Staining techniques for detection of acid fast bacilli: what hope does fluorescein-diacetate (FDA) vitality staining technique represent for the monitoring of tuberculosis treatment in resource limited settings

Zingué, Dezemon; Muvunyi, Claude Mambo; Jacob, Otu

doi:10.7243/2055-0901-1-1

Cited by 12 publications

(7 citation statements)

References 11 publications

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“…1 Auramine-O stained sputum smear examined by LED microscopy was developed mainly to give resource-limited countries access to the benefits of fluorescence microscopy. 3 They have better sensitivity and efficiency when compared to conventional fluorescent microscopy. 3 Our study analyzes the clinical utility of the GeneXpert assay with LED-FM for the diagnosis of pulmonary tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

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A study to analyze the differences between GeneXpert MTB/RIF assay and LED fluorescent microscopy for the diagnosis of pulmonary tuberculosis in a tertiary care centre

Kuruvilla¹,

Majumder

2020

IJMR

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Mycobacterium tuberculosis cultures although being the gold standard for diagnosing tuberculosis has its limitations as it is time consuming. Thus newer methods using light-emitting diode fluorescent microscopy (LED-FM) and molecular methods like the GeneXpert MTB/RIF assay are being adopted as a mainstay for diagnosing tuberculosis around the globe. Materials and Methods: This observational descriptive study was carried out for a period of 21 months. Sputum samples from 110 adult subjects with a clinical suspicion of pulmonary tuberculosis (PTB) were stained for LED-FM microscopy and also inoculated into the GeneXpert assay. The positive smears were graded as per the Revised National Tuberculosis Control Programme (RNTCP) guidelines and positive GeneXpert assay results along with Rifampicin resistance were noted and analysed. Results: Out of the 110 samples, (77.2%) were males and (22.7%) females. Of the 110 samples, 75 (68.1%) were positive for Mycobacterium tuberculosis by the GeneXpert assay. In contrast with LED-FM, all 25 smear positive samples were also GeneXpert positive. Additionally, GeneXpert detected 10 smear negative cases. 10 (9%) positive GeneXpert isolates showed resistance to Rifampicin. The overall sensitivity of GeneXpert was greater but its specificity was comparable with LED-FM. Conclusion:The Xpert and LED-FM tests were comparable in specificity, but the GeneXpert assay out did the LED-FM as it was a more sensitive and rapid test for the diagnosis of tuberculosis and additionally detection of Rifampicin resistance. Our study fully agrees that the GeneXpert assay is a step ahead in the early diagnosis of tuberculosis even for smear negative cases of PTB. This assay is also a relatively simple and routine test that staff can perform with minimal training.

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Section: Introductionmentioning

confidence: 99%

“…3 They have better sensitivity and efficiency when compared to conventional fluorescent microscopy. 3 Our study analyzes the clinical utility of the GeneXpert assay with LED-FM for the diagnosis of pulmonary tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

A study to analyze the differences between GeneXpert MTB/RIF assay and LED fluorescent microscopy for the diagnosis of pulmonary tuberculosis in a tertiary care centre

Kuruvilla¹,

Majumder

2020

IJMR

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“…will not. [5,6] In the cold staining technique, Kinyoun used higher concentrations of basic fuchsin and phenol. [7] Tan Thia Hok devised a method by combining the staining techniques of Kinyoun and Gabbet, and found it quicker than ZN method.…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Ziehl-Neelsen and Kinyoun Staining in the Diagnosis of Clinically Suspected Cases of Pulmonary Tuberculosis

Mishra¹,

Mishra²

2019

APJHS

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Background: Bacteriological examination of sputum is the cornerstone in diagnosis of pulmonary tuberculosis in developing world, which is usually done using a Ziehl-Nelseen (ZN) method. However, due to limited laboratory facilities that can satisfy the procedure, applicability of this procedure appears to be adversely affected in field conditions and at peripheral health institutions. Hence, it has become necessary to look for a procedure which can be used as alternative in such conditions. Material and Methods: This was a cross sectional study conducted in the

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“…SDS may also inactivate M. tuberculosis , thus, potentially protecting laboratory technicians from infectious contagion during the preparation and examination of SSM slides. It has to be noted that regular TB SSM with Ziehl–Neelsen (ZN) and Auramine/Rhodamine (A/R) staining do not discriminate between viable and inactivated bacteria (Dezemon, Muvunyi & Jacob, 2014). We hypothesized that creating a more uniform sample with better homogenization could improve the clarity of SSM slides, the detection of AFB and the level of detection.…”

Section: Introductionmentioning

confidence: 99%

Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy

Sarro

Kodio

Kumar

et al. 2019

GHI

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Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether the addition of sodium dodecyl sulfate (SDS), a detergent that thins sputum, at 4% and 10%, improves the detection of acid-fast bacilli (AFB), the clarity of slides, and the biosafety of the technique. Thirty participants with presumptive TB were enrolled. Three independent, blinded technicians examined the slides. Regular sputum concentrated AFB smear and sputum culture were used as standard control methods. Sputum culture was also performed before and after 10% SDS addition for safety analysis. We found that neither SSM with SDS 4% nor SSM with SDS 10% improved the test’s performance. However, slides with 4% and 10% SDS, compared with slides prepared without SDS, had significantly better clarity scores. The 10% SDS-prepared sputum samples were all culture negative. While adding SDS detergent does not improve the performance of SSM slides, it does improve the clarity and biosafety. Where experienced technicians are scarce, especially in low resource settings, use of SDS may enhance the ease of slide reading in sputum smear microscopy.

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Staining techniques for detection of acid fast bacilli: what hope does fluorescein-diacetate (FDA) vitality staining technique represent for the monitoring of tuberculosis treatment in resource limited settings

Cited by 12 publications

References 11 publications

A study to analyze the differences between GeneXpert MTB/RIF assay and LED fluorescent microscopy for the diagnosis of pulmonary tuberculosis in a tertiary care centre

A study to analyze the differences between GeneXpert MTB/RIF assay and LED fluorescent microscopy for the diagnosis of pulmonary tuberculosis in a tertiary care centre

Comparative Evaluation of Ziehl-Neelsen and Kinyoun Staining in the Diagnosis of Clinically Suspected Cases of Pulmonary Tuberculosis

Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy

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