“…[4][5][6] Among the current technologies for gene transfer of leukemia cells, the latest generation of HIV-1-based lentiviral vectors represent the most efficient tool for their reported ability to transduce with stability, a wide variety of cell types in significant percentages. [7][8][9][10][11][12][13][14][15][16][17] B-cell precursor (BCP) blasts are very fragile and difficult to maintain alive in culture conditions. 18 The best available results so far for gene transduction have been obtained with second-generation lentiviral vectors monitoring the expression of the CD80 transgene up to 48 h. 5 More recently, second-generation lentiviral vectors have been further modified by deleting a portion of the U3 LTR region in the so-called self-inactivating vector (SIN) with improved safety, 8,9,15,19,20 and by the introduction of two cis-acting elements, one derived from the pol sequence, called central polypurine tract sequence (cPPT), 11,21,22 and the other, termed Wpre, obtained from the genome of the woodchuck hepatitis virus, both believed to increase transgene expression.…”