Standalone cell culture microfluidic device-based microphysiological system for automated cell observation and application in nephrotoxicity tests

Kimura, Hiroshi; Nakamura, Hiroko; Goto, Tomomi; Uchida, Wakana; Uozumi, Takayuki; Nishizawa, Daniel; Shinha, Kenta; Sakagami, Junko; Doi, Kotaro

doi:10.1039/d3lc00934c

Cited by 1 publication

(1 citation statement)

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“…To perform the high-throughput screening of combinatorial drugs, we propose a microfluidic chip which can generate mixtures of different drug doses with minimum effort (Figure 1). The primary material of these microfluidic chips, PDMS, is highly biocompatible, allowing for precise control of the microenvironment in long-term cell cultures and facilitating evaluations of drug screening and delivery [35][36][37][38][39][40][41][42]. The microfluidic chip is 6 cm in length, 5 cm in width, and 0.5 cm in height.…”

Section: Design and Characterization Of Microfluidic Chipsmentioning

confidence: 99%

A Multi-Drug Concentration Gradient Mixing Chip: A Novel Platform for High-Throughput Drug Combination Screening

Fu,

Feng,

Sun

et al. 2024

Biosensors

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Combinatorial drug therapy has emerged as a critically important strategy in medical research and patient treatment and involves the use of multiple drugs in concert to achieve a synergistic effect. This approach can enhance therapeutic efficacy while simultaneously mitigating adverse side effects. However, the process of identifying optimal drug combinations, including their compositions and dosages, is often a complex, costly, and time-intensive endeavor. To surmount these hurdles, we propose a novel microfluidic device capable of simultaneously generating multiple drug concentration gradients across an interlinked array of culture chambers. This innovative setup allows for the real-time monitoring of live cell responses. With minimal effort, researchers can now explore the concentration-dependent effects of single-agent and combination drug therapies. Taking neural stem cells (NSCs) as a case study, we examined the impacts of various growth factors—epithelial growth factor (EGF), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF)—on the differentiation of NSCs. Our findings indicate that an overdose of any single growth factor leads to an upsurge in the proportion of differentiated NSCs. Interestingly, the regulatory effects of these growth factors can be modulated by the introduction of additional growth factors, whether singly or in combination. Notably, a reduced concentration of these additional factors resulted in a decreased number of differentiated NSCs. Our results affirm that the successful application of this microfluidic device for the generation of multi-drug concentration gradients has substantial potential to revolutionize drug combination screening. This advancement promises to streamline the process and accelerate the discovery of effective therapeutic drug combinations.

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Section: Design and Characterization Of Microfluidic Chipsmentioning

confidence: 99%

A Multi-Drug Concentration Gradient Mixing Chip: A Novel Platform for High-Throughput Drug Combination Screening

Fu,

Feng,

Sun

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

Standalone cell culture microfluidic device-based microphysiological system for automated cell observation and application in nephrotoxicity tests

Abstract: The SCCMD for MPS experiments, which integrates microfluidic chips and their peripherals, complies with the ANSI/SLAS standards and has been seamlessly integrated into an existing automatic cell imaging system for online cell observation.

Cited by 1 publication

References 41 publications

A Multi-Drug Concentration Gradient Mixing Chip: A Novel Platform for High-Throughput Drug Combination Screening

A Multi-Drug Concentration Gradient Mixing Chip: A Novel Platform for High-Throughput Drug Combination Screening

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