Stable Permanently Hydrophilic Protein-Resistant Thin-Film Coatings on Poly(dimethylsiloxane) Substrates by Electrostatic Self-Assembly and Chemical Cross-Linking

Makamba, Honest; Hsieh, Ya Yu; Sung, Wang Chou; Chen, Shuhui

doi:10.1021/ac0502706

Cited by 114 publications

(103 citation statements)

References 60 publications

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“…Building a polyelectrolyte multilayer (PEM) film coating on top of the PDMS surface increases surface wettability and imparts lasting hydrophilicity, thereby improving adhesion and proliferation of cells on PDMS surfaces. [31][32][33] This method holds promise because of the ease with which these films can coat PDMS surfaces, and the thickness of the films can easily be controlled. PEM is a strikingly simple method that allows formation of nanoscale structures by alternate adsorption of poly-anions and poly-cations on virtually any substrate.…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion on Polyelectrolyte Multilayer Coated Polydimethylsiloxane Surfaces with Varying Topographies

et al. 2007

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This article demonstrates that the micro-topography of the surface with respect to the pattern size and pitch influences cell adhesion and proliferation. Extensive research has shown the dependence of cell proliferation on substrate chemistry, but the influence of substrate topography on cell attachment has only recently been appreciated. To evaluate the effect of substrate physical properties (i.e., periodic microstructures) on cell attachment and morphology, we compared the response of several cell types (fibroblasts, HeLa, and primary hepatocytes) cultured on various polydimethylsiloxane (PDMS) patterns. PDMS has been used as an artificial construct to mimic biological structures. Although PDMS is widely used in biomedical applications, membrane technology, and microlithography, it is difficult to maintain cells on PDMS for long periods, and the polymer has proved to be a relatively inefficient substrate for cell adhesion. To improve adhesion, we built polyelectrolyte multilayers (PEMs) on PDMS surfaces to increase surface wettability, thereby improving attachment and spreading of the cells. Micrographs demonstrate the cellular response to physical parameters, such as pattern size and pitch, and suggest that surface topography, in part, regulates cell adhesion and proliferation. Therefore, varying the surface topography may provide a method to influence cell attachment and proliferation for tissueengineering applications.

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Section: Introductionmentioning

confidence: 99%

Cell Adhesion on Polyelectrolyte Multilayer Coated Polydimethylsiloxane Surfaces with Varying Topographies

et al. 2007

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“…More recently, Makamba et al [186] reported the grafting of PEG on a PDMS microchannel, in order to obtain a permanently hydrophilic surface suitable for electrophoretic separation of proteins. A hydrolyzed poly(styrenealt-maleic anhydride) base layer was first adsorbed on the oxidized PDMS.…”

Section: Polymer Brushes By Grafting To Strategymentioning

confidence: 99%

Surface treatment and characterization: Perspectives to electrophoresis and lab‐on‐chips

et al. 2006

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The control and modification of surface state is a major challenge in bioanalytical sciences, and in particular in electrokinetic separation methods, due to the importance of electroosmosis. This topic has gained recently a renewed interest, associated with the development of "lab-on-chips" systems that extend the range of materials in which separation channels are fabricated. The surface science community has developed through the years a large toolbox of characterization tools and surface modification protocols, which is not yet fully exploited in the bioanalytical world. In this paper, we try and present an overview of these tools, in order to stimulate new ideas for improved and more controlled surface treatment strategies for separations in capillaries and microchannels. We briefly describe some physical and chemical aspects of electroosmosis (global and spatially resolved), streaming current, and streaming potential. We also review the main strategies for surface coating, and compare the advantages of physisorption, well-organized thin self-assembled monolayers, or conversely thick polymer "brushes". Examples of existing applications to electrophoresis in microchannel are also given.

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“…Several other PDMS hydrophilic surface modification strategies have been explored, such as UV treatment [21,22], chemical vapor deposition [23], layer-by-layer (LBL) deposition [24,25], sol-gel coatings [26], silanization [27], dynamic modification with surfactants [28], and protein adsorption [29], but all result in the formation of what can be seen as a bilayer system unstable over time.…”

Section: Introductionmentioning

confidence: 99%

Chemical modification of PDMS surface without impacting the viscoelasticity: Model systems for a better understanding of elastomer/elastomer adhesion and friction

Dirany

Dies

Restagno

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. h i g h l i g h t s• Poly(dimethylsiloxane) surfaces were modified with poly(ethylene glycol).• Surface modification via hydrosilylation has no impact on viscoelastic properties.• Modified surfaces exhibit lower contact angle and higher surface energy. g r a p h i c a l a b s t r a c t a r t i c l e i n f oKeywords: Poly(dimethylsiloxane) elastomers Surface modification Hydrosilylation Contact angle Surface energy Viscoelastic properties a b s t r a c tThe influence of both viscoelastic and interfacial parameters on the surface properties of elastomers is difficult to study. Here, we describe a simple route to achieve surface modification of PDMS without impacting the viscoelastic properties of the bulk. PEG modified PDMS surfaces were synthesized by two step surface modification based on hydrosilylation. The covalent grafting of PEG on the surface has been evidenced by AFM and ATR-FTIR, and its effect on the hydrophilicity characterized by static and dynamic contact angle. The static water contact angle of the PEG-modified PDMS decreases from 110• (for unmodified PDMS) to 65• . Dynamic contact angles also show a significant decrease in both advancing and receding contact angles, along with a significant increase in the contact angle hysteresis, which can be related to an increase in the surface energy as estimated by JKR measurements. The viscoelastic properties of modified PDMS are found to be quantitatively comparable to those of the unmodified PDMS. This simple method is an efficient way to prepare model materials which can be used to get a better understanding of the exact contribution of the surface chemistry on surface properties of elastomers.

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Stable Permanently Hydrophilic Protein-Resistant Thin-Film Coatings on Poly(dimethylsiloxane) Substrates by Electrostatic Self-Assembly and Chemical Cross-Linking

Cited by 114 publications

References 60 publications

Cell Adhesion on Polyelectrolyte Multilayer Coated Polydimethylsiloxane Surfaces with Varying Topographies

Cell Adhesion on Polyelectrolyte Multilayer Coated Polydimethylsiloxane Surfaces with Varying Topographies

Surface treatment and characterization: Perspectives to electrophoresis and lab‐on‐chips

Chemical modification of PDMS surface without impacting the viscoelasticity: Model systems for a better understanding of elastomer/elastomer adhesion and friction

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