Stage and organ specificity of the degree of variegation of the 6-phosphogluconate dehydrogenase gene in Drosophila melanogaster

Slobodyanyuk, S. Ya.; Serov, O. L.

doi:10.1007/bf00330461

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…The product of the Pgd gene, 6-phosphogluconate dehydrogenase, can be assayed quantitatively in various tissues. Slobodyanyuk and Serov [1987] investigated the effects of several rearrangements displaying position-effect variegations that suppressed the activity of this gene at different stages of Drosophila development. Although the degree of variegation of Pgd was higher in adult than larval tissues, position-effect variegation was almost absent in the imago ovaries.…”

Section: Discussionmentioning

confidence: 99%

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Nurse cell polytene chromosomes ofDrosophila melanogaster otu mutants: Morphological changes accompanying interallelic complementation and position effect variegation

et al. 1997

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Combinations of certain mutant alleles of the ovarian tumor gene permit the production of viable eggs. Two alleles that behave in this way are otu7 and otu1. Females homozygous for either allele are sterile, and their ovarian nurse cells (NC) contain giant polytene chromosomes of various morphologies. Fertile flies (otu+ / otu+, otu− / otu7, otu+ / otu11) have endopolyploid nurse cells with typical dispersed chromosomes. Fertile hybrids (otu7 / otu11) produce large numbers of polytene chromosomes comparable to, and often larger than, classic salivary gland (SG) chromosomes. Therefore, these otu hybrids provide a unique system for studying, at the chromosomal level, the activation and expression of genes functioning during oogenesis. The otu gene encodes a long and a short isoform. The normal long isoform appears to be responsible for the dispersion of chromosomes during the endomitotic DNA replications occurring in ovarian NCs. The genetic inactivation of euchromatic genes placed next to pericentric heterochromatin by a chromosomal rearrangement is accompanied by the compaction of corresponding chromosome regions. A comparative study of the manifestation of position‐effect variegation for the polytene chromosomes of SG cells and NCs was made using the Dp(1;1)pn2b and Dp(1;f)1337 rearrangements. The percentage frequencies of block formation in the SG and NC nuclei for Dp (1;1) pn2b rearrangement were 92.6% vs. 15.8%, respectively; for Dp(1;f) 1337, these values were 56.8% vs. 9.7%. Therefore heterochromatin belonging to germ line chromosomes is in a configuration that is far less likely to inactivate inserted segments of euchromatin than is heterachromatin from somatic chromosomes. Dev. Genet. 20:163–174. 1997. © 1997 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

“…In other words, the inactivated euchromatic region turns into a dense block of compact, late-replicating chromatin [Zhimulev et al, 1989;Belyaeva and Zhimulev, 1991]. In contrast to larval SGs, no suppression of relocated euchromatic genes was found in the ovaries of adult flies [Slobodyanyuk and Serov, 1987].…”

Section: Introductionmentioning

confidence: 99%

Nurse cell polytene chromosomes ofDrosophila melanogaster otu mutants: Morphological changes accompanying interallelic complementation and position effect variegation

et al. 1997

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Extent of polyteny in the pericentric heterochromatin of polytene chromosomes of pseudonurse cells of otu (ovarian tumor) mutants of Drosophila melanogaster

Mal'ceva

Жимулев

1993

Molec. Gen. Genet.

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In the polytene nuclei of germ-line cells (ovarian pseudonurse cells) of Drosophila melanogaster females mutant for otu11 (ovarian tumor), the pericentric heterochromatin is much more abundant than in somatic salivary gland cells. This is due to the degree of heterochromatin compaction (and consequently the level of underreplication) being lower in the nurse cells than in the salivary gland cells. The lower level of compaction probably results in a very low degree of position effect gene inactivation in the ovarian nurse cells.

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Polytene Chromosomes, Heterochromatin, and Position Effect Variegation

Жимулев

1997

Advances in Genetics

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Stage and organ specificity of the degree of variegation of the 6-phosphogluconate dehydrogenase gene in Drosophila melanogaster

Cited by 4 publications

References 7 publications

Nurse cell polytene chromosomes ofDrosophila melanogaster otu mutants: Morphological changes accompanying interallelic complementation and position effect variegation

Nurse cell polytene chromosomes ofDrosophila melanogaster otu mutants: Morphological changes accompanying interallelic complementation and position effect variegation

Extent of polyteny in the pericentric heterochromatin of polytene chromosomes of pseudonurse cells of otu (ovarian tumor) mutants of Drosophila melanogaster

Polytene Chromosomes, Heterochromatin, and Position Effect Variegation

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