Stable isotopes verify geographical origin of Tibetan chicken

Zhaxi, Cidan; Zhao, Shanshan; Zhang, Tangwei; Dong, Hao; Liu, Haijin; Zhao, Yan

doi:10.1016/j.foodchem.2021.129893

Cited by 20 publications

(11 citation statements)

References 33 publications

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“…The δ 2 H values of animal products are affected by drinking water and animal feed, which are related to latitude, altitude, and distance from the sea ( 28 ). In our study, the δ 2 H values of gelatin are −84.19 ± 8.76‰ in NMG, −62.94 ± 3.97‰ in SD, and −52.98 ± 3.90‰ in GX, indicating a significant difference.…”

Section: Resultsmentioning

confidence: 99%

“…The data were analyzed using SPSS 24.0. A post-hoc Duncan's test of a one-way analysis of variance (ANOVA) was performed to determine significant differences between the mean values of gelatin samples from different regions ( p < 0.05) ( 28 ). A paired sample t -test and Pearson's correlation analysis were, respectively, used to analyze the variability and correlation in δ 13 C, δ 15 N, and δ 2 H from bone to gelatin samples during processing to evaluate the effectiveness of these factors as geographical origin indicators ( p < 0.01) ( 20 , 29 ).…”

Section: Methodsmentioning

confidence: 99%

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Geographical traceability of gelatin in China using stable isotope ratio analysis

Jiang²,

Li³

et al. 2023

Front. Nutr.

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Geographical traceability is crucial to the quality and safety control of gelatin. However, currently, methods for gelatin traceability have not been established anywhere in the world. This study aimed to investigate the possibility of differentiating the geographical origins of gelatin from different regions in China using stable isotope technology. To achieve this objective, 47 bovine stick bone samples from three different regions (Inner Mongolia, Shandong, and Guangxi, respectively) in China were collected, and gelatin was extracted from these bones using the enzymatic method. The fingerprint characteristics of stable isotopes of δ13C, δ15N, and δ2H of gelatin from different regions in China were studied. Moreover, isotopic changes from the bone to gelatin during the processing were examined to evaluate the effectiveness of these factors as origin indicators. The results of the one-way analysis of variance (ANOVA) showed that the δ13C, δ15N, and δ2H of gelatin from different regions display significant differences, and using the linear discriminant analysis (LDA), the correct differentiation of origin reached 97.9%. Certain differences in stable isotope ratios were observed during the processing of bone to gelatin samples. Nonetheless, the fractionation effect caused by the processing of bone to gelatin samples was not sufficient to influence the identification of gelatin from different origins, which proves that δ13C, δ15N, and δ2H are effective origin indicators of gelatin. In conclusion, the stable isotope ratio analysis combined with the chemometric analysis can be used as a reliable tool for identifying gelatin traceability.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Geographical traceability of gelatin in China using stable isotope ratio analysis

Jiang²,

Li³

et al. 2023

Front. Nutr.

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“…TCs, the most important poultry breed in the Tibetan Plateau, possess several advantages, including excellent disease resistance, medicinal value, and high nutritional content. Moreover, this traditional food is known for its natural and healthy qualities, making it a sought-after product with limited availability and high market price (19). TCs were introduced to plain areas several years ago for large-scale intensive breeding, driven by market demand and economic benefits.…”

Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted February 20, 2024. ; https://doi.org/10.1101/2024.02. 19.581084 doi: bioRxiv preprint extreme cold, low oxygen levels, and strong UV radiation (3). In addition, certain species display distinct behaviors, such as seasonal migration to lower elevations to locate food and seek refuge from inclement weather, as well as burrowing to protect themselves from cold and wind (4).…”

Section: Introductionmentioning

confidence: 99%

Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages

Zeng,

Cao,

Yin

et al. 2024

Preprint

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Tibetan animals have several unique advantages owing to the harsh ecological conditions under which they live. Tibetan mammals have been studied extensively. However, understanding of the advantages and underlying mechanisms of the representative high-latitude bird, the Tibetan chicken (Gallus gallus, TC), remains limited. The gut microbiota of animals has been conclusively shown to be closely related to both host health and host environmental adaptation. This study aimed to explore the relationships between the cecal microbiome and the advantages of TCs based on comparisons among three populations: native TCs residing on the plateau, domestic TCs living in the plain, and one native plain species. Metatranscriptomic sequencing revealed a significant enrichment of active Bacteroidetes but a loss of active Firmicutes in native TCs. Additionally, the upregulated expression of genes in the cecal microbiome of native TCs showed enriched pathways related to energy metabolism, glycan metabolism, and the immune response. Furthermore, the expression of genes involved in the biosynthesis of short-chain fatty acids (SCFAs) and secondary bile acids (SBAs) was upregulated in the cecal microbiome of native TCs. Data from targeted metabolomics further confirmed elevated levels of certain SCFAs and SBAs in the cecum of native TCs. Based on the multi-omics association analysis, we proposed that the higher ratio of active Bacteroidetes/Firmicutes may be attributed to the efficient energy metabolism and stronger immunological activity of native TCs. Our findings provide a better understanding of the interactions between gut microbiota and highland adaptation, and novel insights into the mechanisms by which Tibetan chickens adapt to the plateau hypoxic environment.IMPORTANCEThe composition and function of the active cecal microbiome were significantly different between the plateau Tibetan chicken population and the plain chicken population. Higher expression genes related to energy metabolism and immune response were found in the cecal microbiome of the plateau Tibetan chicken population. The cecal microbiome in the plateau Tibetan chicken population exhibited higher biosynthesis of short-chain fatty and secondary bile acids, resulting in higher cecal content of these metabolites. The active Bacteroidetes/Firmicutes ratio in the cecal microbiome may contribute to the high-altitude adaptive advantage of the plateau Tibetan chicken population.

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“…The stable isotope ratio mass spectrometry (IRMS) technique can precisely determine the stable isotope ratios of carbon, hydrogen, oxygen, nitrogen, and sulfur. It has been widely used in geology [1,2], food science [3,4], environmental protection [5,6], forensic science [7,8], and other fields. It has also been employed in the comparison and discrimination of AN [9].…”

Section: Introductionmentioning

confidence: 99%

Determination of stable nitrogen isotopic ratios of nitrate ions in ammonium nitrate

Huang

Mei

et al. 2021

Journal of Forensic Sciences

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Ammonium nitrate (AN) is one of the most commonly used explosives in criminal cases. The comparison and source‐tracing of AN is important for investigation of attribution and fingerprinting of an explosive used at different events. The stable isotope signature of AN is an important index for comparison and tracing. However, the characteristics of the stable nitrogen isotopic ratios of AN (δ15NNH4NO3) alone are not sufficient to achieve a fine comparison between different AN samples. To increase the comparison index and further improve the discriminability between stable nitrogen isotopic ratios of different ANs, a method of isolation and analysis of nitrate ions in AN was established using stable‐isotope‐ratio mass spectrometry (IRMS). The method was based on the principle that strong alkali react with AN to produce ammonia and nitrate. After the isolation, stable nitrogen isotopes of nitrate ions (δ15NNO3) were obtained using IRMS, and then the stable nitrogen isotopes of ammonium ions from AN (δ15NNH4) was calculated according to the principle of mass balance. The results show that the method is effective for the isolation of nitrate ions without notable isotope fractionation. The developed method was applied to analyze and discriminate AN samples from eight different cities in China. Three samples out of the initial eight AN samples with similar δ15NNH4NO3 values were further distinguished by their δ15NNH4 and δ15NNO3 values. The isolation and stable‐nitrogen isotopic analysis method developed for nitrate ions in AN is simple and effective, thereby increasing the discriminability of the stable isotope ratios in AN.

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Stable isotopes verify geographical origin of Tibetan chicken

Cited by 20 publications

References 33 publications

Geographical traceability of gelatin in China using stable isotope ratio analysis

Geographical traceability of gelatin in China using stable isotope ratio analysis

Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages

Determination of stable nitrogen isotopic ratios of nitrate ions in ammonium nitrate

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