Stage-related expression of mRNAs during pollen development in lily and tobacco

Schrauwen, J. A. M.; Groot, P. F. M. de; Herpen, M. M. A. van; Lee, T. van der; Reynen, W. H.; Weterings, Koen; Wullems, G. J.

doi:10.1007/bf00197125

Cited by 73 publications

(44 citation statements)

References 23 publications

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“…Flower buds of selected length were collected to obtain pollen of different developmental stages. The determination of developmental stage and isolation of the anther content was carried out as previously described [27].…”

Section: Plant Materialsmentioning

confidence: 99%

“…By use of a light microscope, the selected pollen developmental stages are distinguished clearly by their morphological characteristics visible after a DAPI staining [27].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…The early developmental stage starts after meiosis and ends with the microspore mitosis, the late stage covers the period after mitosis up to mature pollen [ 14]. During pollen development the mRNA content present in different stages changes qualitatively [27]. Genes which are transiently and specifically transcribed during pollen development are assumed to play a crucial role in the developmental processes.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

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The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM 19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM 19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.

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Section: Plant Materialsmentioning

confidence: 99%

“…By use of a light microscope, the selected pollen developmental stages are distinguished clearly by their morphological characteristics visible after a DAPI staining [27].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

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“…During the formation o f pollen grains, specific genes are transcribed at the different stages of development (Schrauwen et al 1990;Mascarenhas 1992). To study the functional significance of pollen-specific genes, we analyzed the NTP303 gene o f Nicoiianci tcibacuni that is transcribed only in pollen (Weterings et al 1992).…”

Section: Introductionmentioning

confidence: 99%

The promoter of a gene that is expressed only in pollen interacts with ubiquitous transcription factors

Hochstenbach

Groot

Jacobs

et al. 1996

Sexual Plant Reprod

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The NTP303 gene of tobacco is expressed on ly in biecllular pollen. The minimal pollen-specific pro moter of the gene is located between -103 and -51 up stream of the transcriptional start site. Using gel mobility shift assays, we demonstrated that this minimal function al promoter interacts with a leaf nuclear GT-l binding activity. This finding suggests that ubiquitous transcrip tion factors are involved in the pollen-specific expression of the gene in vivo.

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“…During pollen development, a large number of genes are transcribed (Willing and Mascarenhas, 1984;Willing et al, 1988;Guyon et al, 2000;F. Cnudde, unpublished data), which leads to the appearance of distinctive sets of transcripts (Stinson et al, 1987;Schrauwen et al, 1990;Hulzink, 2002). These mRNA sets can be found in pollen from both mono-and dicotyledonous plant species, which argues for a conservation of genetic programs that underlie pollen gene expression.…”

mentioning

confidence: 99%

In Silico Identification of Putative Regulatory Sequence Elements in the 5′-Untranslated Region of Genes That Are Expressed during Male Gametogenesis

et al. 2003

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During pollen development, transcription of a large number of genes results in the appearance of distinct sets of transcripts. Similar mRNA sets are present in pollen of both mono-and dicotyledonous plant species, which indicates an evolutionary conservation of genetic programs that determine pollen gene expression. In pollen, regulation of gene expression occurs at the transcriptional and posttranscriptional level. The 5Ј-untranslated region (UTR) of several pollen transcripts has been shown to be important for regulation of pollen gene expression. The important regulatory role of 5Ј-UTR sequences and the evolutionary conservation of genetic programs in pollen led to the hypothesis that the 5Ј-UTRs of pollen-expressed genes share regulatory sequence elements. In an attempt to identify these pollen 5Ј-UTR elements, a statistical analysis was performed using 5Ј-UTR sequences of pollen-and sporophytic-expressed genes. The analysis revealed the presence of several pollen-specific 5Ј-UTR sequence elements. Assembly of the pollen 5Ј-UTR elements led to the identification of various consensus sequences, including those that previously have been demonstrated to play a role in the regulation of pollen gene expression. Several pollen 5Ј-UTR elements were found to be preferentially associated to genes from dicots, wet-type stigma plants, or plants containing bicellular pollen. Moreover, three sequence elements exhibited a preferential association to the 5Ј-UTR of pollen-expressed genes from Arabidopsis and Brassica napus. Functional implications of these observations are discussed.

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Stage-related expression of mRNAs during pollen development in lily and tobacco

Cited by 73 publications

References 23 publications

Isolation and characterization of a microspore-specific gene from tobacco

Isolation and characterization of a microspore-specific gene from tobacco

The promoter of a gene that is expressed only in pollen interacts with ubiquitous transcription factors

In Silico Identification of Putative Regulatory Sequence Elements in the 5′-Untranslated Region of Genes That Are Expressed during Male Gametogenesis

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