Staining of Some Specific Regions of Human Chromosomes, particularly the Secondary Constriction of No. 9

Bobrow, Martin; Madan, K.; Pearson, P. L.

doi:10.1038/newbio238122a0

Cited by 235 publications

(63 citation statements)

References 14 publications

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“…An average of 82 metaphase spreads (range 45-167) were analyzed for each cell line. The methods for the 22 different isozyme markers for 17 different human chromosomes were given in our earlier reports (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). The cells used for chromosomal and isozymal analysis were from the pool of cells from which the DNA was isolated for use in the DNA-cDNA hybridization studies (2).…”

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confidence: 99%

“…Hybrid cell WL II 24a 5C was derived by fusion of the thymidine kinase-deficient L cell (LM TI-) with the human fibroblast WI-38 and isolated in hypoxanthine/aminopterin/thymidine (7). Giemsa 11 stain was used to specifically identify mouse and human chromosomes, as outlined previously (2,8,9). Single metaphase spreads were stained sequentially by a combination of Giemsa-trypsin followed by Hoechst 33258 staining to identify specific human chromosomes (2,10,11).…”

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confidence: 99%

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Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybrids

Deisseroth¹,

Nienhuis²,

Lawrence³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

166

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Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybrids

Deisseroth¹,

Nienhuis²,

Lawrence³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

166

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“…A 0 is a n intercalating fluorochrome and is being used to stain differentially double-stranded vs. denatured DNA, yielding green and red luminescence, respectively (14). After partial denaturation of DNA in situ in chromosomes by heat and staining with AO, the red-green banding is apparent, which has been interpreted as indicating a different sensitivity of DNA in the respective bands to denaturation (1,9,16). Quinacrine was studied because it is a n intercalating agent that induces classical (Q) chromosome banding (4,5) and so far no studies were done on its ability to condense DNA.…”

Section: Discussionmentioning

confidence: 99%

Condensation of DNA in situ in metaphase chromosomes induced by intercalating ligands and its relationship to chromosome banding

Darzynkiewicz

Kapuściński

1988

Cytometry

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Interactions of certain intercalating cationic ligands with nucleic acids result in the formation of products that undergo condensation and agglomeration; this transition in solution can be monitored by light-scatter measurements. In the present study, using such intercalators as the antitumor drug mitoxantrone or fluorochromes acridine orange and quinacrine, we induced condensation of DNA in situ in Chinese hamster chromosomes. The in situ products scattered light and could be detected by darkfield-or phasecontrast microscopy. In the darkfield the complexes had a characteristic granular appearance and often generated a banding pattern on the chromosomes. In contrast, condensation of DNA in situ by the nonintercalating polyvalent cations (co"+, spermine*'), while enhancing the chromosome's image contrast, did not produce the granular products or the banding. The condensation of free DNA, single or double stranded, natural or synthetic, the lab ter of various base composition and configuration, was also measured in solution. The condensation in solution and in situ was observed at similar concentrations of the respective ligands. The intercalating dye ethidium bromide, which did not condense DNA in solutions of moderate and high ionic strength, also did not generate the granular products or banding on chromosomes. The data also show that both base composition and configuration are important factors in determining the sensitivity of DNA to condensation by particular intercalating ligands.The studies suggest that the phenomenon of DNA condensation by intercalating dyes, which shows a high degree of specificity with respect to primary and secondary structures of DNA, may be associated with mechanisms of chromosome banding induced by the intercalating thiazine dyes in Giemsa staining or by quinacrine. Observation of chromosome banding based on light-scatter detection in darkfield microscopy allows the study of interactions between DNA and the ligands that neither fluoresce nor generate colored products. This principle of chromosome "counterstaining" can be explored by flow cytometry.

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“…Occurrence of blocks of constitutive heterochromatin in whole arms, is not un common among mammalian species (Bobrow et al 1972, Pathak et al 1973, Markvong et al 1975, Arrighi et al 1976, Mascarello and Hsu 1976, Robbins and Baker 1980, Patton and Sherwood 1982). Yet it is relatively rare in chiropterans (Pathak and Wurstre-Hill 1977).…”

Section: Discussionmentioning

confidence: 99%

Karyotypic architecture of the false vampire bat Megaderma lyra.

Naidu

Gururaj

1985

CYTOLOGIA

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Staining of Some Specific Regions of Human Chromosomes, particularly the Secondary Constriction of No. 9

Cited by 235 publications

References 14 publications

Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybrids

Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybrids

Condensation of DNA in situ in metaphase chromosomes induced by intercalating ligands and its relationship to chromosome banding

Karyotypic architecture of the false vampire bat Megaderma lyra.

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