Staining of the Stem Tissue of Plants by Triphenyltetrazolium Chloride

Waugh, Thomas D.

doi:10.1126/science.107.2776.275

Cited by 28 publications

(3 citation statements)

References 7 publications

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“…2,3,5-Triphenyl-2H-tetrazolium chloride (TTC) is an interesting molecule used as indicator in various biological applications, such as counting bacterial colonies, indicating viability of seeds, differential staining of tissues, or bacteriostatic action. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Its use for these purposes is based on its ability to reduce to the corresponding red formazan. This redox-based staining property serves to evaluate reductive enzyme activity, to oxidize sugars and steroids, and to photometrically determine metal activities.…”

Section: Introductionmentioning

confidence: 99%

Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

et al. 2007

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Aromatic-aromatic interactions are found between the cationic molecule 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) and the molecule poly(sodium 4-styrenesulfonate) (PSS) which makes the overall interaction of TTC with PSS more intense than the interaction with other polyanions containing sulfonate groups and produces a decrease on the redox ability of TTC. Diafiltration was used to compare the binding of TTC to PSS, poly(sodium vinylsulfonate) (PVS), and the more hydrophobic poly(sodium 2-(N-acrylamido)-2-methyl-propanesulfonate) (PAMPS). The UV-vis spectrum of TTC is changed in the presence of the aromatic polyanion. The 1H NMR signals of TTC are broadened and shifted in the presence of PSS, suggesting the occurrence of pi-pi interactions. Moreover, nuclear Overhauser effects (NOE) between the TTC and PSS protons are found. Possible structures for the complex are proposed.

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Section: Introductionmentioning

confidence: 99%

Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

et al. 2007

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“…Various studies reported tetrazolium dyes use on plant seeds (Cottrell 1947, Porter et al 1947, Bennett and Loomis 1949, Lakon 1949, plant tissues (Gall 1948, Dufrenony and Pratt 1948, Waugh 1948, Roberts 1950, human and animal tissues (Straus et al 1948, Black and Kleiner 1949, Parker 1953, mi-tochndrial localization of oxidative enzymes (Pearse 1957), and microorganisms (Fred and Knight 1949, Gunz 1949, Currier and Day 1954. Since then, the use of tetrazolium salts in different research fields has been studied intensively due to its wide potential applications and research opportunities.…”

Section: Introductionmentioning

confidence: 99%

A simple method for dehydrogenase activity visualization of intact plant roots grown in soilless culture using tetrazolium violet

2010

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____________________________________________________________________________________________________________________________________________________________________________Kurzbaum E, Kirzhner F, Armon R 2010 A simple method for dehydrogenase activity visualization of intact plant roots grown in soilless culture using tetrazolium violet. Plant Root 4: 12-16. Abstract:A simple method for the evaluation of respiration activity of root cells of intact plants grown hydroponically and/or in agar medium was developed. The novelty of the present method is based on visual detection of dehydrogenase activity of plant roots by use of tetrazolium violet dye without destructive steps, allowing follow up of living and photosynthetically active growing plants and the impact of inhibitors such as sodium azide and cycloheximide. The results of this approach demonstrated that root tip cells comprise the highest dehydrogenase activity compared to other root parts. The non-expensive assay is easy to perform and allows to experiment a large variety of chemical compounds with potential inhibitory characteristics for plants.

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“…In a previous paper discussion was focused on the interference of penicillin with H transfer, although it was pointed out that phosphorylation and dephosphorylation resulting from the transfer of P04 are fundamentally as important as hydrogenation . This paper describes the concomitant use of triphenyltetrazolium chloride (TPT), which deposits as a red formazan at sites of high reducing activity (Lakon, 1942;Cottrell, 1947;Mattson et al, 1947;Porter et al, 1947;Dufrenoy and Pratt, 1948c;Straus et al, 1948;Waugh, 1948), and of trypan blue, which was used successfully in cytochemistry by McWhorter (1941). Under proper conditions of pH, trypan blue is relatively insensitive to reduction but specifically stains ribonucleic inclusions.…”

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confidence: 99%