Staining of Semithin Tissue Sections Embedded in HPMA, Quetol 523 and MMA

Abstract

Summary: Various tissues fixed in a mixture of formaldehyde and glutaraldehyde, and embedded in an improved 2-hydroxypropyl methacrylate mixture were employed for studying the fine structures of cells and tissues by light microscopy. The embedding mixture contained Quetol 523 and methyl methacrylate as a plasticizer without a cross-linker. The catalyst was QCU-1. The mixture had a low viscosity, was easy to handle and penetrated readily and completely into the specimen, been employed with such sections and those reported here are hematoxylin-eosin, Azan and PAS. There was excellent preservation of alkaline phosphatase activity. A method of poststaining immunoperoxidase labeling was also applied to the mouse pancreas and examples of staining with insulin are included.

Effects of immersion in 4-methacryloyloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane resin-activated liquid on microtensile bond strength of root canal dentin

“… 27 Resin impregnation techniques for biological tissues have been applied in studies on tissue sections; various resins and impregnation techniques have been studied depending on the condition of the observation target. 28 , 29 , 30 , 31 In this study the intertubular dentin showed a more reticulated structure after immersion in the Super-Bond-activated liquid than in the control group, suggesting that the dentin surface has a structure that can be easily penetrated by the resin and that the monomer acts on the dentin surface for a longer time than usual, which in turn promotes resin impregnation and reduces the water content in the dentin.…”