“…These early efforts were continued in 20th century, with varying degrees of success, mostly due to difficulties in building reliable chronologies of ice accumulation . Thus, cave ice-based studies addressed the geochemistry and stable isotope geochemistry of cave ice (Kern et al, 2009;Kern et al, 2011a;Kern et al, 2011b;May et al, 2011), the dynamics of past winter (Perșoiu et al, 2017) and summer (Bădăluţă et al, 2020) air temperatures, past environmental and vegetation changes (Feurdean et al, 2011;Sancho et al, 2018;Leunda et al, 2019) and the dynamics of cave ice accumulations (Stoffel et al, 2009;Perșoiu and Pazdur, 2011;Spötl et al, 2014;. Over the past few years, several studies investigated microorganism in cave ice deposits, with specifically designed drilling strategies (Sattler et al, 2013;Itcus et al, 2018) allowing the recovery of millennia-old microbial (Mondini et al, 2019;Paun et al, 2019) and fungal (Brad et al, 2018) communities.…”