Stable self-assembled nanostructured hen egg white lysozyme exhibits strong anti-proliferative activity against breast cancer cells

Mahanta, Sailendra; Paul, Subhankar; Srivastava, Ankit; Pastor, Ashutosh; Kundu, Bishwajit; Chaudhuri, Tapan K.

doi:10.1016/j.colsurfb.2015.04.017

Cited by 54 publications

(30 citation statements)

References 32 publications

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“…[32] Some of them, such as the bovine α-lactalbumin [33] or the hen egg white lysozyme, [34] have already proved their utility as the basis of wide spectrum antitumoral drugs. [32] Some of them, such as the bovine α-lactalbumin [33] or the hen egg white lysozyme, [34] have already proved their utility as the basis of wide spectrum antitumoral drugs.…”

Section: Resultsmentioning

confidence: 99%

Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

Pesarrodona

Jauset²,

Díaz‐Riascos

et al. 2019

Advanced Science

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Two structurally and functionally unrelated proteins, namely Omomyc and p31, are engineered as CD44‐targeted inclusion bodies produced in recombinant bacteria. In this unusual particulate form, both types of protein materials selectively penetrate and kill CD44+ tumor cells in culture, and upon local administration, promote destruction of tumoral tissue in orthotropic mouse models of human breast cancer. These findings support the concept of bacterial inclusion bodies as versatile protein materials suitable for application in chronic diseases that, like cancer, can benefit from a local slow release of therapeutic proteins.

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Section: Resultsmentioning

confidence: 99%

Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

Pesarrodona

Jauset²,

Díaz‐Riascos

et al. 2019

Advanced Science

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“…Lysozyme directly activates immune cells and increases tumor cell immunogenicity [ 22 ], which have been confirmed in a number of experimental cases [ 22 - 24 ]. However, even without immune cell activation, self-assembled nanostructured hen egg white lysozyme and recombinant human lysozyme showed strong antiproliferative effects in breast [ 23 ] and gastric [ 24 ] cancer cells, respectively. However, a better understanding of the antitumor mechanisms of lysozyme (intracellular or extracellular) is needed.…”

Section: Discussionmentioning

confidence: 99%

Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation

Yoo

Yeo

2017

Radiat Oncol J

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PurposeThe serum carcinoembryonic antigen (CEA) level has been recognized as a prognostic factor in colorectal cancer, and associated with response of rectal cancer to radiotherapy. This study aimed to identify CEA-interacting proteins in colon cancer cells and observe post-irradiation changes in their expression.Materials and MethodsCEA expression in colon cancer cells was examined by Western blot analysis. Using an anti-CEA antibody or IgG as a negative control, immunoprecipitation was performed in colon cancer cell lysates. CEA and IgG immunoprecipitates were used for liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis. Proteins identified in the CEA immunoprecipitates but not in the IgG immunoprecipitates were selected as CEA-interacting proteins. After radiation treatment, changes in expression of CEA-interacting proteins were monitored by Western blot analysis.ResultsCEA expression was higher in SNU-81 cells compared with LoVo cells. The membrane localization of CEA limited the immunoprecipitation results and thus the number of CEA-interacting proteins identified. Only the Ras-related protein Rab-6B and lysozyme C were identified as CEA-interacting proteins in LoVo and SNU-81 cells, respectively. Lysozyme C was detected only in SNU-81, and CEA expression was differently regulated in two cell lines; it was down-regulated in LoVo but up-regulated in SNU-81 in radiation dosage-dependent manner.ConclusionCEA-mediated radiation response appears to vary, depending on the characteristics of individual cancer cells. The lysozyme C and Rab subfamily proteins may play a role in the link between CEA and tumor response to radiation, although further studies are needed to clarify functional roles of the identified proteins.

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“…Oppositely, the antimicrobial efficiency of lysozyme against the Gram-negative bacteria is limited and even lacks toward eukaryotic cell walls [82]. Additionally, there are studies that reveal the potential of lysozyme to inhibit tumor formation and growth (anticancer agent) [85,86].…”

Section: Lysozymementioning

confidence: 99%

Protected Laser Evaporation/Ablation and Deposition of Organic/Biological Materials: Thin Films Deposition for Nano- biomedical Applications

Popescu-Pelin¹,

Ristoscu²,

Badiceanu³

et al. 2017

Laser Ablation - From Fundamentals to Applications

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This chapter reviews the laser ablation of delicate organic/biological substances by matrix-assisted pulsed laser evaporation (MAPLE). It is shown that direct ablation in this case is possible but sometimes not workable at all in adverse conditions. The considered solution is the protection by a prevalent dissolving/suspending component that can allow for a "shielded" ablation by the frozen solvent followed by its gradual evaporation by melting, evaporation and evacuation by pumping system. We extend the study to the case of non-UV absorbing solvents, e.g., water, when the primary interaction between laser and solute ignites evaporation process at a lower ablation threshold due to reduced pressure inside irradiation chamber. We called this case as "generalized" MAPLE interaction. Relevant examples are provided and critically analyzed in view of potential applications for nanobiomedicine, biosensors, advanced implants and chemical technologies.

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Stable self-assembled nanostructured hen egg white lysozyme exhibits strong anti-proliferative activity against breast cancer cells

Cited by 54 publications

References 32 publications

Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation

Protected Laser Evaporation/Ablation and Deposition of Organic/Biological Materials: Thin Films Deposition for Nano- biomedical Applications

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