Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici

Zhang, Yonghong; Qu, Zhibo; Zheng, Wenming; Liu, Bo; Wang, Xiaojie; Xue, Xiao‐Feng; Xu, Liangsheng; Huang, Lili; Han, Qingmei; Zhao, Jie; Kang, Zhensheng

doi:10.1186/1471-2164-9-203

Cited by 54 publications

(72 citation statements)

References 56 publications

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“…Protein phosphorylation can be conducted by enzymes such as protein kinase C, cyclic AMP (cAMP)-dependent protein kinase, mitogen-activated protein (MAP) kinase, and type 1, 2A, and 2B serine/threonine phosphatases. Clones representing transcripts of some of these enzymes have been found in multiple cDNA libraries from filamentous fungi (Sacadura and Saville, 2003;Zhang et al, 2008). Our results indicate that Contig10 has similarity with a serine/threonine protein phosphatase PP1 from Ajellomyces capsulatus, while Contig41 encodes a serine/threonine protein kinase domain.…”

Section: Resultsmentioning

confidence: 67%

“…Considering that this library is one of the few P. pachyrhizi cDNA libraries that have been analyzed thus far, our results demonstrate that a different gene set is expressed in uredinium compared to germinating spores. The infection process includes three major steps: germination and penetration, proliferation and sporulation (Solomon et al, 2003;Zhang et al, 2008). At 240 hai, sporulation occurs.…”

Section: Resultsmentioning

confidence: 99%

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Soybean Rust: Five Years of Research

Tremblay¹

2011

Soybean - Molecular Aspects of Breeding

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Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Soybean Rust: Five Years of Research

Tremblay¹

2011

Soybean - Molecular Aspects of Breeding

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“…Furthermore, the ability of Pst to form new races that can attack previously resistant cultivars, along with the capacity of fungal spores to travel long distances, can make control of stripe rust difficult. Over the last few years, epidemiological [13,14], genetic [15][16][17][18], histological [19] and molecular [20][21][22][23] studies on the disease and pathogen have been reported. The wheat-Pst interactions have been studied at the molecular genetics and ultrastructural levels [24,25].…”

Section: Introductionmentioning

confidence: 99%

- Strategies of Nitrosomonas europaea 19718 to Counter Low Dissolved Oxygen and High Nitrite Concentrations

2016

Phytopathology in Plants

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Background: Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Due to special features of hexaploid wheat with large and complex genome and difficulties for transformation, and of Pst without sexual reproduction and hard to culture on media, the use of most genetic and molecular techniques in studying genes involved in the wheat-Pst interactions has been largely limited. The objective of this study was to identify transcriptionally regulated genes during an incompatible interaction between wheat and Pst using cDNA-AFLP technique Results: A total of 52,992 transcript derived fragments (TDFs) were generated with 64 primer pairs and 2,437 (4.6%) of them displayed altered expression patterns after inoculation with 1,787 up-regulated and 650 down-regulated. We obtained reliable sequences (>100 bp) for 255 selected TDFs, of which 113 (44.3%) had putative functions identified. A large group (17.6%) of these genes shared high homology with genes involved in metabolism and photosynthesis; 13.8% to genes with functions related to disease defense and signal transduction; and those in the remaining groups (12.9%) to genes involved in transcription, transport processes, protein metabolism, and cell structure, respectively. Through comparing TDFs identified in the present study for incompatible interaction and those identified in the previous study for compatible interactions, 161 TDFs were shared by both interactions, 94 were expressed specifically in the incompatible interaction, of which the specificity of 43 selected transcripts were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Based on the analyses of homology to genes known to play a role in defense, signal transduction and protein metabolism, 20 TDFs were chosen and their expression patterns revealed by the cDNA-AFLP technique were confirmed using the qRT-PCR analysis. Conclusion: We uncovered a number of new candidate genes possibly involved in the interactions of wheat and Pst, of which 11 TDFs expressed specifically in the incompatible interaction. Resistance to stripe rust in wheat cv. Suwon11 is executed after penetration has occurred. Moreover, we also found that plant responses in compatible and incompatible interactions are qualitatively similar but quantitatively different soon after stripe rust fungus infection.

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“…Stage-specific genes have been identified in diverse fungal species including Fusarium graminearum (Seong et al, 2008) and Puccinia striiformis f. sp. tritici (Zhang et al, 2008). Multiple genes specific to conidiation have been identified in Neurospora crassa (Springer & Yanofsky, 1992) and Aspergillus nidulans (Adams et al, 1998), such as con10 (N. crassa) and stuA (Asp.…”

Section: Introductionmentioning

confidence: 99%

Identification of growth stage molecular markers in Trichoderma sp. ‘atroviride type B’ and their potential application in monitoring fungal growth and development in soil

et al. 2015

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Several members of the genus Trichoderma are biocontrol agents of soil-borne fungal plant pathogens. The effectiveness of biocontrol agents depends heavily on how they perform in the complex field environment. Therefore, the ability to monitor and track Trichoderma within the environment is essential to understanding biocontrol efficacy. The objectives of this work were to: (a) identify key genes involved in Trichoderma sp. 'atroviride type B' morphogenesis; (b) develop a robust RNA isolation method from soil; and (c) develop molecular marker assays for characterizing morphogenesis whilst in the soil environment. Four cDNA libraries corresponding to conidia, germination, vegetative growth and conidiogenesis were created, and the genes identified by sequencing. Stage specificity of the different genes was confirmed by either Northern blot or quantitative reverse-transcriptase PCR (qRT-PCR) analysis using RNA from the four stages. con10, a conidial-specific gene, was observed in conidia, as well as one gene also involved in subsequent stages of germination (L-lactate/malate dehydrogenase encoding gene). The germination stage revealed high expression rates of genes involved in amino acid and protein biosynthesis, while in the vegetative-growth stage, genes involved in differentiation, including the mitogen-activated protein kinase kinase similar to Kpp7 from Ustilago maydis and the orthologue to stuA from Aspergillus nidulans, were preferentially expressed. Genes involved in cell-wall synthesis were expressed during conidiogenesis. We standardized total RNA isolation from Trichoderma sp. 'atroviride type B' growing in soil and then examined the expression profiles of selected genes using qRT-PCR. The results suggested that the relative expression patterns were cyclic and not accumulative.

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Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici

Cited by 54 publications

References 56 publications

Soybean Rust: Five Years of Research

Soybean Rust: Five Years of Research

- Strategies of Nitrosomonas europaea 19718 to Counter Low Dissolved Oxygen and High Nitrite Concentrations

Identification of growth stage molecular markers in Trichoderma sp. ‘atroviride type B’ and their potential application in monitoring fungal growth and development in soil

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