannet) melons and two green-fleshed Galia types (Aikido, London), using sensory profile analysis and analytical instruments: An electronic tongue (E-tongue) and near-infrared spectroscopy (NIRS). Both instruments are known f… Show more
“…As well as distinguishing elderberry genotypes based on fruit quality [ 10 ]. NIR spectroscopy is a very powerful tool for analysis of fruits and vegetables and related food products [ 11 , 12 ]. Our study is the first that describes a NIR spectroscopy technique for the determination of TAC in elderberry.…”
Elderberry (Sambucus nigra L., fructus) is a very potent herbal drug, deriving from traditional European medicine (TEM). Ripe elderberries are rich in anthocyanins, flavonols, flavonol esters, flavonol glycosides, lectins, essential oils, unsaturated fatty acids and vitamins. Nevertheless, unripe elderflower fruits contain a certain amount of sambunigrin, a toxic cyanogenic glycoside, whose concentration decreases in the ripening process. Therefore, quality assurance must be carried out. The standard method described in literature is the photometric determination (pH-differential method) of the total anthocyanin content (TAC) that is the highest when the berries are ripe. The drawback of the pH-differential method is the extensive sample preparation and the low accuracy of the method. Therefore, the goal of this publication was to develop a fast non invasive near-infrared (NIR) method for the determination of TAC in whole berries. TAC of elderberries was measured using pH-differentiation method where TAC values of 632.87 mg/kg to 4342.01 mg/kg were measured. Additionally, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside and cyanidin-3-O-sambubioside-5-O-glucoside which represent more than 98% of TAC in elderberry were quantified using ultra high performance liquid chromatography-multiple wavelength detection—ultra high resolution-quadrupole-time of flight-mass spectrometry (UHPLC-MWD-UHR-Q-TOF-MS) and their sum parameter was determined, ranging between 499.43 mg/kg and 8199.07 mg/kg. Using those two methods as reference, whole elderberries were investigated by NIR spectroscopy with the Büchi NIRFlex N-500 benchtop spectrometer. According to the constructed partial least squares regression (PLSR) models the performance was as follows: a relative standard deviation (RSDPLSR) of 13.5% and root mean square error of calibration (RMSECV/RMSEC) of 1.31 for pH-differentiation reference and a RSDPLSR of 12.9% and RMSECV/RMSEC of 1.28 for the HPLC reference method. In this study, we confirm that it is possible to perform a NIR screening for TAC in whole elderberries. Using quantum chemical calculations, we obtained detailed NIR band assignments of the analyzed compounds and interpreted the wavenumber regions established in PLSR models as meaningful for anthocyanin content. The NIR measurement turned out to be a fast and cost-efficient alternative for the determination of TAC compared to pH-differential method and UHPLC-MWD-UHR-Q-TOF-MS. Due to the benefit of no sample preparation and extraction the technology can be considered as sustainable green technology. With the above mentioned inversely proportional ratio of TAC to total amount of toxic cyanogenic glycosides, NIR proves to be a reliable screening method for the ideal harvest time with maximal content of TAC and lowest content of cyanogenic glycosides in elderberry.
“…As well as distinguishing elderberry genotypes based on fruit quality [ 10 ]. NIR spectroscopy is a very powerful tool for analysis of fruits and vegetables and related food products [ 11 , 12 ]. Our study is the first that describes a NIR spectroscopy technique for the determination of TAC in elderberry.…”
Elderberry (Sambucus nigra L., fructus) is a very potent herbal drug, deriving from traditional European medicine (TEM). Ripe elderberries are rich in anthocyanins, flavonols, flavonol esters, flavonol glycosides, lectins, essential oils, unsaturated fatty acids and vitamins. Nevertheless, unripe elderflower fruits contain a certain amount of sambunigrin, a toxic cyanogenic glycoside, whose concentration decreases in the ripening process. Therefore, quality assurance must be carried out. The standard method described in literature is the photometric determination (pH-differential method) of the total anthocyanin content (TAC) that is the highest when the berries are ripe. The drawback of the pH-differential method is the extensive sample preparation and the low accuracy of the method. Therefore, the goal of this publication was to develop a fast non invasive near-infrared (NIR) method for the determination of TAC in whole berries. TAC of elderberries was measured using pH-differentiation method where TAC values of 632.87 mg/kg to 4342.01 mg/kg were measured. Additionally, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside and cyanidin-3-O-sambubioside-5-O-glucoside which represent more than 98% of TAC in elderberry were quantified using ultra high performance liquid chromatography-multiple wavelength detection—ultra high resolution-quadrupole-time of flight-mass spectrometry (UHPLC-MWD-UHR-Q-TOF-MS) and their sum parameter was determined, ranging between 499.43 mg/kg and 8199.07 mg/kg. Using those two methods as reference, whole elderberries were investigated by NIR spectroscopy with the Büchi NIRFlex N-500 benchtop spectrometer. According to the constructed partial least squares regression (PLSR) models the performance was as follows: a relative standard deviation (RSDPLSR) of 13.5% and root mean square error of calibration (RMSECV/RMSEC) of 1.31 for pH-differentiation reference and a RSDPLSR of 12.9% and RMSECV/RMSEC of 1.28 for the HPLC reference method. In this study, we confirm that it is possible to perform a NIR screening for TAC in whole elderberries. Using quantum chemical calculations, we obtained detailed NIR band assignments of the analyzed compounds and interpreted the wavenumber regions established in PLSR models as meaningful for anthocyanin content. The NIR measurement turned out to be a fast and cost-efficient alternative for the determination of TAC compared to pH-differential method and UHPLC-MWD-UHR-Q-TOF-MS. Due to the benefit of no sample preparation and extraction the technology can be considered as sustainable green technology. With the above mentioned inversely proportional ratio of TAC to total amount of toxic cyanogenic glycosides, NIR proves to be a reliable screening method for the ideal harvest time with maximal content of TAC and lowest content of cyanogenic glycosides in elderberry.
“…Grieneisen et al [15] conducted a review of 202 different rootstocks and 1023 experimental treatments related to tomato grafting, and they concluded that fruit quality data based on sensory tests were rare among the published studies. Sensory evaluation of grafted plants of the members of the Cucurbitaceae family, i.e., melon [16], watermelon [17,18], and cucumber [19], and those of the Solanaceae family [20,21] have been conducted, but there is relatively few data for eggplant.…”
This study aimed to investigate the effect of different rootstocks on the yield and quality of eggplant cv. ‘Madonna’ in soilless pot culture in an unheated polyethylene greenhouse. The eggplant was grafted onto several rootstocks, including tomato rootstocks Optifort (O) and Emperador (E), and four Solanum rootstocks; Solanum grandiflorum × Solanum melongena (SH), Solanum torvum (ST), Solanum melongena × Solanum integrifolium (SI), and Solanum integrifolium (A) compared with self-grafted (SG) and self-rooted (SR) as control. The results showed that the total marketable yield significantly increased by grafting onto ST (3.94 kg/plant), SH (3.36 kg/plant), and A (3.34 kg/plant) relative to SR (1.65 kg/plant). The chromatics characters of skin and pulp are slightly influenced by rootstocks. Our findings confirmed that grafting eggplant decreased firmness (except SH) of the flesh. Fruit harvested from the Optifort/Madonna combination had the rounded shape, lowest firmness, and Brix value, while the lowest oxidation potential was observed in this combination. The highest seed number was observed in SH/Madonna and SI/Madonna combinations. During the sensory evaluation, the lightest fruit flesh was found in SR, ST, and O, and the sweetest taste was observed in fruits harvested from ST rootstock.
“…This substantiates the importance of NIR spectroscopy for rapid monitoring of additives and preservatives in fruit juices when either transmittance, reflectance, or diffuse-reflectance mode is used. In combination with standard analytical techniques, both a benchtop and handheld SMC NIR in reflectance mode could discriminate different varieties of green and orange fleshed melons with 100% classification accuracy in LDA and predict multiple sensory parameters in PLS regression with high precision (R 2 up to 0.96) and low errors (RMSECV as low as 0.24 g/mL) after cross-validation [ 80 ]. Transmittance mode benchtop FT-NIR provided high R 2 in cross-validation (up to 0.94) and low errors (RMSECV as low as 5.07 mg nitrogen/L) when it was used to measure total yeast assimilable nitrogen, free amino nitrogen, and ammonia in 900 grape juice samples from 28 cultivars over three seasons [ 81 ].…”
Section: Near Infrared (Nir) Spectroscopy: Historical Background Amentioning
confidence: 99%
“…The same potentiometric E-tongue measurements combined with LDA showed 100% separation between the flesh of different colors. Good correlations were found between some standard analytical parameters and E-tongue sensor signals with PLS regression models [ 80 ].…”
Section: Electronic Tongue: Historical Background and Food Qualitymentioning
Amid today’s stringent regulations and rising consumer awareness, failing to meet quality standards often results in health and financial compromises. In the lookout for solutions, the food industry has seen a surge in high-performing systems all along the production chain. By virtue of their wide-range designs, speed, and real-time data processing, the electronic tongue (E-tongue), electronic nose (E-nose), and near infrared (NIR) spectroscopy have been at the forefront of quality control technologies. The instruments have been used to fingerprint food properties and to control food production from farm-to-fork. Coupled with advanced chemometric tools, these high-throughput yet cost-effective tools have shifted the focus away from lengthy and laborious conventional methods. This special issue paper focuses on the historical overview of the instruments and their role in food quality measurements based on defined food matrices from the Codex General Standards. The instruments have been used to detect, classify, and predict adulteration of dairy products, sweeteners, beverages, fruits and vegetables, meat, and fish products. Multiple physico-chemical and sensory parameters of these foods have also been predicted with the instruments in combination with chemometrics. Their inherent potential for speedy, affordable, and reliable measurements makes them a perfect choice for food control. The high sensitivity of the instruments can sometimes be generally challenging due to the influence of environmental conditions, but mathematical correction techniques exist to combat these challenges.
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