Staining for acid-fast bacilli in surgical pathology: practice patterns and variations

Fluorescent quantitative PCR detection ofMycobacterium tuberculosisin tissue sections from granulomatous lesions retrieved using EDTA

“…Targeted DNA of tissues could be ruptured and missed during the fixing process, which may lead to false-negative results 9. Other researchers believed that it might be related to tissue protein cross-linking during formalin fixation 6 7 10–13. High-temperature and high-pressure retrieval and EDTA heat-induced retrieval were the representative retrieval schemes for antigen retrieval in IHC, the principle of which is that the protein cross-linking induced by formaldehyde was broken by heating.…”

“…A total of 20 sections, 6 µm thick, were prepared from each, 10 of which were used for routine fluorescence quantitative PCR and another 10 for fluorescence quantitative PCR with EDTA heat-induced retrieval to detect TB/non-tuberculous mycobacteria. Another two serial sections were used for acid-fast staining and Auramine O staining, respectively, following the method described in previous studies 3 7…”

“…High-temperature and high-pressure retrieval and EDTA heat-mediated retrieval were the representative retrieval schemes for antigen retrieval in immunochemistry (IHC), the principle of which was that the protein cross-linking induced by formaldehyde was broken by heating. So, the antigen sites were exposed and contributed to the success of IHC 6 7 12 13…”

Mycobacterium tuberculosis Thymidylyltransferase RmlA Is Negatively Regulated by Ser/Thr Protein Kinase PknB

“…To further investigate the influence of the RmlA mutations to cell wall formation, we studied the effect of RmlA mutations to cell wall integrity by applying an acid-fast staining assay. It is reported that Mycobacteria contains a large amount of lipid in the cell wall, and once stained, the cell resists discoloration with dilute mineral acids (Wu et al, 2012). Compared with the WT, T12A mutant has a slight decrease in the remaining acid-fast, whereas T12D mutant was almost unable to retain the primary stain after washing with the acid-alcohol decolorizer (Figure 5C).…”

A New and Convenient Method to Get Paraffin-Embedded Sections of Tuberculous Bacteria as Positive Quality Controls for Acid-Fast Staining

“…Even though culture is considered as gold standard for the diagnosis, formalin-xed para n-embedded tissue with AFB staining was used as the initial diagnostic test in suspected cases of TB as it is a simple and less expensive outpatient diagnostic procedure. AFB staining of sputum smear samples has a long history in the diagnosis of TB, most commonly employing ZN stain, but the quality control of AFB in the tissue section is not perfect [4] .…”