Stages of in vitro phagocytosis of Plasmodium falciparum-infected erythrocytes by human monocytes

Muniz‐Junqueira, Maria Imaculada; Tosta, Carlos Eduardo

doi:10.1590/s0037-86822009000200001

Cited by 4 publications

(2 citation statements)

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“…Monocytes are released from bone marrow as undifferentiated cells to circulate in the blood for between 1 and 3 days [26]. They represent the early mononuclear phagocyte component of host defense and are the main cells responsible for the clearance of apoptotic bodies and infected erythrocytes [27,28].…”

Section: Monocytes and Their Luxury Oxygen Conditions In The Bloodmentioning

confidence: 99%

Hypoxia: how does the monocyte-macrophage system respond to changes in oxygen availability?

Strehl

Fangradt

Fearon

et al. 2013

Journal of Leukocyte Biology

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Hypoxia is an important feature of inflamed tissue, such as the RA joint. Activated monocytes/macrophages and endothelial cells play a pivotal role in the pathogenesis of RA, implicated in the mechanism of inflammation and erosion. During development, myeloid progenitor cells sequentially give rise to monoblasts, promonocytes, and monocytes that are released from the bone marrow into the bloodstream. After extravasation, monocytes differentiate into long-lived, tissue-specific macrophages or DCs. The effect of different oxygen concentrations experienced by these cells during maturation represents a novel aspect of this developmental process. In inflamed joint tissue, the microvascular architecture is highly dysregulated; thus, efficiency of oxygen supply to the synovium is poor. Therefore, invading cells must adapt instantaneously to changes in the oxygen level of the microenvironment. Angiogenesis is an early event in the inflammatory joint, which is important in enabling activated monocytes to enter via endothelial cells by active recruitment to expand the synovium into a "pannus", resulting in cartilage degradation and bone destruction. The increased metabolic turnover of the expanding synovial pannus outpaces the dysfunctional vascular supply, resulting in hypoxia. The abnormal bioenergetics of the microenvironment further promotes synovial cell invasiveness. In RA, joint hypoxia represents a potential threat to cell function and survival. Notably, oxygen availability is a crucial parameter in the cellular energy metabolism, itself an important factor in determining the function of immune cells.

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Section: Monocytes and Their Luxury Oxygen Conditions In The Bloodmentioning

confidence: 99%

Hypoxia: how does the monocyte-macrophage system respond to changes in oxygen availability?

Strehl

Fangradt

Fearon

et al. 2013

Journal of Leukocyte Biology

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show abstract

“…Os linfócitos ativados passam a produzir grande quantidade de anticorpos que ocupam os sítios de ligação nas células do hospdeiro impedindo a entrada do parasito nos hepatócitos e eritrócitos, como também facilitam a fagocitose. A ação dos anticorpos opsonizantes aumenta a adesão dos eritrócitos infectados pelo plasmódio aos monócitos, potencializando a fagocitose (Theander, 1991;Muniz-Junqueira e Tosta, 2009).…”

Section: Papel Da Imunidade Inata Na Defesa Contra O Plasmódiounclassified

Influência do tratamento com os antiplasmodiais artemeter, lumefantrina e mefloquina em funções dos monócitos envolvidas na defesa contra as formas eritrocitárias do Plasmodium falciparum

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Fagocitose de esquizontes quando avaliada pelos receptores para padrões moleculares de patógenos 4.3.1.1. Lumefantrina 4.3.1.2. Artemeter 4.3.1.3. Mefloquina 4.3.2. Fagocitose de esquizonte quando avaliada pelos receptores para opsoninas 4.3.2.1. Lumefantrina 4.3.2.2. Mefloquina 4.3.2.3. Artemeter 4.4.1. Fagocitose de parasitos na forma em anel pelos receptores para padrões moleculares para patógenos 4.4.1.1. Artemeter 4.4.1.2. Lumefantrina XI 4.4.1.3. Mefloquina 4.4.2. Fagocitose de parasitos na forma em anel pelos receptores para opsoninas 4.4.2.1. Artemeter 4.4.2.2. Lumefantrina 4.4.2.3. Mefloquina 4.5. Corpúsculos lipídicos 4.5.1. Influencia dos antimaláricos sobre a expressão corpúsculos lipídicos pelos monócitos quando estimulados por eritrócitos parasitados não sensibilizados 4.5.1.1. Artemeter 4.5.1.2. Lumefantrina 4.5.1.3. Mefloquina 4.5.2. Influência dos antimaláricos sobre a expressão de corpúsculos lipídicos por monócitos estimulados com eritrócitos infectados com P. falciparum sensibilizados 4.5.2.1. Artemeter 4.5.2.2. Lumefantrina 4.5.2.3. Mefloquina 4.6. Influência dos antimaláricos sobre a produção de óxido nítrico pelos monócitos estimulados ou não com eritrócitos infectados ou não com P. falciparum 4.7. Influência dos antimaláricos sobre a produção de peróxido de hidrogênio pelos monócitos estimulados ou não com eritrócitos infectados ou não com P. falciparum

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Further evaluation of the NWF filter for the purification of Plasmodium vivax-infected erythrocytes

Tao

et al. 2017

Malar J

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BackgroundIsolation of Plasmodium-infected red blood cells (iRBCs) from clinical blood samples is often required for experiments, such as ex vivo drug assays, in vitro invasion assays and genome sequencing. Current methods for removing white blood cells (WBCs) from malaria-infected blood are time-consuming or costly. A prototype non-woven fabric (NWF) filter was developed for the purification of iRBCs, which showed great efficiency for removing WBCs in a pilot study. Previous work was performed with prototype filters optimized for processing 5–10 mL of blood. With the commercialization of the filters, this study aims to evaluate the efficiency and suitability of the commercial NWF filter for the purification of Plasmodium vivax-infected RBCs in smaller volumes of blood and to compare its performance with that of Plasmodipur® filters.MethodsForty-three clinical P. vivax blood samples taken from symptomatic patients attending malaria clinics at the China–Myanmar border were processed using the NWF filters in a nearby field laboratory. The numbers of WBCs and iRBCs and morphology of P. vivax parasites in the blood samples before and after NWF filtration were compared. The viability of P. vivax parasites after filtration from 27 blood samples was examined by in vitro short-term culture. In addition, the effectiveness of the NWF filter for removing WBCs was compared with that of the Plasmodipur® filter in six P. vivax blood samples.ResultsFiltration of 1–2 mL of P. vivax-infected blood with the NWF filter removed 99.68% WBCs. The densities of total iRBCs, ring and trophozoite stages before and after filtration were not significantly different (P > 0.05). However, the recovery rates of schizont- and gametocyte-infected RBCs, which were minor parasite stages in the clinical samples, were relatively low. After filtration, the P. vivax parasites did not show apparent morphological changes. Culture of 27 P. vivax-infected blood samples after filtration showed that parasites successfully matured into the schizont stage. The WBC removal rates and iRBC recovery rates were not significantly different between the NWF and Plasmodipur® filters (P > 0.05).ConclusionsWhen tested with 1–2 mL of P. vivax-infected blood, the NWF filter could effectively remove WBCs and the recovery rates for ring- and trophozoite-iRBCs were high. P. vivax parasites after filtration could be successfully cultured in vitro to reach maturity. The performance of the NWF and Plasmodipur® filters for removing WBCs and recovering iRBCs was comparable.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-017-1855-3) contains supplementary material, which is available to authorized users.

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Stages of in vitro phagocytosis of Plasmodium falciparum-infected erythrocytes by human monocytes

Cited by 4 publications

References 13 publications

Hypoxia: how does the monocyte-macrophage system respond to changes in oxygen availability?

Hypoxia: how does the monocyte-macrophage system respond to changes in oxygen availability?

Influência do tratamento com os antiplasmodiais artemeter, lumefantrina e mefloquina em funções dos monócitos envolvidas na defesa contra as formas eritrocitárias do Plasmodium falciparum

Further evaluation of the NWF filter for the purification of Plasmodium vivax-infected erythrocytes

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