Staining of the Inclusion Bodies of Trachoma and Inclusion Conjunctivitis

Genital Infection in Association with TRIC Virus Infection of the Eye--II: II. CYTOLOGY. PRELIMINARY REPORT

“…Macchiavello's and Castafieda's stains, whilst satisfactory for demonstrating inclusion bodies, do not reveal the cellular pattern. Iodine staining (Rice, 1936;Gilkes, Smith, and Sowa, 1958), which is invaluable for the rapid demonstration of inclusions in conjunctival scrapings (Fig. 2a), cannot be used on genital material, because many cells, in the urethral and cervical epithelium contain glycogen and therefore stain so intensely with iodine (Jones, Collier, and Smith, 1959) that the recognition of inclusions is impossible (Fig.…”

Possible Virus Aetiology of Non-Specific Urethritis

“…oil-immersion objective. As only one dried smear was available for study from Cases 9 to 20, this was stained first with iodine and then, when the oil had been removed and the iodine washed out, it was stained with Giemsa (Gilkes, Smith, and Sowa, 1958 All the cell cultures contained coverslips and I -5 ml. tissue culture medium, and were incubated at 320 to 36°C., the tubes being inclined at an angle of 5°from the horizontal.…”

“…oil-immersion objective. As only one dried smear was available for study from Cases 9 to 20, this was stained first with iodine and then, when the oil had been removed and the iodine washed out, it was stained with Giemsa (Gilkes, Smith, and Sowa, 1958).…”

A comparison of the iodine and fluorescent antibody methods for staining trachoma inclusions in the conjunctiva

“…After delineating the smear with a wax pencil, the entire area was systematically scanned with a x 10 objective (final magnification x 120) and the total number of inclusions was counted. In the 'dry' method originally described by Gilkes et al (1958) the stained smears were covered with a thin layer of immersion oil. The use of oil to improve contrast depends on the optical properties of the microscope used; it was not necessary in this investigation.…”