Stable male sterility induced by the expression of mutated melon ethylene receptor genes in Nicotiana tabacum

Ishimaru, Kentaro; Takada, Keita; Watanabe, Shin; Kamada, Hiroshi; Ezura, Hiroshi

doi:10.1016/j.plantsci.2006.04.006

Cited by 17 publications

(4 citation statements)

References 28 publications

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“…Previous studies on male sterility noted changes in ethylene and gibberellic acid (GA) content in GMS lines [47,48]. These genes were up-regulated by almost two-fold at the tetrad stage in the GMS mutant anther, but down-regulated at the meiosis and uninucleate microspore stages in the WT anther.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

et al. 2013

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BackgroundCotton (Gossypium hirsutum) anther development involves a diverse range of gene interactions between sporophytic and gametophytic tissues. However, only a small number of genes are known to be specifically involved in this developmental process and the molecular mechanism of the genetic male sterility (GMS) is still poorly understand. To fully explore the global gene expression during cotton anther development and identify genes related to male sterility, a digital gene expression (DGE) analysis was adopted.ResultsSix DGE libraries were constructed from the cotton anthers of the wild type (WT) and GMS mutant (in the WT background) in three stages of anther development, resulting in 21,503 to 37,352 genes detected in WT and GMS mutant anthers. Compared with the fertile isogenic WT, 9,595 (30% of the expressed genes), 10,407 (25%), and 3,139 (10%) genes were differentially expressed at the meiosis, tetrad, and uninucleate microspore stages of GMS mutant anthers, respectively. Using both DGE experiments and real-time quantitative RT-PCR, the expression of many key genes required for anther development were suppressed in the meiosis stage and the uninucleate microspore stage in anthers of the mutant, but these genes were activated in the tetrad stage of anthers in the mutant. These genes were associated predominantly with hormone synthesis, sucrose and starch metabolism, the pentose phosphate pathway, glycolysis, flavonoid metabolism, and histone protein synthesis. In addition, several genes that participate in DNA methylation, cell wall loosening, programmed cell death, and reactive oxygen species generation/scavenging were activated during the three anther developmental stages in the mutant.ConclusionsCompared to the same anther developmental stage of the WT, many key genes involved in various aspects of anther development show a reverse gene expression pattern in the GMS mutant, which indicates that diverse gene regulation pathways are involved in the GMS mutant anther development. These findings provide the first insights into the mechanism that leads to genetic male sterility in cotton and contributes to a better understanding of the regulatory network involved in anther development in cotton.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

et al. 2013

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“…Subsequently, expression of a ribonuclease inhibitor gene (barstar) from B. amyloliquifaciens in tapetal cells eliminated the ribonuclease activity and restored fertility in the male sterile lines (Mariani et al 1992). Using similar rationale and strategy, male sterile plants have been produced by expression of degradative enzymes (Worrall et al 1992;Kriete et al 1996;Cho et al 2001;Ruiz and Daniell 2005), by inhibition of particular enzymes by antisense strategy (van der Meer et al 1992;Groot et al 2004;Kapoor and Takatsuji 2006;Nawaz-ul-Rehman et al 2007;Ribarits et al 2007;Sandhu et al 2007), by expression of hormone encoding genes (Schmulling et al 1988), by expressing unedited form of mitochondrial genes in nucleus and targeting the product into mitochondria using a mitochondrial transit peptide Hernould et al 1998), by expressing the male sterility-associated mitochondrial DNA from well-characterized CMS systems (He et al 1996;Kim et al 2007;Yamamoto et al 2008), and also by expressing transcription factors and anther-speciWc receptors (Takada et al 2005;Ishimaru et al 2006;Mitsuda et al 2006;Li et al 2007). In many of these cases the restoration also has been achieved by selectively blocking the expression of the gene causing male sterility (Mariani et al 1992;Schmulling et al 1993;Kriete et al 1996;Zabaleta et al 1996;Bisht et al 2004;Busi et al 2006;Li et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression of sunflower cytoplasmic male sterility-associated open reading frame, orfH522 induces male sterility in transgenic tobacco plants

Nizampatnam

Doodhi

Narasimhan

et al. 2009

Planta

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Sterility in the universally exploited PET1-CMS system of sunflower is associated with the expression of orfH522, a novel mitochondrial gene. Definitive evidence that ORFH522 is directly responsible for male sterility is lacking. To test the hypothesis that ORFH522 is sufficient to induce male sterility, a set of chimeric constructs were developed. The cDNA of orfH522 was cloned in-frame with yeast coxIV pre-sequence, and was expressed under tapetum-specific promoter TA29 (construct designated as TCON). For developing control vectors, orfH522 was cloned without the transit peptide under TA29 promoter (TON) or orfH522 was cloned with or without transit peptide under the constitutive CaMV35S promoter (SCOP and SOP). Among several independent transformants obtained with each of the gene cassettes, one third of the transgenics (6/17) with TCON were completely male sterile while more than 10 independent transformants obtained with each of the control vectors were fertile. The male sterile plants were morphologically similar to fertile plants, but had anthers that remained below the stigmatic surface at anthesis. RT-PCR analysis of the sterile plants confirmed the anther-specific expression of orfH522 and bright-field microscopy demonstrated ablation of the tapetal cell layer. Premature DNA fragmentation and programmed cell death was observed at meiosis stage in the anthers of sterile plants. Stable transmission of induced male sterility trait was confirmed in test cross progeny. This constitutes the first report at demonstrating the induction of male sterility by introducing orfH522 gene that could be useful for genetic engineering of male sterility.

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“…In addition, numerous genes (e.g. mutated melon ethylene receptor gene) that are overepressed or underexpressed in the tapetum can cause male sterility (Ishimaru et al 2006). Overexpression of Zm401 probably functions in part to affect a cell death response that could also be triggered by many types of secondary factors.…”

Section: Zm401 Is Involved In a Crucial Regulation Network Controllinmentioning

confidence: 99%

Zm401p10, encoded by an anther-specific gene with short open reading frames, is essential for tapetum degeneration and anther development in maize

Wang

Zhao

et al. 2009

Functional Plant Biol.

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In flowering plants, the tapetum is proposed to play a vital role in the early stages of pollen development. Disruptions to tapetum development and degeneration typically result in male sterility. The present study characterised a maize (Zea mays L.) anther-specific gene, Zm401, which only contains short open reading frames (sORFs). The longest ORF of the Zm401 gene encodes a small protein designated Zm401p10 that accumulates in the nucleus. Overexpression of Zm401p10 in maize retarded tapetal degeneration and caused microspore abnormalities. A microarray analysis identified 278 downregulated and 150 upregulated genes in anthers overexpressing Zm401p10. These results indicate that the Zm401 gene is one of the major components of the molecular network regulating maize anther development and male fertility, and that Zm401p10 is expressed from the longest ORF of the gene.

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Stable male sterility induced by the expression of mutated melon ethylene receptor genes in Nicotiana tabacum

Cited by 17 publications

References 28 publications

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

Expression of sunflower cytoplasmic male sterility-associated open reading frame, orfH522 induces male sterility in transgenic tobacco plants

Zm401p10, encoded by an anther-specific gene with short open reading frames, is essential for tapetum degeneration and anther development in maize

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