Stable star polymer nanolayers and their thermoresponsiveness as a tool for controlled culture and detachment of fibroblast sheets

Mendrek, Barbara; Żymełka-Miara, Iwona; Sieroń, Łukasz; Fus, Agnieszka; Balin, Katarzyna; Kubacki, Jerzy; Smet, Mario; Trzebicka, Barbara; Sieroń, Aleksander; Kowalczuk, Agnieszka

doi:10.1039/c7tb02748f

Cited by 12 publications

(13 citation statements)

References 34 publications

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“…Based on the obtained values and on the hydrodynamic diameters of the star polymers in acetone, which were in the range of 20-30 nm [60], it could be concluded that stars were formed not as monolayers, but rather in the form of multilayers. A similar situation was observed for layers made of stars with poly[oligo(ethylene glycol) methacrylate] arms where the obtained layer thickness made of the star polymer was in the range of 58-59.5 nm [59].…”

Section: Layersupporting

confidence: 78%

“…These signals were better visible at deconvoluted lines of XPS spectra (Supporting Information, Figure S1A,B). Bromine atoms originated from the PArOx core and from the ends of the arms in case of star layers and only from the ends of PDMAEMA chains for linear layers [59]. For comparison, the benzophenone functionalized layers (sample SBPH) did not show the presence of bromine at all (Supporting Information, Figure S1C).…”

Section: Physicochemical Characterization Of the Obtained Pdmaema Layersmentioning

confidence: 99%

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Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity

et al. 2020

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In this paper, we focus on the synthesis and characterization of novel stable nanolayers made of star methacrylate polymers. The effect of nanolayer modification on its antibacterial properties was also studied. A covalent immobilization of star poly(N,N′-dimethylaminoethyl methacrylate) (PDMAEMA) to benzophenone functionalized glass or silicon supports was carried out via a “grafting to” approach using UV irradiation. To date, star polymer UV immobilization has never been used for this purpose. The thickness of the resulting nanolayers increased from 30 to 120 nm with the molar mass of the immobilized stars. The successful bonding of star PDMAEMA to the supports was confirmed by surface sensitive quantitative spectroscopic methods. Next, amino groups in the polymer layer were quaternized with bromoethane, and the influence of this modification on the antibacterial properties of the obtained materials was analyzed using a selected reference strain of bacteria. The resulting star nanolayer surfaces exhibited higher antimicrobial activity against Bacillus subtilis ATCC 6633 compared to that of the linear PDMAEMA analogues grafted onto a support. These promising results and the knowledge about the influence of the topology and modification of PDMAEMA layers on their properties may help in searching for new materials for antimicrobial applications in medicine.

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Section: Layersupporting

confidence: 78%

Section: Physicochemical Characterization Of the Obtained Pdmaema Layersmentioning

confidence: 99%

Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity

et al. 2020

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“…The cell sheets can then be used as 2D building blocks in tissue engineering and regenerative medicine [ 6 , 7 ]. Besides extensively discussed and investigated poly( N -isopropyl acrylamide) (PNIPAm) gel [ 8 ] and brush [ 9 ] coatings, thermoresponsive brushes based on poly(oxazoline)s (POx) [ 10 , 11 ], poly[oligo(ethylene glycol) methacrylate]s (POEGMAs) [ 12 , 13 , 14 ] and poly(glycidyl ether)s (PGEs) [ 15 , 16 ] have shown to be among the most suitable thermoresponsive coatings for cell sheet fabrication.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, such membranes can also be exploited for the direct transfer of detaching cell sheets onto in vitro tissue culture scaffolds or for the regeneration of tissues in vivo. Whereas both “grafting-from” and “grafting-to” methods have been used to manufacture thermoresponsive PNIPAm coatings on most of the common culture substrate materials, reports on promising POx and POEGMA brush coatings are still mainly limited to glass substrates [ 10 , 11 , 12 , 13 , 14 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Stöbener

Weinhart

2020

Polymers

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Thermoresponsive poly(glycidyl ether) brushes can be grafted to applied tissue culture substrates and used for the fabrication of primary human cell sheets. The self-assembly of such brushes is achieved via the directed physical adsorption and subsequent UV immobilization of block copolymers equipped with a short, photo-reactive benzophenone-based anchor block. Depending on the chemistry and hydrophobicity of the benzophenone anchor, we demonstrate that such block copolymers exhibit distinct thermoresponsive properties and aggregation behaviors in water. Independent on the block copolymer composition, we developed a versatile grafting-to process which allows the fabrication of poly(glycidyl ether) brushes on various tissue culture substrates from dilute aqueous-ethanolic solution. The viability of this process crucially depends on the chemistry and hydrophobicity of, both, benzophenone-based anchor block and substrate material. Utilizing these insights, we were able to manufacture thermoresponsive poly(glycidyl ether) brushes on moderately hydrophobic polystyrene and polycarbonate as well as on rather hydrophilic polyethylene terephthalate and tissue culture-treated polystyrene substrates. We further show that the temperature-dependent switchability of the brush coatings is not only dependent on the cloud point temperature of the block copolymers, but also markedly governed by the hydrophobicity of the surface-bound benzophenone anchor and the subjacent substrate material. Our findings demonstrate that the design of amphiphilic thermoresponsive block copolymers is crucial for their phase transition characteristics in solution and on surfaces.

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“…In cell engineering, thermoresponsive polymers based on oligo(ethylene glycol) methacrylates [111][112][113][114][115][116], oxazolines [117][118][119][120], and ethers [121,122] were also applied.…”

Section: Substrates and Scaffolds For Cell And Tissue Culturementioning

confidence: 99%

Polymers in medicine direction of development

et al. 2019

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The paper constitutes a brief and subjective review of polymeric materials in the contemporary health service. The range of applications of polymeric materials is discussed, special att ention being paid to such materials for the development of carriers of pharmaceutically active species, stents and vascular prostheses, amongst them to the application of "smart" materials for these purposes, layers and scaff olds for the growth of organs and tissues, antifouling layers. The authors try to turn the att ention of the reader to the research and intellectual eff orts necessary for the development of polymeric materials for the medicine, and conclude about the growing importance of such studies.

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Stable star polymer nanolayers and their thermoresponsiveness as a tool for controlled culture and detachment of fibroblast sheets

Abstract: Star polymer nanolayers were successfully used as surfaces for fibroblast adhesion and proliferation, followed by their detachment in the form of a cell sheet, controlled by a temperature decrease.

Cited by 12 publications

References 34 publications

Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity

Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Polymers in medicine direction of development

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