Stable nanocolloids of poorly soluble drugs with high drug content prepared using the combination of sonication and layer-by-layer technology

Agarwal, Anshul; Lvov, Yuri; Sawant, Rishikesh M.; Torchilin, Vladimir P.

doi:10.1016/j.jconrel.2008.03.017

Cited by 149 publications

(101 citation statements)

References 23 publications

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“…Delivery of hydrophilic and hydrophobic drugs is currently a great challenge in the field of nanobiotechnology, especially for water-insoluble organic compounds, which constitute a great part of the currently available drugs, whether anti-inflammatory or anti-cancer [1,2]. Indeed, these drugs tend to induce a systemic cytotoxicity due to high affinity towards lipid cell membranes and to spontaneously aggregate in the blood stream, ultimately reducing the bioavailability of the drug.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, the layer-by-layer assembly technique of polyelectrolytes onto a solid or biodegradable template to form polyelectrolyte multilayer films (PEMs) has recently emerged as a promising technique to engineer drug delivery systems [24][25][26][27]. Several strategies have already been proposed to trap and release cancer drugs, either by employing the film as a reservoir [28,29] or by coating preformed drug crystals [1,30] or porous silica templates containing the drug with PEM films [31]. Until now, few strategies have been reported for incorporation of poorly water-soluble molecules using PEM films.…”

Section: Introductionmentioning

confidence: 99%

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Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel

Boudou

Kharkar

Jing

et al. 2012

ASME 2012 Summer Bioengineering Conference, Parts a and B

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Efficient and effective delivery of poorly water-soluble drug molecules, which constitute a large part of commercially available drugs, is a major challenge in the field of drug delivery. Several drugs including paclitaxel (PTX) which are used for cancer treatment are hydrophobic, exhibit poor aqueous solubility and need to be delivered using an appropriate carrier. In the present work, we engineered Taxol-loaded polyelectrolyte films and microcapsules by pre-complexing PTX with chemically modified derivative of hyaluronic acid (alkylamino hydrazide) containing hydrophobic nanocavities, and subsequent assembly with either poly(L-lysine) (PLL) or quaternized chitosan (QCHI) as polycations. The PTX loading capacity of the films was found to be dependent on number of layers in the films as well as on the initial concentration of PTX precomplexed to hydrophobic HA, with a loading capacity up to 5000-fold the initial PTX concentration. The films were stable in physiological medium and were degraded in the presence of hyaluronidase. The PTX-loaded microcapsules were found to decrease the viability and proliferation of MDA MB 231 breast cancer cells, while unloaded microcapsules did not impact cell viability. All together, our results highlight the potential of hyaluronan-based assemblies containing hydrophobic nanodomains for hydrophobic drug delivery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel

Boudou

Kharkar

Jing

et al. 2012

ASME 2012 Summer Bioengineering Conference, Parts a and B

View full text Add to dashboard Cite

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“…The release profiles of FITC-dextran (20,40, and 70 kDa) from nanocapsules prepared with five alternating layers of alginate and chitosan at pH 7.4 and 4.5 are shown in Figure 5A,B, respectively. The nanocapsules displayed different release rates depending on the FITC-dextran molecular weight.…”

Section: Resultsmentioning

confidence: 99%

Loading and Protection of Hydrophilic Molecules into Liposome-Templated Polyelectrolyte Nanocapsules

Cuomo

Ceglie

Piludu³

et al. 2014

Langmuir

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Compartmentalized systems produced via the layer-by-layer (LbL) self-assembly method have been produced by alternatively depositing alginate and chitosan layers onto cores of liposomes. The combination of dynamic light scattering (DLS), ζ potential, and transmission electron microscopy (TEM) techniques provides detailed information on the stability, dimensions, charge, and wall thickness of these polyelectrolyte globules. TEM microphotographs demonstrate the presence of nanocapsules with an average diameter of below 300 nm and with a polyelectrolyte wall thickness of about 20 nm. The possibility of encapsulating and releasing molecules from this type of nanocapsule was demonstrated by loading FITC-dextrans of different molecular weights in the liposome system. The release of the loaded molecules from the nanocapsule was demonstrated after liposome core dissolution. Even at low molecular weight (20 kDa), the nanocapsules appear to be appropriate for prolonged molecule compartmentalization and protection. By means of the Ritger-Peppas model, non-Fickian transport behavior was detected for the diffusion of dextran through the polyelectrolyte wall. Values of the diffusion coefficient were calculated and yield useful information regarding chitosan/alginate hollow nanocapsules as drug-delivery systems. The influence of the pH on the release properties was also considered. The results indicate that vesicle-templated hollow polyelectrolyte nanocapsules show great potential as novel controllable drug-delivery devices for biomedical and biotechnological applications.

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“…For example, US FDI requirement of injections is set to the limitation that particles have to be less than 200 nm. In order to improve such practical difficulties, Lvov and coworkers developed a novel technique, sonicated LbL assembly, in which LbL polyelectrolyte shell is prepared under powerful ultrasonication [255,256]. This approach allows us to produce stable drug nanocolloids of high concentration and with good colloid stability.…”

Section: Hollow Capsulesmentioning

confidence: 99%

Enzyme-Encapsulated Layer-by-Layer Assemblies: Current Status and Challenges Toward Ultimate Nanodevices

Ariga

Hill

2010

Advances in Polymer Science

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Alternate layer-by-layer (LbL) adsorption has received much attention as an emerging methodology. Biocompatibility is the most prominent advantage of the LbL assembly process because the technique employs mild conditions for film construction. Most enzymes, especially water-soluble ones, have charged sites at their surfaces so that electrostatic LbL adsorption is suitable for construction of various protein organizations. In this review chapter, we summarize recent developments on enzyme-encapsulated LbL devices and their related functions where "encapsulated" does not always entail entrapment within spherical structures but generally includes immobilization of enzymes within the LbL structures. Recent examples, with various functions such as reactor sensors and medical applications, are described within a classification of structural types, i.e., thin films and spherical capsules. In addition to conventional applications, advanced systems including integration of LbL structures into advanced devices such as microchannels, field effect transistors, and flow injection amperometric sensors are introduced as well as recent developments in hybridization of LbL assemblies with functional nanomaterials such as carbon nanotube, dendrimers, nanoparticles, lipid assemblies, and mesoporous materials, all of which can enhance bio-related functions of LbL assemblies.

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Stable nanocolloids of poorly soluble drugs with high drug content prepared using the combination of sonication and layer-by-layer technology

Cited by 149 publications

References 23 publications

Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel

Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel

Loading and Protection of Hydrophilic Molecules into Liposome-Templated Polyelectrolyte Nanocapsules

Enzyme-Encapsulated Layer-by-Layer Assemblies: Current Status and Challenges Toward Ultimate Nanodevices

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