Stable isotope studies on the oxidation of ammonia to hydroxylamine by <i>nitrosomonas europaea</i>

Dua, Rishabh; Bhandari, Basant; Nicholas, D. J. D.

doi:10.1016/0014-5793(79)80541-4

Cited by 78 publications

(56 citation statements)

References 7 publications

(7 reference statements)

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“…NH: oxidizer activity was determined by measuring dark 14C-HCO, uptake with and without nitrapyrin (2-chloro-6-trichloromethyl pyridine), a specific inhibitor of NH: oxidizing bacteria (Billen 1976, Somville 1978, Belser & Schmidt 1981. Nitrapynn interferes with the initial biochemical step in NH: oxidation, catalyzed by NH: oxidase, where NH: is converted to hydroxylamine (Dua et al 1979, Hooper & Terry 1979. Ten m1 cell suspensions from melted cores or 130 m1 seawater were preincubated in 20 m1 scintillation vials or 130 m1 borosilicate glass stoppered bottles, respectively, with nitrapyrin (10 mg I-' final concentration) dissolved in 90 % ethanol (0.08 '10 final ethanol concentration) or with 0.08 ?h ethanol controls for 1 h in the dark.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

Priscu¹,

Downes²,

Priscu³

et al. 1990

Mar. Ecol. Prog. Ser.

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M3STRACT Nitrapynn, an lnh~bitor of NH; oxldlz~ng bactena was used to estlmate the a c t~v~t y of NH: oxldlzing bacteria in the bottom 1 to 15 cm of annual sea Ice and in the water column at vanous locations In McMurdo Sound and along the Ross Ice Shelf (RIS) N~t r a p y r~n s~gn~flcantly ~n h~b i t e d dark ' 4 C -H C 0 3 uptake in virtually all sea-ice samples, indlcahng the presence of NH: oxld~zlng bacteria Inorganic carbon futahon by sea ice NH: o x~d~z e r s was only a small fract~on of that futed by sea-lce photoautotrophs, both on an hourly and annual b a s~s Despite their relative lack of ~mportance to lnorganlc carbon fixation NH: oxidizing bactena may have an important role in the N d y n a m~c s w~t h l n the b~o g e n i c layer of annual sea Ice both In terms of N H~ utlllzatlon and eventual NO; product~on Inorgamc carbon futatlon in the water column beneath sea ice was generally not i n h~b~t e d s~gnif~cantly by nitrapynn NH: oxldizer actlvlty was also not detectable in deep water flow~ng beneath (southward) or from under (northward) the RIS N 2 0 (a by-product of NH: oxidation) levels in p e l a g~c samples were always near 100 % of saturation w t h respect to the alr above the sea surface corroborating the low levels of NH:ox~dizer activity found In the water column

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Section: Methodsmentioning

confidence: 99%

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

Priscu¹,

Downes²,

Priscu³

et al. 1990

Mar. Ecol. Prog. Ser.

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“…Based on the results in [2] and [3] an atom of oxygen from dioxygen is added to ammonia to form hydroxylamine. We note that oxygenation of ammonia may involve production of water (i.e., typical of a monooxygenase) or the incorporation of both atoms of dioxygen into an N-substrate (i.e., typical of a dioxygenase).…”

Section: Or (Ii) 15~180180-------t 15~160180-_ 15~160160-_mentioning

confidence: 99%

“…Authors in [l] achieved incorporation by cells of 7% of one nitrite-oxygen from "OZ as analyzed by mass spectrometry. In [2] and [3] it was demonstrated that cellular oxidation of ammonia to hydroxylamine, a probable intermediate in NO; production, involves incorporation of oxygen from dioxygen. Thus, assuming that hydroxylamine is an intermediate and that the oxygen of hydroxylamine is not lost by hydrolysis, at least one of the two oxygens of NOi is from dioxygen.…”

Section: Introductionmentioning

confidence: 99%

O₂ and H₂O are each the source of one O in NO⁻₂ produced from NH₃ by Nitrosomonas: ¹⁵N‐NMR evidence

Andersson¹,

Hooper²

1983

FEBS Letters

333

161

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The exchange of "0 between Hz*'0 and exogenously added '5N'602 which occurs during oxidation of ammonia by Nitrosomonas is shown to occur one oxygen at a time. Conditions in which the exchange is diminished (notably the presence of 14N02 and CCCP) allowed demonstration that water and dioxygen are each the source of one oxygen in nitrite produced from "NH3. The nitrite produced in the presence of "02 consisted of 67 and 0% 15N180160-and 1sN180180-, respectively. Analysis was made using the '80-isotope shift in "N-NMR.Nitrosomonas

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“…Corresponding to this widely spread occurrence their metabolism is flexible; they gain energy using three different pathways, one oxic and two anoxic. The mechanism of aerobic ammonia oxidation was the first pathway discovered (Rees & Nason, 1966;Hooper, 1969a;Hyman & Wood, 1985;Arciero & Hooper, 1993;Hyman & Arp, 1993;Bergmann et al, 1994;Sayavedra-Soto et al, 1994;Dua et al, 1979;Anderson & Hooper, 1983). Later, Abeliovich & Vonshak (1992) and Bock et al (1995) described a heterotrophic and hydrogen-dependent denitrification as an alternative pathway.…”

Section: Introductionmentioning

confidence: 99%

Ammonium and hydroxylamine uptake and accumulation in Nitrosomonas

et al. 2004

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Starved cells of Nitrosomonas europaea and further ammonia oxidizers were able to rapidly accumulate ammonium and hydroxylamine to an internal concentration of about 1 and 0·8 M, respectively. In kinetic studies, the uptake/accumulation rates for ammonium [3·1 mmol (g protein)−1 min−1] and hydroxylamine [4·39 mmol (g protein)−1 min−1] were determined. The uptake and accumulation process of ammonium and hydroxylamine was not coupled to ammonia or hydroxylamine oxidation and nitrite was not produced. In the presence of uncouplers the ammonium accumulation was completely inhibited, indicating an active, membrane-potential-driven transport mechanism. When the external ammonium or hydroxylamine pool was depleted, the internal ammonium and hydroxylamine was consumed within 12 h or 20 min, respectively. The binding of ammonium/ammonia was correlated with an energized membrane system, and hydroxylamine may bind to the hydroxylamine oxidoredutase.

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Stable isotope studies on the oxidation of ammonia to hydroxylamine by nitrosomonas europaea

Cited by 78 publications

References 7 publications

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

O₂ and H₂O are each the source of one O in NO⁻₂ produced from NH₃ by Nitrosomonas: ¹⁵N‐NMR evidence

Ammonium and hydroxylamine uptake and accumulation in Nitrosomonas

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Stable isotope studies on the oxidation of ammonia to hydroxylamine by nitrosomonas europaea

Cited by 78 publications

References 7 publications

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

Dynamics of ammonium oxidizer activity and nitrous oxide (N20) within and beneath Antarctic sea ice

O2 and H2O are each the source of one O in NO−2 produced from NH3 by Nitrosomonas: 15N‐NMR evidence

Ammonium and hydroxylamine uptake and accumulation in Nitrosomonas

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O₂ and H₂O are each the source of one O in NO⁻₂ produced from NH₃ by Nitrosomonas: ¹⁵N‐NMR evidence