Stage specific transcriptome profiles at cardiac lineage commitment during cardiomyocyte differentiation from mouse and human pluripotent stem cells

Cho, Sung Woo; Kim, Hyoung Kyu; Sung, Ji Hee; Han, Jin

doi:10.5483/bmbrep.2021.54.9.046

Cited by 1 publication

(3 citation statements)

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“…In addition, we have previously shown the transcriptome profiles and the PPI networks at cardiac lineage commitment comparing with those of mesodermal cells and differentiated cardiomyocytes [ 3 ]. Similarly, in the present study, we performed MEM PPI analysis at cardiac lineage commitment and during cardiac maturation.…”

Section: Discussionmentioning

confidence: 99%

“…We applied the selected MEM genes to PPI networks at cardiac lineage commitment and during cardiac maturation from our previous study ( Supplementary Figs. 1 and 2 ) and to newly constructed MEM PPI networks [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…The process of cardiac differentiation of PSC-derived mesodermal cells generally involves cardiac lineage commitment and maturation. Cardiomyocyte proliferation and maturation occur simultaneously during the early stage of differentiation, including cardiac lineage commitment, whereas it decreases and the maturation process is dominant during the late stage of differentiation [ 3 , 4 ]. We previously reported a unique population of PSC-derived platelet-derived growth factor receptor-alpha (PDGFRα + ) cardiac lineage-committed cells (CLCs), which are morphologically and functionally immature but actively proliferating compared to differentiated cardiomyocytes [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial energy metabolic transcriptome profiles during cardiac differentiation from mouse and human pluripotent stem cells

Cho

Kim

Sung

et al. 2022

Korean J Physiol Pharmacol

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Simultaneous myofibril and mitochondrial development is crucial for the cardiac differentiation of pluripotent stem cells (PSCs). Specifically, mitochondrial energy metabolism (MEM) development in cardiomyocytes is essential for the beating function. Although previous studies have reported that MEM is correlated with cardiac differentiation, the process and timing of MEM regulation for cardiac differentiation remain poorly understood. Here, we performed transcriptome analysis of cells at specific stages of cardiac differentiation from mouse embryonic stem cells (mESCs) and human induced PSCs (hiPSCs). We selected MEM genes strongly upregulated at cardiac lineage commitment and in a time-dependent manner during cardiac maturation and identified the protein-protein interaction networks. Notably, MEM proteins were found to interact closely with cardiac maturation-related proteins rather than with cardiac lineage commitment-related proteins. Furthermore, MEM proteins were found to primarily interact with cardiac muscle contractile proteins rather than with cardiac transcription factors. We identified several candidate MEM regulatory genes involved in cardiac lineage commitment ( Cck, Bdnf, Fabp4, Cebp α , and Cdkn2a in mESC-derived cells, and CCK and NOS3 in hiPSC-derived cells) and cardiac maturation ( Ppargc1 α , Pgam2, Cox6a2, and Fabp3 in mESC-derived cells, and PGAM2 and SLC25A4 in hiPSC-derived cells). Therefore, our findings show the importance of MEM in cardiac maturation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%