Staining: practical and theoretical

Gurr, Edward

doi:10.5962/bhl.title.4476

Cited by 108 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cross sections (5 μm thick) of the fixed tissue were cut using microtome (Leica PM 2125, Germany). These sections were then stained with hematoxylin and eosin method (Gurr [ 12 ]) and visualized under light microscope (Magnus, India) to study the microscopic architecture of the testis.…”

Section: Methodsmentioning

confidence: 99%

Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration

et al. 2014

View full text Add to dashboard Cite

BackgroundNanoparticles (Ag NPs) have recently received much attention for their possible applications in biotechnology and biomedical. However, little is known about the toxicity in reproductive organs of animal model following exposure to nanoparticles.ObjectiveThis study therefore, tried to examine the effects of nanoparticles with a diameter range of 5-20 nm on the histology of the testis of wistar rats and correlate it with Transmission Electron Microscopy results.Materials and methodsSixteen wistar rats were randomly divided into two groups of 8 rats each. Each group received the following via gavage technique for 90 days: Control Group (Group-1)-tap water; Experimental group (Group 2) - nanoparticles (20ug/kg/day). After ninety days (chronic study), rats were sacrificed and testis tissues was processed for histology and transmission electron microscopic study.ResultsThere was significant difference between the observations of group-1 and group 2. The changes observed in the testis were disarray of the spermatogenic cells and disorientation of the testis. These changes were observed to have been disappearing from normal histological features. Detailed structural damages were observed with TEM analysis, such as depletion of germ cells, germinal cells necrosis, especially in spermatogonia and Leydig cells had an abnormal fibroblast-like appearance, abnormal space between neighboring sertoli cells, mitochondria, lost cristae and vacuolated (none energized) with those animals exposed to nanoparticles.ConclusionIt seems that nanoparticles have acute and significant effects on spermatogenesis and number of spermatogenic cells. More experimental investigations are necessary to elucidate better conclusion regarding the safety of nanoparticles on male reproduction system.

show abstract

Section: Methodsmentioning

confidence: 99%

Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration

et al. 2014

View full text Add to dashboard Cite

show abstract

“…23 Moreover, in 1962, Gurr listed several stains used in modern electron microscopy, comprising gold chloride for nerve fibers (planarians), neuroglial fibers, astrocytes, nerve sheaths, and cells. 24 In 1984, El-Yazigi et al evaluated gold and other elements in the cerebrospinal fluid (CSF) of patients with brain tumors or cerebral neoplasms. 25 Interestingly, although differences between the concentration of gold in controls and other tumor types were achieved, there was no consistent relationship between gold levels in controls and patients with pineal blastoma.…”

Section: Bella B Manshianmentioning

confidence: 99%

Gold nanostructures: synthesis, properties, and neurological applications

Zare¹,

Yaraki

Speranza

et al. 2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…Methylene blue (MB, 3,7-bis(dimethylamino)phenothiazin-5-ium chloride) is a basic, water-soluble, nontoxic, blue, cationic thiazine dye. , It is used as an oxidation−reduction indicator in chemistry and biology, as a sensitizer in photo-oxygenation, and as an electron exchanger with photogalvanic effect. It has several medicinal applications such a treating urinary tract infections, distinguishing between cancerous and normal tissue, etc. As a secondary use, it is effective against various external protozoans, including ichthyophthirius, chilodonella, ichthyobodo (costia), and epistylis.…”

Section: Introductionmentioning

confidence: 99%

Solvent Effect on the Electronic Spectra of Azine Dyes under Alkaline Condition

et al. 2007

View full text Add to dashboard Cite

Thiazine dye, methylene blue (MB), oxazine dye, nile blue (NB), and phenazine-based dye, neutral red (NR), bear a similar basic dye skeleton with a distinctively different central heteroatom. All of them are extracted into nonpolar organic solvent from alkaline solution. The role of the heteroatom on the respective dye skeletons and redox potentials of the dyes has been examined to signature the stability of the species in organic solvent and the results have been substantiated through geometry optimization and wave function analysis at the density functional theory level. The effect of solvent polarity on the electronic absorption spectra of the three nonionic benzenoid species has been investigated with an intention to investigate the solvatochromic behavior of these compounds.

show abstract

Staining: practical and theoretical

Cited by 108 publications

References 0 publications

Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration

Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration

Gold nanostructures: synthesis, properties, and neurological applications

Solvent Effect on the Electronic Spectra of Azine Dyes under Alkaline Condition

Contact Info

Product

Resources

About