Staged cultivation enhances biomass accumulation in the green growth phase of Haematococcus pluvialis

Sun, Han; Bin, Liu; Lu, Xinli; Cheng, Ka‐Wing; Chen, Feng

doi:10.1016/j.biortech.2017.03.011

Cited by 56 publications

(36 citation statements)

References 37 publications

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“…This result was also observed in other studies about replacement cultivation [19,20,21]. Replacement with fresh medium not only supplied nutrients to promote the growth of L. bisecta SAG2043, but also removed the extracellular metabolites in the old medium, which may inhibit the cell growth or induce cell death produced in old medium [20]. During the course of culture, the lipid content of L. bisecta SAG2043 increased gradually ( Fig.…”

Section: Sag2043 the Biomass Under Fresh Medium Containing A High Orsupporting

confidence: 85%

Evaluation and transcriptome analysis of the novel oleaginous microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for arachidonic acid production

Gao¹,

Huang²,

Lei³

et al. 2019

Preprint

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Background Microalgae can synthesize and accumulate various components that can be used in health supplements, pharmaceuticals, cosmeceuticals, and other bioproducts. Arachidonic acid (AA) is an omega-6 long-chain polyunsaturated fatty acid and is important for human health. The coccoid green microalga Lobosphaera bisecta SAG2043 has been reported to be able to accumulate high AA content under certain conditions. We evaluated the interactive effects of nitrogen concentration, light intensity, and medium replacement on the growth and accumulation of lipids and AA in this strain. We further performed transcriptome analysis on L. bisecta in order to elucidate the mechanism of AA and triacylglycerol (TAG) metabolism under nitrogen deficiency. Results Nutrient management and light intensity had significant effects on the biomass and accumulation of lipids and AA in L. bisecta. Both a high nitrogen concentration (18 mM) and high light intensity (bilateral light-300 μmol m -2 s -1 ) were beneficial to the growth of L. bisecta, and the replacement of culture medium further enhanced the biomass, which eventually reached 8.9 g L -1 . Low nitrogen concentration (3.6 mM) and high light significantly promoted the accumulation of lipids and AA. The highest lipid and AA content reached 54.0% and 10.8% of dry weight, respectively. Lipid compositions analysis under low nitrogen concentration showed that neutral lipids comprised 86.5% of the total lipids, and 88.2% of AA was distributed within the neutral lipids. We then reconstructed the lipid metabolic pathways of L. bisecta for the first time, and demonstrated that upregulation of a key desaturase and elongase in the Δ6 pathway was conducive to the accumulation of fatty acids toward AA synthesis. The synthesis of oleic acid, especially, ensured an adequate supply of precursor for AA synthesis. In addition, crucial genes within the TAG synthesis pathway were also up-regulated. Conclusion L. bisecta SAG2043 exhibits rapid growth, and high lipid and AA production. It may be a potential candidate for AA production. Transcriptome analysis showed that multi-level regulation ensured the conversion efficiency from carbon to the synthesis of fatty acids, which significantly promoted the accumulation of AA and TAG in L. bisecta.

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Section: Sag2043 the Biomass Under Fresh Medium Containing A High Orsupporting

confidence: 85%

Evaluation and transcriptome analysis of the novel oleaginous microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for arachidonic acid production

Gao¹,

Huang²,

Lei³

et al. 2019

Preprint

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“…Sun et al. () showed that a mixture of red and white LEDs in the ratio 2:1 (by PFD) produced biomass concentrations as high as 0.161 g · L −1 . Therefore, the effect of light quality was investigated for the current strain.…”

Section: Resultsmentioning

confidence: 99%

“…Xi et al (2016) showed that wavelengths in the red range were favored for vegetative growth and those in the blue range for astaxanthin induction in their strain. Sun et al (2017) showed that a mixture of red and white LEDs in the ratio 2:1 (by PFD) produced biomass concentrations as high as 0.161 g Á L À1 . Therefore, the effect of light quality was investigated for the current strain.…”

Section: Resultsmentioning

confidence: 99%

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Mazumdar

Novis

Visnovsky

et al. 2019

Journal of Phycology

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The present study investigated the effect of different culture conditions on the vegetative growth of a new species, Haematococcus alpinus (strain LCR‐CC‐261f) using airlift photobioreactors. The influence of culture medium, aeration rates, CO2 concentration in air‐gas mixture, temperature, light intensities, and wavelengths were investigated to achieve sustainable high cell density cultures. Growth parameters were determined by fitting the data to a form of the logistic equation that included a lag phase. The shear‐sensitive vegetative cells favored lower aeration rates in the photobioreactors. MLA medium increased to 40 mM nitrate produced high density cultures. Temperatures between 12°C and 18°C, 3% (v/v) CO2 concentration and a narrow photon flux density ranging between 37 and 48 μmol photons · m−2 · s−1 were best suited for growth. The wavelength of the light source also impacted growth and a high cell density of 9.6 × 105 cells · mL−1 was achieved using a mixture of red and blue compared to warm white, red, or blue LEDs.

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“…Variations in light and nutrient would lead algal cells to grow at a steady-growth state or redistribute carbon sinks for survival. For instance, previous study stated sufficient nutrient preferably increased the biomass, while excess light and nitrogen starvation favored in the accumulation of lipid and carotenoid by elevating pyruvate availability 17,18 . Consider excess light has a great potential to promote accumulation of carotenoid and polyunsaturated fatty acids (PUFAs).…”

mentioning

confidence: 99%

“…On the other hand, nutrient is involved in carbon and nitrogen metabolism, where modes of repletion, starvation, recovery and recycle are considered as valid strategies for increasing the productivity of biomass and high-value products 16 . Furthermore, coordinating advantages of light and nutrient brings out various powerful strategies for microalgal cultivation such as light-increment culture, flashing-light culture, mixing-light culture, fed-batch culture, gradient-fed culture, semi-continuous culture, and nutrient-starvation stress culture [17][18][19][20] . However, since most reported cultivations are carried out in a black-box model through a single chemical reaction of nutrient into biomass, precision and adaptability of the strategies proposed above are limited in the realistic cultivation.…”

mentioning

confidence: 99%

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

et al. 2020

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Accumulation of high-value products in microalgae is not conducive with rapid cell growth, which is the potential conflict in microalgal production. Overcoming such conflict faces numerous challenges in comprehensively understanding cell behavior and metabolism. Here, we show a fully integrated interaction between cell behavior, carbon partitioning, carbon availability and path rate of central carbon metabolism, and have practically overcome the production conflict of Chromochloris zofingiensis. We demonstrate that elevated carbon availability and active path rate of precursors are determinants for product biosynthesis, and the former exhibits a superior potential. As protein content reaches a threshold value to confer survival advantages, carbon availability becomes the major limiting factor for product biosynthesis and cell reproduction. Based on integrated interaction, regulating the C/N balance by feeding carbon source under excess light increases content of high-value products without inhibiting cell growth. Our findings provide a new orientation to achieve great productivity improvements in microalgal production.

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Staged cultivation enhances biomass accumulation in the green growth phase of Haematococcus pluvialis

Cited by 56 publications

References 37 publications

Evaluation and transcriptome analysis of the novel oleaginous microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for arachidonic acid production

Evaluation and transcriptome analysis of the novel oleaginous microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for arachidonic acid production

Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

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