Stable release of BDNF from the fibroblast cell line NIH3T3 grown on silicone elastomers enhances survival of spiral ganglion cells in vitro and in vivo

Warnecke, Athanasia; Sasse, S.; Wenzel, Gentiana I.; Hoffmann, Andrea; Groß, Gerhard; Paasche, Gerrit; Scheper, Verena; Reich, Uta; Esser, Karl‐Heinz; Lenarz, Thomas; Stöver, Timo; Wissel, Kirsten

doi:10.1016/j.heares.2012.04.007

Cited by 48 publications

(50 citation statements)

References 66 publications

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“…In vitro results proof a neuroprotective effect of NIH3T3 cell-derived BDNF (10). First, in vivo experiments with BDNF expressing NIH3T3 fibroblasts cultivated on the surface of silicone, the material used for CI electrode embedding, are promising (11). However, a partial destruction and delamination of BDNF-fibroblasts from the silicone surface, based on the mechanical forces during the implantation process, were observed.…”

mentioning

confidence: 77%

UHV-Alginate as Matrix for Neurotrophic Factor Producing Cells—A Novel Biomaterial for Cochlear Implant Optimization to Preserve Inner Ear Neurons From Degeneration

Hütten¹,

Erhacrt²,

Zimmermann³

et al. 2013

Otology &Amp; Neurotology

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confidence: 77%

UHV-Alginate as Matrix for Neurotrophic Factor Producing Cells—A Novel Biomaterial for Cochlear Implant Optimization to Preserve Inner Ear Neurons From Degeneration

Hütten¹,

Erhacrt²,

Zimmermann³

et al. 2013

Otology &Amp; Neurotology

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“…Spiral ganglion cells were dissected from the cochleae of decapitated P3 -P6 neonatal Sprague-Dawley rats of both sexes and prepared as previously described [16]. The procedure was approved by the Laboratory Animal Science Center of the Hannover Medical School and conducted in accordance with the German 'Law on Protecting Animals' ( §4/03 TierSchG) and with the European Communities Council Directive 2010/63/EU.…”

Section: Spiral Ganglion Cell Preparation and Cultivationmentioning

confidence: 99%

Nanosecond laser pulse stimulation of spiral ganglion neurons and model cells

Rettenmaier

Lenarz

Reuter

2014

Biomed. Opt. Express

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Optical stimulation of the inner ear has recently attracted attention, suggesting a higher frequency resolution compared to electrical cochlear implants due to its high spatial stimulation selectivity. Although the feasibility of the effect is shown in multiple in vivo experiments, the stimulation mechanism remains open to discussion. Here we investigate in single-cell measurements the reaction of spiral ganglion neurons and model cells to irradiation with a nanosecond-pulsed laser beam over a broad wavelength range from 420 nm up to 1950 nm using the patch clamp technique. Cell reactions were wavelength-and pulse-energy-dependent but too small to elicit action potentials in the investigated spiral ganglion neurons. As the applied radiant exposure was much higher than the reported threshold for in vivo experiments in the same laser regime, we conclude that in a stimulation paradigm with nanosecond-pulses, direct neuronal stimulation is not the main cause of optical cochlea stimulation.

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“…Cell-based approaches have been suggested for a sustained neurotrophin supply. For example, xenogenic choroid plexus cells [2] and genetically modified fibroblasts [5] have been implanted into the scala tympani. Adipose-derived stem cells (ASCs), multipotent stem cells that can be harvested from adult individuals, are also known to produce neurotrophins [6] and can be used autologously, thereby being more suitable for the application in patients.…”

Section: Introductionmentioning

confidence: 99%

Plasma-Assisted Hydrophilization of Cochlear Implant Electrode Array Surfaces Enables Adhesion of Neurotrophin-Secreting Cells

Schendzielorz

Schmitz²,

Moseke³

et al. 2014

ORL

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Objective: To evaluate plasma treatment for enhancing the biocompatibility of cochlear implant (CI) silicone surfaces, thus allowing colonization with human adipose-derived stem cells (hASCs) that are known to provide neurotrophic support. Methods: Silicone samples and CI electrode arrays were treated with 4 low-pressure plasmas of different characteristics. The hydrophilicity of plasma-treated and control surfaces as well as the adherence and morphology of hASCs were assessed. Finally, the insertion forces of electrode arrays were determined and the colonization potential of the electrode arrays with hASCs were tested. Results: The hydrophilicity of the silicone surfaces was significantly enhanced after plasma treatment, as was the adherence of hASCs. The characteristic morphology of hASCs was observed when grown on plasma-treated but not on untreated silicone surfaces. The insertion forces of plasma-treated electrode arrays were similar to those of untreated arrays, and the colonization of plasma-treated electrode arrays with hASCs was feasible. Conclusion: Plasma treatment of CI electrode arrays enhances their biocompatibility and allows for the colonization with hASCs that are known to produce neurotrophic factors. i 2014 S. Karger AG, Basel

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Stable release of BDNF from the fibroblast cell line NIH3T3 grown on silicone elastomers enhances survival of spiral ganglion cells in vitro and in vivo

Cited by 48 publications

References 66 publications

UHV-Alginate as Matrix for Neurotrophic Factor Producing Cells—A Novel Biomaterial for Cochlear Implant Optimization to Preserve Inner Ear Neurons From Degeneration

UHV-Alginate as Matrix for Neurotrophic Factor Producing Cells—A Novel Biomaterial for Cochlear Implant Optimization to Preserve Inner Ear Neurons From Degeneration

Nanosecond laser pulse stimulation of spiral ganglion neurons and model cells

Plasma-Assisted Hydrophilization of Cochlear Implant Electrode Array Surfaces Enables Adhesion of Neurotrophin-Secreting Cells

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