Stable plasma-deposited acrylic acid surfaces for cell culture applications

Conformal poly(allyl alcohol) (PAA) coatings were grown on a biomedical grade polyurethane scaffold using pulsed plasma polymerization of the allyl alcohol monomer. The creation of a continuous wave polymer primer layer increases the interfacial adhesion and stability of a subsequent pulsed plasma deposited PAA film. The resulting PAA coatings are strongly hydrophilic and remain unchanged following seven days incubation in biological media. Films prepared through this two-step process promote human dermal fibroblast cell culture, while resisting E. Coli biofilm formation.

show abstract

“…Plasma polymer coatings of acrylic acid [27], isopropyl alcohol [28], allylamine [Error! Bookmark not defined.…”

Section: Introductionmentioning

confidence: 99%

Plasma-Generated Poly(allyl alcohol) Antifouling Coatings for Cellular Attachment

Watkins¹,

Lee

Moir³

et al. 2016

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Surfaces containing this coating have been reported in applications ranging from platelet adhesion promotion [13], RGD peptide immobilisation [14], attachment of osteoblast-like [15], fibroblast [16] and keratinocyte [17] cells as well as collagen molecule grafting [18].…”

Section: Introductionmentioning

confidence: 99%

“…The coatings obtained using these techniques displayed similar properties such as the retention of a high degree of monomer structure and functionality. Coatings deposited at lower plasma powers were not found to exhibit good stability in water, the use of higher powers were required to yield coatings exhibiting greater stability in aqueous solutions [16].These latter coatings were dominated by ester functionalities and were highly cross-linked.The enhanced stability of the ppAAc coatings however was found to be associated with a reduction in COOH functionality [14]. This is due to high fractionalisation of the acrylic acid monomer.…”

mentioning

confidence: 99%

Protein adhesion on water stable atmospheric plasma deposited acrylic acid coatings

Donegan

Dowling

2013

Surface and Coatings Technology

View full text Add to dashboard Cite

There is considerable interest in the application of plasma polymerised acrylic acid (ppAAc) coatings due to their ability to enhance the adhesion of cells and proteins. An issue with this coating however it its stability in water and previous studies carried out using low pressure plasmas have demonstrated that high plasma powers are required to achieve water stable coatings. In this paper the use of both helium and air atmospheric plasmas are compared for the deposition of ppAAc coatings. The deposition studies were carried out on silicon wafer substrates using the PlasmaStream TM and PlasmaTreat TM plasma jet deposition systems respectively. The coatings were characterized using contact angle, FTIR, SEM, XPS, ellipsometry and optical profilometry. While both the helium and air plasmas were successful in the deposition of ppAAc coatings, the nm thick films deposited using the PlasmaTreat system exhibited significantly higher levels of water stability, probably due to a higher level of coating cross linking. Ellipsometry measurements demonstrated only a 0.2 nm reduction in the thickness of an 18 nm thick ppAAc coating, when immersed in an aqueous buffer solution for one hour. Protein attachment studies were carried out using a flow cell system, which was monitored using a spectroscopic ellipsometer. This study was carried out with Bovine Serum Albumin (BSA), Immunoglobulin G (IgG) and Fibrinogen (Fg) proteins. In all three cases increased levels of protein adhesion was observed for the ppAAc coating, compared to that obtained on the uncoated silicon wafer substrates.Keywords: Atmospheric Plasma, Thin Film, Nano-coatings, Surface Engineering, Protein Adhesion IntroductionControlling protein adhesion is an important issue affecting many different fields including bio-processing, medical device implants, biosensors, and drug delivery devices [1,2]. When the surface of a material interacts with a biological environment one of the initial interactions is through protein adhesion [3]. Amongst the factors influencing protein adsorption, are surface chemical functionality and morphology [4]. Amongst the techniques that have been investigated to modify the surfaces of polymers prior to protein adhesion studies have been micro patterning and monomer polymerisation by free radical solution, emulsion, grafting as well as plasma processes [5][6][7][8][9]. It has been demonstrated by a number of authors that plasma techniques enable both the surface functionality and morphology to be tailored [10,11]. This makes it possible to create a surface which will inhibit or enhance the rate of protein adhesion onto a biomaterial surface, without changing the properties of the bulk material [12].One surface chemistry that has received considerable attention for cell and protein adhesion has been acrylic acid. Surfaces containing this coating have been reported in applications ranging from platelet adhesion promotion [13], RGD peptide immobilisation [14], attachment of osteoblast-like [15], fibroblast [16] and keratinocyte [17]...

show abstract

“…Some examples include permanent hydrophilic treatments of textiles or contact lenses and the formation of carboxylated surfaces which can serve as platforms for the immobilization of bioactive molecules for biointerfaces (in which case already low densities of functional groups are sufficient). [1][2][3][4][5] Although acrylic acid is often used as monomer for plasma polymerization, mixtures of hydrocarbons with carbon dioxide might show some advantages. [6,7] Experimental series using hydrocarbon monomers show a complex behaviour of the deposition rate as a function of the energy input, where often a drop in deposition rate is reported at higher plasma energies.…”

Section: Introductionmentioning

confidence: 99%